A5060441431- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
- Trypanosoma species research and implications
- Bipolar Disorder and Treatment
- Enzyme Structure and Function
- Research on Leishmaniasis Studies
- Crystallization and Solubility Studies
- Cancer Research and Treatments
- X-ray Diffraction in Crystallography
- Malaria Research and Control
- Surfactants and Colloidal Systems
- Crystal structures of chemical compounds
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- Photosynthetic Processes and Mechanisms
- Analytical Chemistry and Chromatography
- Porphyrin Metabolism and Disorders
- ATP Synthase and ATPases Research
- Protein Structure and Dynamics
- Magnetism in coordination complexes
- Parasitic Infections and Diagnostics
- Metal-Catalyzed Oxygenation Mechanisms
- Lysosomal Storage Disorders Research
- Thermodynamic properties of mixtures
- Mosquito-borne diseases and control
Kyoto Institute of Technology
2012-2021
University of Shizuoka
2004-2018
Osaka University
1986-2017
The University of Tokyo
1996-2013
Juntendo University
2008-2013
de Duve Institute
2013
Ahmadu Bello University
2013
Yamaguchi University
2009
Kitasato University
2009
Nagasaki University
2009
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and molecular structure of [7]circuleneKoji. Yamamoto, Tadashi. Harada, Yoshio. Okamoto, Hiroaki. Chikamatsu, Masao. Nakazaki, Yasushi. Kai, Takuo. Nakao, Mitsuya. Tanaka, Shigeharu. Nobutami. KasaiCite this: J. Am. Chem. Soc. 1988, 110, 11, 3578–3584Publication Date (Print):May 1, 1988Publication History Published online1 May 2002Published inissue 1 1988https://doi.org/10.1021/ja00219a036RIGHTS & PERMISSIONSArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and characterization of [7]circuleneKoji Yamamoto, Tadashi Harada, Masao Nakazaki, Takuo Naka, Yasushi Kai, Shigeharu Nobutami KasaiCite this: J. Am. Chem. Soc. 1983, 105, 24, 7171–7172Publication Date (Print):November 1, 1983Publication History Published online1 May 2002Published inissue 1 November 1983https://pubs.acs.org/doi/10.1021/ja00362a025https://doi.org/10.1021/ja00362a025research-articleACS PublicationsRequest reuse...
In addition to haem copper oxidases, all higher plants, some algae, yeasts, molds, metazoans, and pathogenic microorganisms such as Trypanosoma brucei contain an additional terminal oxidase, the cyanide-insensitive alternative oxidase (AOX). AOX is a diiron carboxylate protein that catalyzes four-electron reduction of dioxygen water by ubiquinol. T. brucei, parasite causes human African sleeping sickness, plays critical role in survival its bloodstream form. Because absent from mammals, this...
Recent studies on the respiratory chain of Ascaris suum showed that mitochondrial NADH-fumarate reductase system composed complex I, rhodoquinone and II plays an important role in anaerobic energy metabolism adult A. suum. The is major pathway for adaptation to a hypoxic environment not only parasitic organisms, but also some types human cancer cells. Thus, enzymes are potential targets chemotherapy. We found flutolanil excellent inhibitor (IC50 = 0.058 μM) less effectively inhibits...
NADPH oxidase produces reactive oxygen species (ROS). Drosophila melanogaster has two homologs of oxidase, dNox and dDuox, with functions that remain unclear in vivo. To clarify these functions, independent transgenic fly lines expressing dsRNA targeted for different portions dDuox mRNA were used. In both flies, en-GAL4> UAS-dDuoxIR(976-1145) UAS-dDuoxIR(370-518), which was knocked down selectively the posterior area wing disc, compartment adult wings became paler more fragile veins...
Background Chagas disease, caused by the parasite Trypanosoma cruzi, is a neglected tropical disease that causes severe human health problems. To develop new chemotherapeutic agent for treatment of we predicted pharmacophore model T. cruzi dihydroorotate dehydrogenase (TcDHODH) fragment molecular orbital (FMO) calculation orotate, oxonate, and 43 orotate derivatives. Methodology/Principal Findings Intermolecular interactions in complexes TcDHODH with derivatives were analyzed FMO at...
