A5100739552- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Liver Disease Diagnosis and Treatment
- HIV-related health complications and treatments
- Hepatitis B Virus Studies
- Trace Elements in Health
- Heavy Metal Exposure and Toxicity
- Liquid Crystal Research Advancements
- Tuberculosis Research and Epidemiology
- Metabolomics and Mass Spectrometry Studies
- S100 Proteins and Annexins
- HIV/AIDS Research and Interventions
- Liver physiology and pathology
- Antibiotics Pharmacokinetics and Efficacy
- Immune Response and Inflammation
- Pneumocystis jirovecii pneumonia detection and treatment
- interferon and immune responses
- Mosquito-borne diseases and control
- Endoplasmic Reticulum Stress and Disease
- Alcohol Consumption and Health Effects
- Bone health and osteoporosis research
- Advanced Materials and Mechanics
- Nuclear Structure and Function
- Viral Infections and Immunology Research
- Hydrogen's biological and therapeutic effects
Guangdong Ocean University
2025
Shanghai Innovative Research Center of Traditional Chinese Medicine
2025
Shanghai Public Health Clinical Center
2012-2024
National Institute for Viral Disease Control and Prevention
2024
Center for Disease Control
2024
Shandong University
2024
Fudan University
2007-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
XinHua Hospital
2023
Shanghai Jiao Tong University
2023
Salt stress significantly suppresses rice growth. Uniconazole (S3307) is recognized for its potential to enhance plant tolerance. Nevertheless, the mechanisms through which S3307 induces salt tolerance in by modulating carbon metabolism pathway are not fully understood. In this study, at one-leaf-one-heart stage, foliage of HD961 and 9311 was treated with 10 mg·L- 1 S3307, followed a 0.6% (102.56 mmol·L- 1) NaCl treatment 24 h later. The results demonstrated that markedly suppressed growth...
The human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. To better understand the influence of on host cells, we performed differential proteome research peripheral blood mononuclear cells (PBMCs) from HIV-positive patients and healthy controls. 26 protein spots with more than 1.5-fold difference were detected in two dimensional electrophoresis (2DE) gels. 12 unique up-regulated one down-regulated proteins identified compared donors. mRNA...
Abstract It is important to study the mechanism of liver fibrogenesis, and find new non‐invasive biomarkers. In this study, we used subcellular proteomic technology plasma membrane (PM) proteins related immune fibrosis search for A rat model was induced by pig serum injection. The fibrogenesis from stage (S) S0‐1, S2, S3‐4, S4 detected Masson staining HE in after 2, 4, 6, 8 weeks treatment. PM enriched analyzed using technology. differentially expressed were verified Western blotting,...
Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. To identify new biomarkers for early diagnosis of OS novel therapeutic candidates, we carried out a plasma membrane proteomic study based on two-dimensional electrophoresis (2DE). The cell line MG-63 human osteoblastic hFOB1.19 were adopted as comparison model. We extracted by aqueous two-phase partition extraction. proteins separated through 2DE. analyzed differentially expressed Imagemaster...
Abstract The individual HLA-related susceptibility to emerging viral diseases such as COVID-19 underscores the importance of understanding how HLA polymorphism influences peptide presentation and T cell recognition. Similar HLA-A*0101, which is one earliest identified alleles among human population, HLA-A*2601 possesses a similar characteristic for binding acts prevalent allomorph in HLA-I. In this study, we found that, compared with individuals exhibit distinctive features responses...
Proteins are attractive building blocks for fabricating diverse and precise nanomaterials. However, the facile fabrication of multidimensional artificial assemblies is highly challenging. Here, inspired by large-scale production technique inorganic nanomaterials, we demonstrate application liquid phase exfoliation (LPE) on native protein ConA design synthetic ligands. These ligands provide distinct in-plane out-of-plane supramolecular interactions, allowing generation architectures based...
The incidence rate of tuberculosis (TB) in patients with human immunodeficiency virus (HIV) infection is 26 times higher than that other patients. Patients both infections require long-term combination therapy, which increases therapy complexity and might lead to serious adverse reactions drug-drug interactions. To optimize for HIV TB coinfection, we developed an ultra-high-performance liquid chromatography/tandem mass spectrometry (UHPLC-MS/MS) method simultaneously quantify four...
Abstract Novel tools are necessary to explore proteins related human immunodeficiency virus (HIV) infection. In this work, proteomic and glycoproteomic technology were employed examine plasma samples from HIV‐positive patients. Through comparative proteome analysis of normal samples, 19 differentially expressed protein spots 12 non‐redundant identified by ESI‐ion trap MS. Among these, the 130‐kDa isoform α‐1‐antitrypsin was found be decreased in patients while another variant with a...
Abstract Background Patients with immunodeficiency virus-1 (HIV-1) infection are challenging to be cured completely due the existence of HIV-1 latency reservoirs. However, knowledge mechanisms and biomarkers associated is limited. Therefore, identifying proteins related could provide new insights into underlying latency, ultimately contribute eradication HIV Methods An Isobaric Tags for Relative Absolute Quantification (iTRAQ)-labeled subcellular proteomic study was performed on an latently...
