A5016501769- Angiogenesis and VEGF in Cancer
- Coronary Interventions and Diagnostics
- Atherosclerosis and Cardiovascular Diseases
- Aortic aneurysm repair treatments
- Nitric Oxide and Endothelin Effects
- Tissue Engineering and Regenerative Medicine
- Bone and Dental Protein Studies
- Renin-Angiotensin System Studies
- Cardiovascular Health and Disease Prevention
- Mesenchymal stem cell research
- Aortic Disease and Treatment Approaches
- Cell Adhesion Molecules Research
- Electrospun Nanofibers in Biomedical Applications
- Bone Metabolism and Diseases
- Hemoglobinopathies and Related Disorders
- Eicosanoids and Hypertension Pharmacology
- Protease and Inhibitor Mechanisms
- Cardiovascular Function and Risk Factors
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cellular Mechanics and Interactions
- Cardiac Fibrosis and Remodeling
- Infective Endocarditis Diagnosis and Management
- Adipokines, Inflammation, and Metabolic Diseases
- Redox biology and oxidative stress
- Advanced Glycation End Products research
Emory University
2016-2025
The Wallace H. Coulter Department of Biomedical Engineering
2014-2025
Georgia Institute of Technology
2015-2025
Atlanta VA Medical Center
2013-2025
Veterans Health Administration
2013-2023
Emory and Henry College
2012-2021
American Heart Association
2020
Victor Chang Cardiac Research Institute
2020
Atlanta Medical Center
2005-2018
Istituto Ortopedico Rizzoli
2018
NAD(P)H oxidases are important sources of superoxide in the vasculature, activity which is associated with risk factors for human atherosclerosis. This study was designed to investigate localization production and expression Nox family oxidase proteins (gp91phox, Nox1, Nox4) nonatherosclerotic atherosclerotic coronary arteries.In artery segments from explanted hearts, we examined intracellular dihydroethidium. In arteries, present homogenously throughout intima, media, adventitia. there an...
Abstract —Recent evidence suggests that oxidative mechanisms may be involved in vascular smooth muscle cell (VSMC) hypertrophy. We previously showed angiotensin II (Ang II) increases superoxide production by activating an NADH/NADPH oxidase, which contributes to In this study, we determined whether Ang stimulation of oxidase results H 2 O studying the effects on intracellular generation, dismutase and catalase activity, (100 nmol/L) significantly increased levels at 4 hours. Neither activity...
Recent studies suggest that superoxide production by the NADPH/NADH oxidase may be involved in smooth muscle cell growth and pathogenesis of hypertension. We previously showed angiotensin II (Ang II) activates a p22phox-based cultured rat vascular cells animals made hypertensive infusion Ang II. To investigate mechanism responsible for this increased activity, we examined p22phox mRNA expression rats implanting an osmotic minipump delivered (0.7 mg/kg per day). Blood pressure began to...
Reactive oxygen species (ROS) have been implicated in the development of cardiovascular pathologies. NAD(P)H oxidases (Noxes) are major sources reactive vessel wall, but importance individual Nox homologues specific layers vascular wall is unclear. Nox1 upregulation has pathologies such as hypertension and restenosis.To investigate pathological role smooth muscle, transgenic mice overexpressing muscle cells (TgSMCnox1) were created, impact on medial hypertrophic response during angiotensin...
Accurate and tunable: The title compounds can detect reactive oxygen species (ROS) in cell culture, tissue explants, for the first time vivo. hydrocyanines are synthesized by reduction of cyanine dyes with NaBH4. They accurately nanomolar levels ROS, have excellent stability against autoxidation, tunable emission wavelengths range 560–830 nm. Detailed facts importance to specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset....
Background— The pathogenesis of hypertension remains poorly understood, and treatment is often unsuccessful. Recent evidence suggests that the adaptive immune response plays an important role in this disease. Various hypertensive stimuli cause T-cell activation infiltration into target organs such as vessel kidney, which promotes vascular dysfunction blood pressure elevation. Classically, requires receptor ligation costimulation. latter involves interaction between B7 ligands (CD80 CD86) on...
Therapeutic vascularization remains a significant challenge in regenerative medicine applications. Whether the goal is to induce vascular growth ischemic tissue or scale up tissue-engineered constructs, ability of patent, stable vasculature critical obstacle. We engineered polyethylene glycol–based bioartificial hydrogel matrices presenting protease-degradable sites, cell-adhesion motifs, and factors vivo. Compared injection soluble VEGF, these delivered sustained vivo levels VEGF over 2...
Background —Angiotensin II may contribute to the development and progression of atherosclerotic lesions because its growth proinflammatory effects. We sought determine whether angiotensin II–induced hypertension would augment accelerate in apoE-deficient mice. Methods Results (0.7 mg · kg −1 d SC) was administered mice via osmotic minipumps. The animals were placed on either standard chow or an atherogenic diet. After 8 weeks, mean lesion area descending thoracic abdominal aortas a diet...
