A5002334268- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Adenosine and Purinergic Signaling
- Prion Diseases and Protein Misfolding
- Neuroinflammation and Neurodegeneration Mechanisms
- Liver physiology and pathology
- RNA regulation and disease
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Cell Adhesion Molecules Research
- Nuclear Receptors and Signaling
- Nerve injury and regeneration
- Neurological diseases and metabolism
- Immune Response and Inflammation
- Autophagy in Disease and Therapy
- Neurological Complications and Syndromes
- Viral Infectious Diseases and Gene Expression in Insects
- Chemical Synthesis and Analysis
- Neuroscience of respiration and sleep
- Pancreatic function and diabetes
- Stress Responses and Cortisol
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- Neuropeptides and Animal Physiology
- Immune cells in cancer
Institute of Agrobiological Sciences
2016-2025
National Agriculture and Food Research Organization
2016-2025
Fuchu Hospital
2010-2011
Tokyo Metropolitan Institute of Medical Science
2008-2010
Institute of Applied Biochemistry
1992-2006
University of Tsukuba
1992-2006
National Institute of Animal Health
2006
University of California, San Diego
1999-2004
Zero to Three
2003
α-Synuclein is a major component of aggregates forming amyloid-like fibrils in diseases with Lewy bodies and other neurodegenerative disorders, yet the mechanism by which α-synuclein intracellularly aggregated during neurodegeneration poorly understood. Recent studies suggest that oxidative stress reactions might contribute to abnormal aggregation this molecule. In context, main objective present study was determine potential role heme protein cytochrome <i>c</i> aggregation. When...
The expression of α-synuclein, a synaptic molecule implicated in the pathogenesis neurodegenerative disorders such as Parkinson's disease and Lewy body is increased upon injury to nervous system, indicating that it might play role regeneration plasticity; however, mechanisms are unclear. Because c-Jun N-terminal kinase (JNK), member mitogen-activated protein family, plays an important stress response, main objective present study was better understand involvement this pathway signaling...
The involvement of microglia in neuroinflammatory responses has been extensively demonstrated. Recent animal studies have shown that exposure to either acute or chronic stress induces robust microglial activation the brain. In present study, we investigated underlying mechanism brain by stress.We first looked at spatial distribution noradrenaline (NA)-synthesizing enzyme, DBH (dopamine β-hydroxylase), comparison with NA receptors-β1, β2, and β3 adrenergic receptors (β1-AR, β2-AR,...
Recently, autophagy has been associated with the TLR signaling pathway to eliminate intracellular pathogens in innate immune system. However, it is unknown if other pathways regulate during immunologic response. Given critical role of purinergic P2X7 receptor (P2X7R) various functions (i.e., caspase activation and IL-1beta secretion), principal objective here was determine whether P2X7R may cells. We observed both MG6 mouse microglial cells primary microglia that by ATP increases expression...
Angiopoietin-1 (Ang1) regulates both vascular quiescence and angiogenesis through the receptor tyrosine kinase Tie2. We another group previously showed that Ang1 Tie2 form distinct signaling complexes at cell-cell cell-matrix contacts. further demonstrated former up-regulates Notch ligand delta-like 4 (Dll4) only in presence of Because Dll4/Notch signal restricts sprouting promotes stabilization, we investigated mechanism how Ang1/Tie2 induces Dll4 expression to clarify role signal-mediated...
Prion diseases are fatal neurodegenerative disorders and no effective treatment has been established to date. In this study, we evaluated the effect of FK506 (tacrolimus), a macrolide that is known be mild immunosuppressant, on prion infection, using cell culture animal models. We found markedly reduced abnormal form protein (PRNPSc) in cultures (N2a58 MG20) infected with Fukuoka-1 prion. The levels autophagy-related molecules such as LC3-II, ATG12–ATG5 ATG7 were significantly increased...
Abstract African swine fever virus (ASFV) is the etiological agent of (ASF), a fatal hemorrhagic disease domestic pigs and wild boar. The primarily infects macrophage monocyte host cells, these do not grow in vitro. Many attempts have been made to establish sustainable ASFV-sensitive cell lines, but which supported only low viral replication levels limited, mostly artificially attenuated strains ASFV. Here, we examined competence novel line immortalized porcine kidney macrophages (IPKM) for...
Recent studies have shown that the neurodegenerative process in disorders with Lewy body formation, such as Parkinson's disease and dementia bodies, is associated α-synuclein accumulation β-synuclein might protect central nervous system from neurotoxic effects of α-synuclein. However, mechanisms are unclear. The main objective present study was to investigate potential involvement serine threonine kinase Akt (also known protein B) signaling pathway neuroprotection. For this purpose, activity...
The discovery of α-synuclein (αS) mutations has made a major contribution to the understanding pathogenesis α-synucleinopathies such as Parkinson's disease and dementia with Lewy bodies (DLB). In contrast, less attention been paid β-synuclein (βS) mutations. this paper, we show that transgenic (tg) mice expressing DLB-linked P123H βS develop progressive neurodegeneration, characterized by axonal swelling, astrogliosis behavioural abnormalities, memory disorder being more prominent than motor...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key glycolytic enzyme that predominantly localized in the cytoplasm. However, recent studies have suggested GAPDH released by various cells and extracellular involved regulation of neuritogenesis neuronal cells. It has also been reported expressed on surfaces macrophages functions as transferrin receptor. since leaderless protein mechanisms which it reaches environment remain unclear. Here, we examined role P2X7 receptor (P2X7R), an...
Abstract α‐Synuclein accumulation plays an important role in the pathogenesis of Lewy body disease (LBD) and Parkinson's (PD). Although mechanisms are not yet clear, it is possible that dysregulation extracellular signal‐regulated kinase (ERK) might play a role. As caveolins form scaffolds onto which signaling molecules such as ERK can assemble, we propose alterations associated with α‐synuclein neurodegeneration, be mediated via caveolae. Therefore, objective present study was to...