A5024340967- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Insect and Pesticide Research
- Viral Infections and Outbreaks Research
- Respiratory viral infections research
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Influenza Virus Research Studies
- Plant Virus Research Studies
- Viral Infections and Vectors
- Disaster Response and Management
- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
- Long-Term Effects of COVID-19
- Dermatological and COVID-19 studies
- Plant and animal studies
- RNA Interference and Gene Delivery
- COVID-19 epidemiological studies
- vaccines and immunoinformatics approaches
- COVID-19 diagnosis using AI
- interferon and immune responses
- PARP inhibition in cancer therapy
University of Liverpool
2018-2025
Health & Life (Taiwan)
2025
Northwest A&F University
2024
University of Oxford
2023
University of Bristol
2023
The Pirbright Institute
2023
Food and Drug Administration
2023
Jiangsu Normal University
2017-2021
Genomics (United Kingdom)
2021
Xi’an Jiaotong-Liverpool University
2016-2018
In life-threatening coronavirus disease (COVID-19), corticosteroids reduce mortality, suggesting that immune responses have a causal role in death. Whether this deleterious inflammation is primarily direct reaction to the presence of severe acute respiratory syndrome 2 (SARS-CoV-2) or an independent immunopathologic process unknown.
A novel coronavirus, SARS-CoV-2, has been identified as the causative agent of current COVID-19 pandemic. Animal models, and in particular non-human primates, are essential to understand pathogenesis emerging diseases assess safety efficacy vaccines therapeutics. Here, we show that SARS-CoV-2 replicates upper lower respiratory tract causes pulmonary lesions both rhesus cynomolgus macaques. Immune responses against also similar species equivalent those reported milder infections convalescent...
SARS-CoV-2 has a broad mammalian species tropism infecting humans, cats, dogs, and farmed mink. Since the start of 2019 pandemic, several reverse zoonotic outbreaks have occurred in mink, one which reinfected humans caused cluster infections Denmark. Here we investigate molecular basis mink ferret adaptation demonstrate spike mutations Y453F, F486L, N501T all specifically adapt to use mustelid ACE2. Furthermore, risk assess these conclude mink-adapted viruses are unlikely pose an increased...
Abstract Background The mutational landscape of SARS-CoV-2 varies at the dominant viral genome sequence and minor genomic variant population. During COVID-19 pandemic, an early substitution in was D614G change spike protein, associated with increase transmissibility. Genomes are accompanied by a P323L polymerase (NSP12). However, is not thought to be under strong selective pressure. Results Investigation P323L/D614G substitutions population shows rapid emergence during containment phase...
COVID-19 is a spectrum of clinical symptoms in humans caused by infection with SARS-CoV-2. The coalescence SARS-CoV-2 seasonal respiratory viruses, particularly influenza global health concern. To understand this, transgenic mice expressing the human ACE2 receptor (K18-hACE2) were infected A virus (IAV) followed and host response effect on biology was compared to K18-hACE2 IAV or alone. sequentially showed reduced RNA synthesis, yet exhibited more rapid weight loss, severe lung damage...
In infected individuals, viruses are present as a population consisting of dominant and minor variant genomes. Most databases contain information on the genome sequence. Since emergence SARS-CoV-2 in late 2019, variants have been selected that more transmissible capable partial immune escape. Currently, models for projecting evolution based using sequences to forecast whether known mutation will be prevalent future. However, novel (and other viruses) driven by evolutionary pressure acting...
Abstract COVID-19 is a complex disease phenotype where the underlying microbiome could influence morbidity and mortality. Amplicon metagenomic MinION based sequencing was used to rapidly (within 8 hours) identify SARS-CoV-2 assess in nasopharyngeal swabs obtained from patients with by ISARIC 4C consortium.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of disease 2019 (COVID-19). Sequencing viral genome as outbreak progresses important, particularly in identification emerging isolates with different pathogenic potential and to identify whether nucleotide changes will impair clinical diagnostic tools such real-time PCR assays. Although single polymorphisms point mutations occur during replication coronaviruses, one biggest drivers genetic change...
Molnupiravir is an antiviral, currently approved by the UK Medicines and Healthcare products Regulatory Agency (MHRA) for treating at-risk COVID-19 patients, that induces lethal error catastrophe in SARS-CoV-2. How this drug-induced mechanism of action might impact emergence resistance mutations unclear. To investigate this, we used samples from AGILE Candidate Specific Trial (CST)-2 (clinical trial number NCT04746183). The primary outcomes CST-2 were to measure drug safety antiviral...
The number of managed honey bee colonies has considerably decreased in many developed countries recent years and ectoparasitic mites are considered as major threats to health. However, their general biology remains poorly understood. We sequenced the genome Tropilaelaps mercedesae, prevalent mite infesting bees Asia, predicted 15 190 protein-coding genes that were well supported by transcriptomes proteomic data. Although amino acid substitutions have been accelerated within conserved core...
Abstract Background Trombidid mites have a unique life cycle in which only the larval stage is ectoparasitic. In superfamily Trombiculoidea (“chiggers”), larvae feed preferentially on vertebrates, including humans. Species genus Leptotrombidium are vectors of potentially fatal bacterial infection, scrub typhus, that affects 1 million people annually. Moreover, chiggers can cause pruritic dermatitis (trombiculiasis) humans and domesticated animals. Trombidioidea (velvet mites), other...