Atherosclerosis is now viewed as an inflammatory disease occurring preferentially in arterial regions exposed to disturbed flow conditions, including oscillatory shear stress (OS), branched arteries. In contrast, the laminar (LS) are relatively lesion-free. The mechanisms underlying opposite effects of OS and LS on atherogenic processes not clearly understood. Here, through DNA microarrays, protein expression, functional studies, we identify bone morphogenic 4 (BMP4) a mechanosensitive...
Objective— The hemodynamic environment is a determinant of susceptibility to atherosclerosis in the vasculature. Although mouse models are commonly used studies, little known about local variations wall shear stress (WSS) and whether levels WSS comparable those humans. objective this study was determine values aorta relate these expression gene products associated with atherosclerosis. Methods Results— Using micro-CT ultrasound methodologies we developed computational fluid dynamics model...
Rationale: Repair of damaged endothelium is important in recovery from acute lung injury. In animal models, bone marrow–derived endothelial progenitor cells differentiate into mature and assist repairing vasculature.Objectives: The quantity patients with injury unknown. We hypothesize that increased numbers circulating will be associated an improved outcome the respiratory distress syndrome.Methods: Peripheral blood mononuclear buffy coat early (n = 45), intubated control subjects 10),...
Monocyte chemoattractant protein-1 (MCP-1), a potent monocyte synthesized by vascular cells and monocytes, has been proposed to be an important mediator of inflammatory responses in the arterial vasculature. It was recently demonstrated that hypertension is associated with response wall. To determine effect on MCP-1 expression, we induced Sprague-Dawley rats infusing angiotensin II (0.75 mg x kg[-1] d[-1] SC) for 7 days. Using Northern blot analysis, detected 3.6-fold increase mRNA aortas...
Transgenic mouse models are increasingly being used to investigate the functions of specific growth factors or matrix proteins design therapeutic strategies for controlling blood vessel growth. However, available methodologies evaluating angiogenesis and arteriogenesis in these limited by animal size, user subjectivity, power visualize three-dimensional networks, capability employ a vigorous quantitative analysis. In this study, we employed contrast-enhanced microcomputed tomography imaging...
Abstract OPN is an ECM protein with diverse localization and functionality. The role of during fracture healing was examined using wildtype OPN−/− mice. Results showed that plays important in regulation angiogenesis, callus formation, mechanical strength early stages facilitates late stage bone remodeling organization. Introduction: Osteopontin (OPN) extracellular matrix (ECM) functionality has been reported to play a regulatory both angiogenesis osteoclastic remodeling, two vital processes...
Background Stem cells for cardiac repair have shown promise in preclinical trials, but lower than expected retention, viability, and efficacy. Encapsulation is one potential strategy to increase viable cell retention while facilitating paracrine effects. Methods Results Human mesenchymal stem ( hMSC ) were encapsulated alginate attached the heart with a hydrogel patch rat myocardial infarction MI model. Cells tracked using bioluminescence BLI function measured by transthoracic...
Technologies to increase tissue vascularity are critically important the fields of engineering and cardiovascular medicine. Currently, limited technologies exist encourage angiogenesis arteriogenesis in a controlled manner. In present study, we describe an injectable release system consisting VEGF encapsulated poly(lactic- co-glycolic acid) (PLGA) nanoparticles (NPs). The majority was released gradually over 2–4 days from NPs as determined by ELISA kinetics experiment. An vitro aortic ring...
We recently reported that lowering of macrophage free intracellular iron increases expression cholesterol efflux transporters ABCA1 and ABCG1 by reducing generation reactive oxygen species. In this study, we explored whether levels via pharmacological suppression hepcidin can increase macrophage-specific reduce atherosclerosis.To suppress hepcidin, the exporter ferroportin, iron, used a small molecule inhibitor bone morphogenetic protein (BMP) signaling, LDN 193189 (LDN). (10 mg/kg IP...
A positron emission tomography (PET) tracer composed of (18)F-labeled maltohexaose (MH(18)F) can image bacteria in vivo with a sensitivity and specificity that are orders magnitude higher than those fluorodeoxyglucose ((18)FDG). MH(18)F detect early-stage infections as few 10(5) E. coli colony-forming units (CFUs), identify drug resistance vivo. has the potential to improve diagnosis bacterial given its unique combination high for bacteria.
Rationale: Low circulating progenitor cell numbers and activity may reflect impaired intrinsic regenerative/reparative potential, but it remains uncertain whether this translates into a worse prognosis. Objectives: To investigate low of cells associate with greater risk mortality in population at high cardiovascular risk. Methods Results: Patients undergoing coronary angiography were recruited 2 cohorts (1, n=502 2, n=403) over separate time periods. Progenitor enumerated by flow cytometry...