A5019018711- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Angiogenesis and VEGF in Cancer
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Adipose Tissue and Metabolism
- Caveolin-1 and cellular processes
- Cancer, Hypoxia, and Metabolism
- Lipoproteins and Cardiovascular Health
- Lipid metabolism and disorders
- TGF-β signaling in diseases
- Cardiac Fibrosis and Remodeling
- Nitric Oxide and Endothelin Effects
- Orthopedic Surgery and Rehabilitation
- Adipokines, Inflammation, and Metabolic Diseases
- Electrospun Nanofibers in Biomedical Applications
- Pharmaceutical Economics and Policy
- Signaling Pathways in Disease
- Renal and related cancers
- Cell Adhesion Molecules Research
- Fatty Acid Research and Health
- Congenital heart defects research
- Nuclear Receptors and Signaling
- Eicosanoids and Hypertension Pharmacology
Kentucky Science Center
2025
University of Louisville
2015-2024
University of Louisville Hospital
2024
Cardiovascular Innovation Institute
2011-2020
University of Georgia
2006-2009
The Wallace H. Coulter Department of Biomedical Engineering
2002-2004
Georgia Institute of Technology
2004
Emory University
2004
The University of Texas Medical Branch at Galveston
1999
Recently, we have shown that shear stress stimulates NO<sup>⋅</sup> production by the protein kinase B/Akt (Akt)-dependent mechanisms in bovine aortic endothelial cells (BAEC) (Go, Y. M., Boo, C., Park, H., Maland, M. Patel, R., Pritchard, K. A., Jr., Fujio, Y., Walsh, K., Darley-Usmar, V., and Jo, H. (2001) <i>J. Appl. Physiol.</i> 91, 1574–1581). Akt has been believed to regulate shear-dependent of directly phosphorylating nitric-oxide synthase (eNOS) at Ser<sup>1179</sup> residue...
Atherosclerosis is an inflammatory disease occurring preferentially in arterial regions exposed to disturbed flow conditions including oscillatory shear stress (OS). OS exposure induces endothelial expression of bone morphogenic protein 4 (BMP4), which turn may activate intercellular adhesion molecule-1 (ICAM-1) and monocyte adhesion. also known induce by producing reactive oxygen species (ROS) from reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, raising the possibility...
Atherosclerosis is now viewed as an inflammatory disease occurring preferentially in arterial regions exposed to disturbed flow conditions, including oscillatory shear stress (OS), branched arteries. In contrast, the laminar (LS) are relatively lesion-free. The mechanisms underlying opposite effects of OS and LS on atherogenic processes not clearly understood. Here, through DNA microarrays, protein expression, functional studies, we identify bone morphogenic 4 (BMP4) a mechanosensitive...
Caveolae are plasmalemmal domains enriched with cholesterol, caveolins, and signaling molecules. Endothelial cells in vivo continuously exposed to shear conditions, their caveolae density location may be different from that of static cultured cells. Here, we show chronic exposure regulates formation localization caveolin-1 bovine aortic endothelial (BAEC). Chronic (1 or 3 days) BAEC laminar increased the total number by 45–48% above control. This increase was due a rise luminal without...
Exposure to microgravity causes bone loss in humans, and the underlying mechanism is thought be at least partially due a decrease formation by osteoblasts. In present study, we examined hypothesis that changes osteoblast gene expression profiles, resulting loss. For this developed an vitro system simulates using Random Positioning Machine (RPM) study effects of on 2T3 preosteoblast cells grown gas-permeable culture disks. simulated RPM for up 9 days significantly inhibited alkaline...
Human embryonic stem cells (hESC) have the potential to produce all of in body. They are able self-renew indefinitely, potentially making them a source for large-scale production therapeutic cell lines. Here, we developed monolayer differentiation culture that induces hESC (WA09 and BG01) form epithelial sheets with mesodermal gene expression patterns (BMP4, RUNX1, GATA4). These E-cadherin+ CD90low then undergo apparent epithelial–mesenchymal transition derivation mesenchymal progenitor...
Abstract Human embryonic stem cells (hESCs) have recently demonstrated the potential for differentiation into germ-like in vitro. This provides a novel model understanding human germ cell development and infertility. Mouse fibroblast (MEF) feeders basic growth factor (bFGF) are two sources of signaling that essential primary culture cells, yet their role has not been examined derivation from hESCs. Here protein gene expression both MEF bFGF can significantly enrich Under enriched conditions,...
Familial hypercholesterolemia (FH) is a hereditary disease primarily due to mutations in the low-density lipoprotein receptor (LDLR) that lead elevated cholesterol and premature development of cardiovascular disease. Homozygous FH patients (HoFH) with two dysfunctional LDLR alleles are not as successfully treated standard hypercholesterol therapies, more aggressive therapeutic approaches control levels must be considered. Liver transplant can resolve HoFH, hepatocyte transplantation has...
