A5031219114- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Animal Nutrition and Physiology
- Veterinary medicine and infectious diseases
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Helminth infection and control
- Amoebic Infections and Treatments
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Clostridium difficile and Clostridium perfringens research
- Microbial infections and disease research
- Veterinary Oncology Research
- Bird parasitology and diseases
- Listeria monocytogenes in Food Safety
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Meat and Animal Product Quality
- Parasites and Host Interactions
- Nail Diseases and Treatments
- Parasitic infections in humans and animals
- Congenital Diaphragmatic Hernia Studies
- Ichthyology and Marine Biology
Osaka Prefecture University
2013-2024
Osaka Metropolitan University
2022-2024
Tokyo Metropolitan University
2023-2024
John Wiley & Sons (United States)
2020
Moss Landing Marine Laboratories
2020
Hudson Institute
2020
University of Veterinary Science
2016
Indian Veterinary Research Institute
2016
University of Veterinary Medicine
2015
Council for Science, Technology and Innovation
2015
The abilities of Salmonella serovars to colonize the reproductive organs chickens and contaminate eggs were compared. Mature laying hens inoculated intravenously with 10(5) colony-forming units enteritidis, typhimurium, infantis, hadar, heidelberg, or montevideo cause systemic infection. enteritidis was recovered from three yolks laid (7.0%), suggesting egg contamination transovarian transmission S. enteritidis. liver, spleen, cecum colonized by each serovar similarly at 4 7 days...
Laying hens were inoculated intravaginally (IVg) once (IVg-single) or three times (IVg-triple), intracloacally (IC), intravenously (IV) with Salmonella enteritidis (SE) phage type 4. Eggs tested significantly (P < 0.05) fewer positive in group IC than other groups. SE was recovered from egg contents the groups IVg-single (9.6%), IVg-triple (4.2%), and IV (11.5%). IVg inoculation resulted colonization of cloaca lower portions oviduct but not portion above isthmus, whereas entire oviduct. Only...
The effects of different levels oocyst inocula Eimeria acervulina, E. tenella, or maxima on plasma carotenoids, total lipids, proteins, packed cell volume (PCV), bird performance, and coccidial lesion scores were determined in male Hubbard chickens. Each test consisted an uninfected treatment either four (E. tenella maxima) five acervulina) inocula. Carotenoids lipids significantly (P < = 0.05) depressed by acervulina starting at 10(2) oocysts per bird, whereas 10(4) higher inoculum protein....
In Experiment 1, mature laying hens were inoculated intravaginally with 10(6) colony-forming units of Salmonella enterica serovar enteritidis (S. enteritidis), typhimurium, infantis, hadar, heidelberg, or montevideo to compare their abilities colonize the reproductive organs chickens and contaminate eggs. was more frequently recovered (from 11 40 eggs, 27.5%) than other serovars, especially inner shell contaminated these organisms in 10 eggs (25.0%). The contamination rates viable counts...
The prevalence of Salmonella in four layer farms eastern Japan was investigated between 2004 and 2006 to determine the role roof rats (Rattus rattus) epizootology enterica subsp. serovar Enteritidis (S. Enteritidis). Persistent S. Infantis contamination environment pooled egg samples were detected three out farms. A total 113 (13.3%) 158 (18.6%) 851 examined positive for Infantis, respectively. By pulsed-field gel electrophoresis, only one indistinguishable pattern yielded by strains from...
The phylum Apicomplexa comprises obligate intracellular parasites that infect vertebrates. All invasive forms of possess an apical complex, a unique assembly organelles localized to the anterior end cell and involved in host invasion. Previously, we generated chicken monoclonal antibody (mAb), 6D-12-G10, with specificity for antigen located cytoskeleton Eimeria acervulina sporozoites. This was highly conserved among Apicomplexan parasites, including other spp., Toxoplasma, Neospora,...
Molecular biological techniques have enabled the accurate identification of avian Eimeria parasite, however, preparation PCR template remains a bottleneck due to contaminants from feces and robust oocyst's wall resistant chemical mechanical force. Generally, involves three main steps: (1) pretreatment oocysts; (2) disruption (3) purification genomic DNA. We prepared templates both unsporulated sporulated E. tenella oocysts using various protocols, followed by species-specific define limit...
Eimeria tenella is an intracellular apicomplexan parasite, which infects cecal epithelial cells from chickens and causes hemorrhagic diarrhea eventual death. We have previously reported the comparative RNA sequence analysis of E. sporozoite stage between virulent precocious strains showed that expression several genes involved in mitochondrial electron transport chain (ETC), such as type II NADH dehydrogenase (NDH-2), complex (succinate:quinone oxidoreductase), malate:quinone oxidoreductase...
A total of 284 fecal samples 89 species (43 mammalian and 46 avian species) were examined for Cryptosporidium oocysts Giardia cysts from 1999 to 2002. Each sample was collected at the zoo located Osaka in Japan by microscopy after performing sucrose flotation method two immunofluorescent assay kits detection cysts. spp. found only a raccoon dog (Nyctereutes procyonoides), detected mandarin duck (Aix galericulata) ruddy shelducks (Tadorna ferruginea). In this study, prevalences these...
To characterize bone fractures and the usefulness of micro-CT for imaging in pet rabbits.Retrospective case series.210 client-owned rabbits with fractures.Medical records evaluated from 2007 through 2013 were examined. Information was collected on signalment nature fractures, radiographic images reviewed.Almost half (n = 95 [47.7%]) < 3 years old. Accidental fall most common cause. Vertebral fracture type a nonneoplastic cause 46 [23.2%]) L4-L7 region. The tibia site limb among all (45...
OBJECTIVE To evaluate outcome of limb fracture repair in rabbits. DESIGN Retrospective case series. ANIMALS 139 client-owned rabbits with fractures treated between 2007 and 2015. PROCEDURES Medical records were reviewed for information on location, treatment, time to healing. RESULTS 25 had involving the distal aspects limbs (ie, metacarpal or metatarsal bones, phalanges, calcaneus talus). Fractures 23 these (external coaptation, n = 17; external skeletal fixation, 4; intramedullary pinning,...
An epidemiological investigation of 120 canine otitis externa cases in 1,370 dogs was done on the incidence rate, ear pinna shapes, breeds and their relationships. Eighty-five (12.6%) 672 with pendulous ears 35 (5.0%) 698 erect had externa, difference between them significant (P<0.05). Ninety-five auditory cerumen specimens were cultured for Malassezia pachydermatis (M. pachydermatis) analyzed concentrations major fatty acids. Although rates positive M. both shapes almost same, i.e. 55.2%...
Salmonella enterica serovar Enteritidis (SE) is a causative agent for human food poisoning cases throughout the world. The ovaries and oviducts of laying hens are major sites SE colonization from which vertical transmission to eggs occurs. In this study, Salmonella-induced changes in T lymphocytes, B lymphocytes macrophages were assessed after primary secondary experimental inoculations hen with SE. Statistically significant increases numbers cells (both CD4+ CD8+) observed 7 14 days...