A5084828848- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Dental Implant Techniques and Outcomes
- 3D Printing in Biomedical Research
- Nanoplatforms for cancer theranostics
- Calcium Carbonate Crystallization and Inhibition
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Nanoparticle-Based Drug Delivery
- Photodynamic Therapy Research Studies
- Electrospun Nanofibers in Biomedical Applications
- biodegradable polymer synthesis and properties
- Cancer, Hypoxia, and Metabolism
- Orthopaedic implants and arthroplasty
- Polymer Surface Interaction Studies
- TGF-β signaling in diseases
- Fibroblast Growth Factor Research
- Tissue Engineering and Regenerative Medicine
- Bone health and osteoporosis research
- Dental materials and restorations
- Cancer Cells and Metastasis
- Composite Material Mechanics
- Bone and Dental Protein Studies
- Connective tissue disorders research
- Powder Metallurgy Techniques and Materials
UConn Health
2014-2024
University of Connecticut
2009-2021
University of Leeds
2016-2020
Case Western Reserve University
1995-2020
Cornell University
2016-2020
Orthopaedic Research Society
2014
Hartford Financial Services (United States)
2014
Hartford Hospital
2014
Harvard University
1998-2009
Boston Children's Hospital
1996-2009
The inorganic phase of bone is comprised primarily very small mineralites. size and shape these mineralites play fundamental roles in maintaining ionic homeostasis the biomechanical function bone. Using atomic force microscopy, we have obtained direct three-dimensional visual evidence native protein-free isolated from mature bovine Approximately 98% are less than 2 nm thick displaying a plate-like habit. Distributions both thickness width show single peaks. distribution lengths may be...
Controlled differentiation of human embryonic stem cells (hESCs) and induced pluripotent (iPSCs) into that resemble adult mesenchymal (MSCs) is an attractive approach to obtain a readily available source progenitor for tissue engineering. The present study reports new method rapidly derive MSC-like from hESCs hiPSCs, in one step, based on culturing the thin, fibrillar, type I collagen coatings mimic structure physiological collagen. Human H9 ESCs HDFa-YK26 iPSCs were singly dissociated...
Abstract The Raman spectrum of polycrystalline vaterite is presented and compared to spectra calcite aragonite, the other two common CaCO3 polymorphs; spectroscopy easily distinguishes between these three polymorphs. An important feature splitting both ν1 ν4 peaks. peak implies distinct site symmetries for CO3 −2 groups. A definitive crystal structure determination not yet available, but none proposed structures show such a feature.
Bioactive implants intended for rapid, robust, and durable bone tissue regeneration are presented. The based on nanofibrous 3D-scaffolds of bioresorbable poly-ϵ-caprolactone mimicking the fibrillar architecture matrix. Layer-by-layer nanoimmobilization growth factor BMP-2 in association with chitosan (CHI) or poly-L-lysine over nanofibers is described. osteogenetic potential scaffolds coated layers CHI demonstrated vitro, vivo mouse calvaria, through enhanced osteopontin gene expression...
A new system for the local delivery of chemotherapy to malignant solid tumors has been developed based on calcium phosphate (CaP) nanoparticles. The adsorption anti-neoplastic drug cis-diamminedichloroplatinum (cisplatin) was characterized three types apatitic CaP (poorly and well crystallized hydroxyapatite, carbonated apatite). Adsorption isotherms obtained in chloride-free solutions at pH = 7.4 (24 37 degrees C) indicate that cisplatin increases with temperature decreasing crystallinity....
The in vivo osteogenesis potential of mesenchymal-like cells derived from human embryonic stem (hESC-MCs) was evaluated by implantation on collagen/hydroxyapatite scaffolds into calvarial defects immunodeficient mice. This study is novel because no osteogenic or chondrogenic differentiation protocols were applied to the prior implantation. After 6 weeks, X-ray, microCT, and histological analysis showed that hESC-MCs had consistently formed a highly vascularized new bone bridged defect...
Combined regimens of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) were investigated to stimulate osteogenic differentiation. In young mouse calvaria-derived cells, FGF-2 (0.16ng/mL) in combination with BMP-2 (50ng/mL) did not enhance mineralization, but old cells it resulted more mineralization than alone. long cultures, enhanced relative alone, lower dose (0.016ng/mL) was necessary. neonatal calvarial sequential delivery low-dose (5ng/mL) stimulatory...
A drug delivery coating for synthetic bone grafts has been developed to provide sequential of multiple osteoinductive factors better mimic aspects the natural regenerative process. The is composed a biomimetic calcium phosphate (bCaP) layer that applied graft and then covered with poly-l-Lysine/poly-l-Glutamic acid polyelectrolyte multilayer (PEM) film. Bone morphogenetic protein-2 (BMP-2) was before process directly on then, bCaP-PEM deposited followed by adsorption fibroblast growth...
Abstract The purpose of this study was three‐fold: (a) to develop a new small animal model evaluate dental implant systems that recapitulates aspects the challenging intraoral environment, (b) screen several scaffolds for in vivo bone forming efficacy when used deliver non‐glycosylated morphogenetic protein‐2 (BMP‐2) together with miniaturized titanium (Ti) implant, and (c) identify correlations between vitro BMP‐2 release rates results. tested were: (1) collagen‐hydroxyapatite composite...
We have developed a novel nitroimidazole indocyanine dye conjugate for tumor-targeted hypoxia fluorescence tomography. The probe has been evaluated in vitro using tumor cell lines and vivo with targeting mice. studies were performed to assess labeling differences between normoxia conditions. When treated the probe, emission ratio of 2.5-fold was found cells incubated under compared condition. Hypoxia specificity also confirmed by comparing alone. In mice showed that signals measured at site...
Tumor hypoxia is a major indicator of treatment resistance to chemotherapeutic drugs, and fluorescence optical tomography has tremendous potential provide clinically useful, functional information by identifying tumor hypoxia. The synthesis 2-nitroimidazole-indocyanine green conjugate using piperazine linker (piperazine-2-nitroimidazole-ICG) capable robust fluorescent imaging described. In vivo mouse studies were completed demonstrate an improved capability the new dye relative earlier...