A5001152129- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Ovarian function and disorders
- Reproductive Health and Technologies
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Veterinary Equine Medical Research
- Seed Germination and Physiology
- Renal and related cancers
- Veterinary Medicine and Surgery
- Primate Behavior and Ecology
- Selenium in Biological Systems
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Reproductive System and Pregnancy
- Mitochondrial Function and Pathology
- Risk and Safety Analysis
- Plant Toxicity and Pharmacological Properties
- Xenotransplantation and immune response
- Disaster Management and Resilience
- Microbial infections and disease research
- Bee Products Chemical Analysis
- Hormonal and reproductive studies
University of California, Davis
2015-2024
Foothill College
2022
Division of Undergraduate Education
2021
University of Calgary
2012
Universidade Federal de Minas Gerais
2012
University of California Davis Medical Center
2008
Institute of Cell Biology and Neurobiology
2005-2007
University of Pennsylvania
1998-2000
University of Nebraska–Lincoln
1996
University of Illinois Urbana-Champaign
1992
ABSTRACT: The objective of this study was to examine the effect reactive oxygen species (ROS) and cryopreservation on DNA fragmentation equine spermatozoa. In experiment 1, spermatozoa were incubated (1 hour, 38°C) according following treatments: 1) sperm alone; 2) + xanthine (X, 0.3 mM)‐xanthine oxidase (XO, 0.025 U/mL); 3) × (0.6 mM)‐XO (0.05 4) (0.1 U/mL). 2, with U/mL) either catalase (200 U/mL), superoxide dismutase (SOD, 200 or reduced glutathione (GSH, 10 mM). Following incubation,...
Testicular maturation and sperm production throughout the life of male form basis fertility. It is difficult to elucidate intricate processes controlling testicular spermatogenesis in primates vivo due long time span required for sexual also lack accessible vitro or models primate spermatogenesis. Ectopic xenografting neonatal testis tissue into mice provides an model study manipulate propagation differentiation germ cells from immature donor animals. However, it was not clear whether this...
Significance Maternal inheritance of mitochondria and mitochondrial genes is a major developmental paradigm in mammals. Propagation paternal, sperm-contributed genes, resulting heteroplasmy, seldom observed mammals, due to postfertilization targeting degradation sperm mitochondria, referred as “sperm mitophagy.” Our others’ recent results suggest that mitophagy mediated by the ubiquitin–proteasome system, protein-turnover pathway degrades proteins autophagic pathway. Here we demonstrate...
Objective: While tomato product supplementation, containing antioxidant carotenoids, including lycopene, decreases oxidative stress, the role of purified lycopene as an remains unclear. Thus, we tested effects different doses supplementation on biomarkers stress in healthy volunteers.Methods: This was a double-blind, randomized, placebo-controlled trial, examining 8-week plasma levels, lipid peroxidation {LDL oxidizability, malondialdehyde & hydroxynonenals (MDA HNE), urinary...
Stallion sperm rely primarily on oxidative phosphorylation for production of ATP used in motility and metabolism. The objective the study was to identify which substrates included Biggers, Whitten, Whittingham (BWW) media are key optimal mitochondrial function through measurements parameters, oxygen consumption, cellular reactive species (ROS) production. It expected that substrates, pyruvate lactate, would support better than glycolytic substrate, glucose, due direct utilization within...
Phosphorylation of tyrosine residues on sperm proteins is one important intracellular mechanism regulating function that may be a meaningful indicator capacitation. There substantial evidence cryopreservation promotes the capacitation and this cryocapacitation frequently cited as factor associated with reduced longevity cryopreserved in female reproductive tract. This study was designed to determine whether stallion express different levels phosphorylation after vitro thawed display similar...
Cryopreservation introduces extreme temperature and osmolality changes that impart lethal sublethal effects on spermatozoa survival. Additionally, evidence indicates the osmotic stress induced by cryopreservation causes oxidative to as well. Our objective was determine effect of reactive oxygen species (ROS) rhesus macaque (Macaca mulatta) sperm function whether elicits production ROS. In first experiment, xanthine-xanthine oxidase (X-XO) system used generate ROS superoxide anion (O2−·)...