Abstract Lopinavir (LPV) is a protease inhibitor (PI) for the treatment of human immunodeficiency virus (HIV) infections. Current studies on LPV are mainly focused Caucasians, and none have investigated population pharmacokinetics (PPK) in Chinese population. The present study aimed to develop PPK model oral adults who HIV‐infected. A total 460 concentrations from 174 patients received LPV/ritonavir (LPV/r) 400/100 mg orally every 12 hours (q12h) were analysed using non‐linear mixed‐effects...
Elucidation of the mechanisms liver fibrogenesis is important to treat fibrosis. In this study, we established rat models fibrosis with stages from 0–1, 2, and 3–4 4 at 4, 6, 8 weeks, respectively, by injection pig serum. Liver was detected Masson's trichrome staining. Rat non-parenchymal cells (NPCs) were enriched 4-fold Percoll density gradient centrifugation. Protein extracts NPCs prepared separated two-dimensional electrophoresis, then stained Coomassie Blue G-250. At identified 18...
Due to the persistence of latent HIV-infected cellular reservoirs, HIV virus can not be eradicated completely.To identify proteins related latency, we performed a subcellular proteomic study in cell lines.An established HIV-1 model (J-Lat Tat-GFP Clone A7 cells, cells) and its parental line (Jurkat were used. The plasma membrane (PM) fraction from cultured cells was enriched through aqueous two-phase partition. PM extracted then separated using two-dimensional electrophoresis (2DE)....
ObjectivesHuman immunodeficiency virus (HIV) and hepatitis B (HBV) share similar routes of transmission, rapid progression hepatic diseases has been observed in coinfected individuals. Our main objective was to investigate the molecular mechanism HIV/HBV coinfections.MethodsWe selected HIV infected patients with without Highly Active Antiretroviral Therapy (HAART). Low abundance proteins enriched using a multiple affinity removal system (MARS) were labeled isobaric tags for relative absolute...
Xiaofang Jia1, Li Liu1, Xiaoqian Dage Wu1, Lin Yin1, Xiaohui Liu2, Jiaoli Zhang1, Pengyuan Yang2, Hongzhou Lu1*, and Lijun Zhang1* Shanghai Public Health Clinical Center, Fudan University, 201508, China Institutes of Biomedical Sciences, 200032, *Correspondence address. Tel: þ86-21-37990333-5368; Fax: þ86-21-57248073; E-mail: zhanglijun1221@163.com (L.Z.). þ86-21-37990333; luhongzhou@fudan.edu.cn (H.L.)
Alcohol-induced injury has become one of the major causes for liver cirrhosis. However, molecular mechanisms ethanol-induced are not fully understood. To this end, we performed a dynamic plasma membrane proteomic research on rat model. A model from hepatitis to cirrhosis was developed. Plasma tissue with fibrosis stage 2 and 4 (S2 S4) purified by sucrose density gradient centrifugation. Its purification verified western blotting. Proteins were separated two-dimensional electrophoresis (2DE)...
Background Efavirenz (EFV), a non-nucleoside reverse transcriptase inhibitor (NNRTI), has been widely used in the therapy of human immunodeficiency virus (HIV) infection. Some its toxic effects on hepatic cells have reported to display features mitochondrial dysfunction through bioenergetic stress and autophagy, etc. However, alteration protein levels, especially during treatment EFV not fully investigated. Methods We built cell model EFV-induced liver toxicity treating Huh-7 with different...
Background Lamivudine is a first-line medication used for human immunodeficiency virus (HIV) treatment. To date, the population pharmacokinetics of lamivudine in Chinese HIV-infected adults have not been assessed. This study aimed to develop pharmacokinetic model oral and determine optimal dosage regimens.Research design methods A total 1113 samples, from 828 patients treated with 300 mg every 24 hours, were pooled two open-label, prospective clinical trials. analysis was performed using...
Enterovirus A71 (EV71) infection can cause hand, foot, and mouth disease (HFMD) severe neurological complications in children. However, the biological processes regulated by EV71 remain poorly understood. Herein, proteomics metabonomics studies were conducted to uncover mechanism of rhabdomyosarcoma (RD) cells identify potential drug targets. Differential expressed proteins from enriched membrane analyzed isobaric tags for relative absolute quantitation (iTRAQ)-based technology. Twenty-six...
Objective: Efavirenz (EFV) is commonly used in combination antiretroviral therapy (cART). However, our previous study, many persons living with human immunodeficiency virus (HIV) exhibited ocular complications despite undergoing effective cART. Here, we aimed to determine the intraocular EFV concentrations vitreous and analyze factors affecting viral load patients HIV-associated retinopathies. Design: Observational, retrospective study. Methods: Fourteen receiving an who underwent pars plana...