Abstract Background SARS-CoV-2 remains rapidly evolving, and many biologically important genomic substitutions/indels have characterised novel lineages, which emerged during successive global waves of the pandemic. Worldwide sequencing has been able to monitor these waves, track transmission clusters, examine viral evolution in real time help inform healthcare policy. One school thought is that an apparent greater than average divergence emerging lineage from contemporary variants may...
Recent honey bee colony losses, particularly during the winter, have been shown to be associated with presence of both ectoparasitic mites and Deformed Wing Virus (DWV). Whilst role Varroa destructor as a viral vector is well established, Tropilaelaps mercedesae in transmission has not fully investigated. In this study, we tested effects that V. T. infestation on fluctuation DWV copy number alteration virus variants bees by characterizing individual pupae their infesting mites. We observed...
ABSTRACT Outbreaks of influenza A viruses are generally seasonal and cause annual epidemics worldwide. Due to their frequent reassortment evolution, surveillance is paramount importance guide vaccine strategies. The aim this study was explore the molecular epidemiology virus nasopharyngeal microbiota composition in infected patients Saudi Arabia. total 103 samples from 2015 12 2022 were collected positive for A. Sequencing as well metatranscriptomic analysis conducted using Oxford Nanopore...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has a complex strategy for the transcription of viral subgenomic mRNAs (sgmRNAs), which are targets nucleic acid diagnostics. Each these sgmRNAs unique 5′ sequence, leader–transcriptional regulatory sequence gene junction (leader–TRS junction), that can be identified using sequencing. High-resolution sequencing been used to investigate biology SARS-CoV-2 and host response in cell culture animal models from clinical...
Abstract Background Viral load is a major contributor to outcome in patients with Ebola virus disease (EVD), high values leading fatal outcome. Evidence from the 2013–2016 (EBOV) outbreak indicated that different genotypes of can have phenotypes patients. Additionally, due error-prone nature viral RNA synthesis an individual patient, EBOV genome exists around dominant sequence. The minor variants within patient may contribute overall phenotype terms protein function. To investigate effects...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic infection that emerged in the 2012. Symptoms range from mild to severe and include both gastrointestinal illnesses. The virus mainly present camel populations with occasional spill over into humans. severity of humans influenced by numerous factors, similar acute 2 (SARS-CoV-2), underlying health complications can play major role. Currently, MERS-CoV SARS-CoV-2 are coincident thus rapid way sequencing derive genotype...
SARS-CoV-2 is the causative agent of COVID-19. The virus has spread across planet, causing a global pandemic.
Abstract COVID-19 is a spectrum of clinical symptoms in humans caused by infection with SARS-CoV-2, recently emerged coronavirus that rapidly pandemic. Coalescence this virus seasonal respiratory viruses, particularly influenza global health concern. To investigate this, transgenic mice expressing the human ACE2 receptor driven epithelial cell cytokeratin-18 gene promoter (K18-hACE2) were first infected IAV followed SARS-CoV-2. The host response and effect on biology was compared to...
Background The COVID-19 pandemic has created pressure on healthcare systems worldwide. Tools that can stratify individuals according to prognosis could allow for more efficient allocation of resources and thus improved patient outcomes. It is currently unclear if blood gene expression signatures derived from patients at the point admission hospital provide useful prognostic information. Methods Gene whole obtained a cohort 78 hospitalised with during first wave was measured by high...
The worldwide COVID-19 pandemic has claimed millions of lives and had a profound effect on global life. Understanding the body's immune response to SARS-CoV-2 infection is crucial in improving patient management prognosis. In this study we compared influenza infected cohorts identify distinct blood transcript abundances cellular composition better understand natural associated with COVID-19, another viral being influenza, prognostic signature outcome. Clinical characteristics peripheral were...
Abstract Background Ebola virus disease (EVD) is an often-fatal infection where the effectiveness of medical countermeasures uncertain. During West African outbreak (2013–2016), several patients were treated with different types anti-viral therapies including monoclonal antibody-based cocktails that had potential to neutralise (EBOV). However, at time, efficacy these was Given scale outbreak, clinical phenotypes came forefront ability same cause recrudescence in patient—perhaps through...
The transient receptor potential cation channel, subfamily A, member 1 (TRPA1) is conserved between many arthropods, and in some has been shown to function as a chemosensor for noxious compounds. Activation of arthropod TRPA1 channels by temperature fluctuations tested only few insect species, all them were be activated heat. recent identification chemosensitive from two honey bee ectoparasitic mite species (VdTRPA1 TmTRPA1) have provided an opportunity study the temperature-dependent...
Filoviruses cause high-consequence infections with limited approved medical countermeasures (MCMs). MCM development is dependent upon well-characterized animal models for the assessment of antiviral agents and vaccines. Following large-scale Ebola virus (EBOV) disease outbreaks in Africa, some survivors are left long-term sequelae persistent immune-privileged sites many years. We report characterization ferret as a model infection, reproducing lethality observed humans. The onset clinical...
Ipomoea is the largest genus in family Convolvulaceae. The species this have been widely used many fields, such as agriculture, nutrition, and medicine. With development of next-generation sequencing, more than 50 chloroplast genomes sequenced. However, repeats divergence regions not well investigated. In present study, we sequenced assembled eight from sweet potato's close wild relatives. By combining these with 32 published genomes, conducted a detailed comparative analysis broad range...