A5038461246- X-ray Diffraction in Crystallography
- Enzyme Structure and Function
- Ubiquitin and proteasome pathways
- Crystallization and Solubility Studies
- Endoplasmic Reticulum Stress and Disease
- Phase Equilibria and Thermodynamics
- Bacterial Genetics and Biotechnology
- Peptidase Inhibition and Analysis
- High-pressure geophysics and materials
- Vibrio bacteria research studies
- Glycosylation and Glycoproteins Research
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Face recognition and analysis
- 3D Shape Modeling and Analysis
- Atomic and Molecular Physics
- Mass Spectrometry Techniques and Applications
- Machine Learning in Materials Science
- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Physics Studies
- Medical Imaging Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- nanoparticles nucleation surface interactions
- Microbial Metabolism and Applications
- Corporate Governance and Management
TU Dresden
2022
Society for Chemical Engineering and Biotechnology
2003-2022
Tris Pharma (United States)
2021
University of Basel
2013-2020
Imperial College London
2008-2019
Otto-von-Guericke University Magdeburg
2017
Max Planck Institute for Nuclear Physics
2016
Barco (Belgium)
2014
Centre for Structural Systems Biology
2014
Centre National de la Recherche Scientifique
2013
The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. new EIGER detector features pixel size 75 × µm, frame rates up to 3000 Hz dead time low 3.8 µs. An 1M 16M were tested on Swiss Light Source beamlines X10SA X06SA for their application crystallography. combination fast very short allows high-quality data acquisition shorter time....
The type VI secretion system (T6SS) is crucial in interbacterial competition and a virulence determinant of many Gram-negative bacteria. Several T6SS effectors are covalently fused to secreted structural components such as the VgrG spike for delivery into target cells. In Pseudomonas aeruginosa, VgrG2b effector was previously proposed mediate bacterial internalization eukaryotic this work, we find that C-terminal domain (VgrG2bC-ter) elicits toxicity periplasm, counteracted by cognate...
After host cell entry, Salmonella replicate in membrane-bound compartments, which accumulate a dense meshwork of F-actin through the kinase activity SPI-2 type III secretion effector SteC. We find that SteC promotes actin cytoskeleton reorganization by activating signaling pathway involving MAP kinases MEK and ERK, myosin light chain (MLCK) Myosin IIB. Specifically, phosphorylates directly on serine 200 (S200), previously unstudied phosphorylation site. S200 is predicted to displace negative...
ABSTRACT Respiratory syncytial virus (RSV) infects epithelial cells of the respiratory tract and is a major cause bronchiolitis pneumonia in children elderly. The assembles buds through plasma membrane, forming elongated membrane filaments, but details how this happens remain obscure. Oligomerization matrix protein (M) key step process assembly infectious production. In addition, it was suggested to affect conformation fusion protein, current target for RSV antivirals, mature virus....
Hybrid photon counting (HPC) detectors have radically transformed basic research at synchrotron light sources since 2006. They excel X-ray diffraction applications in the energy range from 2 to 100 keV. The main reasons for their superiority are direct detection of individual photons and accurate determination scattering intensities over an extremely high dynamic range. were first adopted macromolecular crystallography where they revolutionized data collection. soon also used small-angle...
The type VI secretion system (T6SS) is a bacterial nanomachine for the transport of effector molecules into prokaryotic and eukaryotic cells. It involves assembly tubular structure composed TssB TssC that similar to tail sheath bacteriophages. contracts provide energy needed delivery. AAA(+) ATPase ClpV disassembles contracted sheath, which resets systems reassembly an extended ready fire again. This mechanism crucial T6SS function. In Vibrio cholerae, binds N terminus within hydrophobic...
p97 is a key regulator of numerous cellular pathways and associates with ubiquitin-binding adaptors to remodel ubiquitin-modified substrate proteins. How adaptor binding coordinated how contribute remodeling unclear. Here we present the 3D electron cryomicroscopy reconstructions major Ufd1-Npl4 in complex p97. Our show that p97-Ufd1-Npl4 highly dynamic assumes distinct positions relative ring upon addition nucleotide. results suggest model for by also explains could form other complexes...
The type III secretion system is a widespread apparatus used by pathogenic bacteria to inject effectors directly into the cytoplasm of eukaryotic cells. A key component this highly conserved translocon, pore formed in host membrane that essential for toxins bypass last physical barrier. In Pseudomonas aeruginosa translocon composed PopB and PopD, both which before are stabilized within bacterial common chaperone, PcrH. work we characterize PopB, major translocator, membrane-associated...
The plasma composition, equation of state, and phase diagram dense helium were calculated for temperatures 10 4 ...10 5 K, total atom densities 15 ... 25 cm− 3 , pressures up to 2 TPa, including the region partial ionization strong Coulomb coupling. basic thermodynamic potential was chosen be free energy density with contributions due interaction, hard-core repulsion, van der Waals-like attraction a mixture differently charged atoms electrons. For first time, we show occurrence sequence...
The type II AAA+ protein p97 is involved in numerous cellular activities, including endoplasmic reticulum-associated degradation, transcription activation, membrane fusion and cell-cycle control. These activities are at least part regulated by the ubiquitin system, which thought to target ubiquitylated substrates within macromolecular complexes assist their extraction or disassembly. Although ATPase activity essential for function, little known about how ATP binding hydrolysis coupled with...
Macromolecular crystallography (MX) is the dominant means of determining three-dimensional structures biological macromolecules, but method has reached a critical juncture. New diffraction-limited storage rings and upgrades to existing sources will provide beamlines with higher flux brilliance, even largest detectors can collect at rates several hundred hertz. Electron cryomicroscopy successfully competing for structural biologists' most exciting projects. As result, formerly scarce beam...
Macromolecular crystallography (MX) is the dominant means of determining three-dimensional structures biological macromolecules. Over last few decades, most MX data have been collected at synchrotron beamlines using a large number different detectors produced by various manufacturers and taking advantage protocols goniometries. These came in their own formats: sometimes proprietary, open. The associated metadata rarely reached degree completeness required for management according to...