Human dihydroorotate dehydrogenase (HsDHODH) is a key enzyme of pyrimidine de novo biosynthesis pathway. It located on the mitochondrial inner membrane and contributes to respiratory chain by shuttling electrons ubiquinone pool. We have discovered ascofuranone (1), natural compound produced Acremonium sclerotigenum, its derivatives are potent class HsDHODH inhibitors. conducted structure-activity relationship study identified functional groups 1 that essential for inhibition enzymatic...
Malaria is one of the three major global health threats. Drug development for malaria, especially its most dangerous form caused by Plasmodium falciparum, remains an urgent task due to emerging drug-resistant parasites. Exploration novel antimalarial drug targets identified a trifunctional enzyme, malate quinone oxidoreductase (MQO), located in mitochondrial inner membrane P. falciparum (PfMQO). PfMQO involved pathways electron transport chain, tricarboxylic acid cycle, and fumarate cycle....
ABSTRACT In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a found in the aklavinone-aclacinomycin cluster Streptomyces galilaeus . order to identify its function, was expressed Escherichia coli The cell extract prepared from E. cells overexpressing AknX protein exhibited anthrone oxygenase activity, which converted emodinanthrone anthraquinone emodin. This indicates that and...
Current assays for screening new antimalarials need initiators of beta-hematin formation that require laborious preparation, special devices, and substrates. In this study, based on reduction heme absorption in formation, we developed a simple colorimetric assay using Tween 20 as an initiator microplate reader high-throughput inhibitors formation.
Dihydroorotate dehydrogenase (DHOD) from Trypanosoma cruzi (TcDHOD) is a member of family 1A DHOD that catalyzes the oxidation dihydroorotate to orotate (first half-reaction) and then reduction fumarate succinate (second in de novo pyrimidine biosynthesis pathway. The coupled with FMN, reduced FMN converts second half-reaction. TcDHOD are known be essential for survival growth T. validated drug target. first-half reaction mechanism Lactococcus lactis has been extensively investigated on...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnthalpic studies on the state of water in sodium bis(2-ethylhexyl)sulfosuccinate reversed micellesAyako Goto, Shigeharu Harada, Teruyuki Fujita, Youko Miwa, Hisashi Yoshioka, and Hiroshi KishimotoCite this: Langmuir 1993, 9, 1, 86–89Publication Date (Print):January 1993Publication History Published online1 May 2002Published inissue 1 January 1993https://pubs.acs.org/doi/10.1021/la00025a020https://doi.org/10.1021/la00025a020research-articleACS...
Journal Article Siccanin Rediscovered as a Species-Selective Succinate Dehydrogenase Inhibitor Get access Tatsushi Mogi, Mogi * 1Department of Biomedical Chemistry, the University Tokyo, Bunkyo-ku, Tokyo 113-0033; 2Department Biotechnology, 113-8657; 3Department Biological Faculty Agriculture, Yamaguchi University, 753-8515; 4Kitasato Institute for Life Sciences and Graduate School Infection Control Sciences, Kitasato Minato-ku, 108-8641; 5Department Applied Biology, Kyoto Technology,...
Methionine γ‐lyase (MGL) (EC 4.4.1.11), which is present in certain lineages of bacteria, plants, and protozoa but missing mammals, catalyzes the single‐step degradation sulfur‐containing amino acids (SAAs) to α‐keto acids, ammonia, thiol compounds. In contrast other organisms possessing MGL, anaerobic parasitic protists, namely Entamoeba histolytica Trichomonas vaginalis , harbor a pair MGL isozymes. The enteric protozoon En. shows various unique aspects its metabolism, particularly SAAs....
In the anaerobic respiratory chain of parasitic nematode Ascaris suum, complex II couples reduction fumarate to oxidation rhodoquinol, a reverse reaction catalyzed by mammalian II. this study, first structure mitochondria was determined. The structure, composed four subunits and five co-factors, is similar that aerobic II, except for an extra peptide found in smallest anchor subunit A. suum enzyme. We discuss herein structure-function relationship enzyme critical role low redox potential rhodoquinol