A5083551523- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Sphingolipid Metabolism and Signaling
- Stress Responses and Cortisol
- Adipose Tissue and Metabolism
- Neuroendocrine regulation and behavior
- Connexins and lens biology
- Calpain Protease Function and Regulation
- Lipid Membrane Structure and Behavior
- Regulation of Appetite and Obesity
- Nitric Oxide and Endothelin Effects
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Nicotinic Acetylcholine Receptors Study
- Cardiac Ischemia and Reperfusion
- Biochemical Analysis and Sensing Techniques
- Tryptophan and brain disorders
- Muscle Physiology and Disorders
- Neuroscience and Neural Engineering
- Vagus Nerve Stimulation Research
- Cell Adhesion Molecules Research
- Cardiac electrophysiology and arrhythmias
Nippon Medical School
2008-2023
Showa University
2004-2015
Showa Pharmaceutical University
2001-2013
Ryukoku University
2011
Tokyo Metropolitan Institute of Medical Science
2011
Pharmac
1999
University of North Carolina at Chapel Hill
1994-1998
University of Tsukuba
1993
Growth hormone secretagogues (GHSs) stimulate GH secretion and food intake. GHS receptor (GHS-R) mRNA has been identified mainly in the arcuate nucleus (Arc) ventromedial of hypothalamus pituitary. Ghrelin, an endogenous ligand for GHS-R, recently purified from rat stomach. Although ghrelin is also expressed hypothalamus, physiological significance ghrelin/GHS-R system still unknown. We have created transgenic (Tg) rats expressing antisense GHS-R under control promoter tyrosine hydroxylase...
Growth hormone secretagogues (GHSs) stimulate GH secretion and food intake. GHS receptor (GHS-R) mRNA has been identified mainly in the arcuate nucleus (Arc) ventromedial of hypothalamus pituitary. Ghrelin, an endogenous ligand for GHS-R, recently purified from rat stomach. Although ghrelin is also expressed hypothalamus, physiological significance ghrelin/GHS-R system still unknown. We have created transgenic (Tg) rats expressing antisense GHS-R under control promoter tyrosine hydroxylase...
The involvement of microglia in neuroinflammatory responses has been extensively demonstrated. Recent animal studies have shown that exposure to either acute or chronic stress induces robust microglial activation the brain. In present study, we investigated underlying mechanism brain by stress.We first looked at spatial distribution noradrenaline (NA)-synthesizing enzyme, DBH (dopamine β-hydroxylase), comparison with NA receptors-β1, β2, and β3 adrenergic receptors (β1-AR, β2-AR,...
Although dysfunction of VE-cadherin-mediated adherence junctions in vascular endothelial cells (ECs) is thought to be one the initial steps atherosclerosis, little known regarding how VE-cadherin disrupted during atherogenic development. This study focused on role calpain, an intracellular cysteine protease, proteolytic disorganization and subsequent progression atherosclerosis.Increased expression m-calpain was observed aortic ECs atherosclerotic lesions humans low-density lipoprotein...
Mitochondrial free Ca 2+ may regulate mitochondrial ATP production during cardiac exercise. Here, using laser scanning confocal microscopy of adult rabbit myocytes co‐loaded with Fluo‐3 to measure and tetramethylrhodamine methylester identify mitochondria, we measured cytosolic transients the contractile cycle. In resting cells, signals were similar. During electrical pacing, fluorescence occurred in both compartments. Both potentiated by isoproterenol. These experiments show directly that...
To clarify the role of ghrelin in regulatory mechanism energy metabolism, we analyzed effects centrally and peripherally administered on noradrenaline release brown adipose tissue (BAT) rats using a microdialysis system. I.c.v. administration at dose 500 pmol suppressed BAT, microinjection (50 pmol) into paraventricular nucleus (PVN) or arcuate (ARC) hypothalamus also BAT. In addition, i.v. (30 nmol) this suppression was blocked by vagotomy. Neither i.c.v. nor des-acyl ghrelin, which does...
Thrombolysis therapy using tissue-type plasminogen activator (t-PA) is occasionally accompanied by harmful outcomes, including intracerebral hemorrhage. We have reported that Stachybotrys microspora triprenyl phenol-7 (SMTP-7), a candidate thrombolytic drug, has excellent therapeutic effect on cerebral infarction in embolic stroke model mice; however, little known regarding whether this agent influences cerebrovascular inflammation following reperfusion. The current study aimed to compare...
The present investigation was conducted in order to determine whether lysophosphatidic acid (LPA) induces itch-scratch responses (ISRs) mice. Intradermal administration of LPA ISRs; furthermore, the time course for LPA-induced ISRs similar that histamine-induced responses. Comparative study pruritogenic activity revealed histamine possessed a potent effect characterized by dose-response relationship; however, prostaglandin D2 failed induce this response. Pretreatment with ketotifen, H1...
The mechanisms for mobilization of intracellular free Ca2+ have been studied in various types isolated and cultured cells, but little is known about individual cells situ. We tried to establish imaging analysis concentration ([Ca2+]i) loaded with the acetoxymethyl ester fluo 3 situ, using laser scanning confocal microscopy. method permitted us distinguish signals from endothelial smooth muscle guinea pig artery. Addition ATP artery caused a transient increase [Ca2+]i. It was concluded that...
Proteolytic activity in sheared human umbilical vein endothelial cells (HUVECs) was measured using a fluorogenic substrate and laser scanning confocal microscopy to clarify the key role of an intracellular Ca(2+)-sensitive protease, calpain, these response shear stress. Within physiological range, enhanced shear-dependent fashion. Short interfering RNA-induced silencing m-calpain, but not micro-calpain, suppressed activity. Either removal extracellular Ca(2+) or application chelator...
The biological basis for the therapeutic mechanisms of depression is still unknown. We have previously performed expressed-sequence tag (EST) analysis to identify some molecular machinery responsible antidepressant effect. Then, we developed our original cDNA microarray, on which fragments identified as antidepressant-related genes/ESTs were spotted. In this study, with microarray followed by Western blot analysis, demonstrated induction vesicle-associated membrane protein...
Growth hormone secretagogues (GHSs) stimulate GH secretion and food intake. GHS receptor (GHS-R) mRNA has been identified mainly in the arcuate nucleus (Arc) ventromedial of hypothalamus pituitary. Ghrelin, an endogenous ligand for GHS-R, recently purified from rat stomach. Although ghrelin is also expressed hypothalamus, physiological significance ghrelin/GHS-R system still unknown. We have created transgenic (Tg) rats expressing antisense GHS-R under control promoter tyrosine hydroxylase...