Laboratories around the world have produced tens of thousands mutant and transgenic zebrafish lines. As with mice, maintaining all these valuable genotypes is expensive, risky, beyond capacity even largest stock centers. Because reducing oxidative stress has become an important aspect variability in mouse sperm cryopreservation, we examined whether antioxidants might improve cryopreservation sperm. Four experiments were conducted this study. First, used xanthine-xanthine oxidase (X-XO)...
Mammalian sperm that have completed capacitation are capable of undergoing the acrosome reaction in response to a number biological and chemical stimuli. In present report, we investigated ability progesterone stimulate reactions stallion capacitated vitro. Motile were selected by two-layer Percoll gradient centrifugation incubated TALP medium modified 1:1 (v/v) addition TEST-yolk for 5 hours at 39 degrees C, under 5% CO2 humidified air. Sperm vitro TALP-TEST had higher percentage following...
The plasma membrane of sperm can undergo lipid phase separation during freezing, resulting in irreversible damage to the cell. objective our study was examine behavior equine spermatozoa absence and presence lipid-based cryoprotectants. Biophysical properties membranes were investigated with Fourier-transform infrared spectroscopy. Compared fresh untreated sperm, postthaw showed extensive rearrangement. In contrast, that cryopreserved egg phosphatidylcholine (egg PC)- or soy (soy PC)-based...
In juvenile monkeys, precocious puberty can be induced by administration of gonadotropins resulting in testicular somatic cell maturation and germ differentiation. It is, however, unknown whether also younger monkeys. Here we used testis tissue xenografting to investigate infant monkey will undergo and/or spermatogenesis response endogenous adult mouse or exogenous gonadotropins. Testicular pieces from 3- 6-month-old rhesus monkeys were grafted immunodeficient, castrated mice. Recipient mice...
Optical trapping is a non-invasive biophysical tool which has been widely applied to study physiological and biomechanical properties of cells. Using laser ‘tweezers’ in combination with custom-designed computer tracking algorithms, the swimming speeds relative forces individual sperm can be measured real time. This physical engineering tools used examine evolutionary effect competition primates. The results demonstrate correlation between mating type motility: from polygamous...
Human embryos resulting from abnormal early cleavage can result in aneuploidy and failure to develop normally the blastocyst stage. The nature of paternal influence on embryo development has not been directly demonstrated although many studies have suggested effects spermatozoal chromatin packaging, DNA damage, centriolar mitotic spindle integrity plasma membrane integrity. goal this study was determine whether developmental events were affected by oxidative damage fertilizing sperm....
During transit through the female reproductive tract, mammalian spermatozoa are exposed to increasing concentrations of progesterone (P4) released by cumulus oophorus. P4 triggers massive calcium influx into human sperm activation sperm-specific channel CatSper. These properties thought be unique since CatSper is not sensitive in rodent sperm. Here, performing patch clamp recording from rhesus macaque for first time, we report that they express P4-sensitive identically and react inducing...
Coral reefs are experiencing unprecedented degradation due to human activities, and protecting specific reef habitats may not stop this decline, because the most serious threats global (i.e., climate change), local. However, ex situ preservation practices can provide safeguards for coral conservation. Specifically, modern advances in cryobiology genome banking could secure existing species genetic diversity until genotypes be introduced into rehabilitated habitats. We assessed feasibility of...
ABSTRACT: Single‐cell gel electrophoresis (SCGE), or comet assay, has the ability to detect damage at single cell level and not been reported for equine sperm. The nuclear could aid in advancement of protocols optimal semen preservation. goals these experiments were adapt this assay use with sperm utilize determining integrity DNA following treatments storage various decreased temperatures (‐20°C 5°C). Results from which frozen (‐20°C) absence cryoprotectants revealed that significantly more...