Subverting the host immune response to inhibit inflammation is a key virulence strategy of Yersinia pestis . The inflammatory cascade tightly controlled via sequential action lipid and protein mediators inflammation. Because delayed essential for Y cause lethal infection, defining mechanisms manipulate necessary understand this pathogen’s virulence. While previous studies have established that actively inhibits expression proteins mediate inflammation, there currently gap in our...
One of the major challenges in cell implantation therapies is to promote integration microcirculation between implanted cells and host. We used adipose-derived stromal vascular fraction (SVF) vascularize a human liver (HepG2) implant. hypothesized that SVF would form functional via assembly inosculation with host vasculature. Initially, we assessed extent character neovasculatures formed by freshly isolated cultured found have higher vascularization potential. Generation 3D implant...
We report here that in rat and human neuroprogenitor cells as well embryonic cortical neurons Zika virus (ZIKV) infection leads to ribosomal stress is characterized by structural disruption of the nucleolus. The anti-nucleolar effects were most pronounced postmitotic neurons. Moreover, latter system, nucleolar presence ZIKV capsid protein (ZIKV-C) was associated with apoptosis. Deletion 22 C-terminal residues ZIKV-C prevented localization, Consistent a casual relationship between...
The in vivo osteogenesis potential of mesenchymal-like cells derived from human embryonic stem (hESC-MCs) was evaluated by implantation on collagen/hydroxyapatite scaffolds into calvarial defects immunodeficient mice. This study is novel because no osteogenic or chondrogenic differentiation protocols were applied to the prior implantation. After 6 weeks, X-ray, microCT, and histological analysis showed that hESC-MCs had consistently formed a highly vascularized new bone bridged defect...
Organ health and function depend on communication between cell types to coordinate tissue growth repair. Recent studies have indicated that fibroblasts are critical this process; however, their role in regulating inflammatory responses injury remained ambiguous. Here, we demonstrate transforming factor β-activated kinase 1 (TAK1) is a gatekeeper of the cardiac fibroblast phenotype. We find TAK1 propagates IL-1β TNF-α signaling coordinates synthesis secretion chemokines as well pro-resolving...
The objective of this study was to develop a novel in vitro model for smooth muscle cell (SMC) differentiation from human embryonic stem cell-derived mesenchymal cells (hES-MCs). We found that hES-MCs were differentiated SMCs by transforming growth factor-β (TGF-β) dose- and time-dependent manner as demonstrated the expression SMC-specific genes α-actin, calponin, myosin heavy chain. Under normal conditions, however, capacity very limited. hES-MC-derived had an elongated spindle-shaped...
Abstract Acquiring sufficient amounts of high-quality cells remains an impediment to cell-based therapies. Induced pluripotent stem (iPSC) may be unparalleled source, but autologous iPSC likely retain deficiencies requiring correction. We present a strategy for restoring physiological function in genetically deficient utilizing the low-density lipoprotein receptor (LDLR) deficiency Familial Hypercholesterolemia (FH) as our model. FH fibroblasts were reprogrammed into using synthetic modified...
Abstract The tumor microenvironment (TME) is composed of a heterogeneous biological ecosystem cellular and non-cellular elements including transformed cells, endothelial immune activated fibroblasts or myofibroblasts, stem progenitor as well the cytokines matrix that they produce. constituents TME stroma are multiple varied, however cancer associated (CAF) their contribution to important in progression. CAF hypothesized arise from cell types, mesenchymal cells. Currently, isolation patients...
Human embryonic stem cell–derived neural progenitors (NP) present an important tool for understanding human development and disease. Optimal utilization of NP cells, however, requires enhanced ability to monitor these cells in vitro vivo. Here we report production the first genetically modified self-renewing that express fluorescent proteins under constitutive as well lineage-specific promoters, enabling tracking monitoring cell fate. Nucleofection, transfection, lentiviral transduction were...
Microvascular mural or perivascular cells are required for the stabilization and maturation of remodeling vasculature. However, much less is known about their biology function compared to large vessel smooth muscle cells. We have developed lines multipotent mesenchymal from human embryonic stem (hES-MC); we hypothesize that these can as Here show derived do not form teratomas in SCID mice independently similar patterns gene expression by microarray analysis. When exposed platelet-derived...
Abstract Human mesenchymal stem cells (hMSC) have proven beneficial in the repair and preservation of infarcted myocardium. Unfortunately, MSCs represent a small portion bone marrow require ex vivo expansion. To further advance clinical usefulness cellular cardiomyoplasty, derivation “MSC‐like” that can be made available “off‐the‐shelf” are desirable. Recently, human embryonic cell‐derived (hESC‐MC) were described. We investigated efficacy hESC‐MC for cardiac after myocardial infarction (MI)...