A5071432395- Electrospun Nanofibers in Biomedical Applications
- Organ and Tissue Transplantation Research
- Tissue Engineering and Regenerative Medicine
- Infectious Aortic and Vascular Conditions
- Vascular Procedures and Complications
- Peripheral Artery Disease Management
- Reconstructive Surgery and Microvascular Techniques
- Renal and Vascular Pathologies
- Diabetic Foot Ulcer Assessment and Management
- Bone Tissue Engineering Materials
- Cardiac and Coronary Surgery Techniques
- Organ Donation and Transplantation
- Aortic aneurysm repair treatments
- Polymer Surface Interaction Studies
- Venous Thromboembolism Diagnosis and Management
- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Abdominal vascular conditions and treatments
- Cardiac tumors and thrombi
- Acute Ischemic Stroke Management
- Vascular anomalies and interventions
- Renal cell carcinoma treatment
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Pediatric Hepatobiliary Diseases and Treatments
Institute of Clinical and Experimental Medicine
2013-2025
Charles University
2018-2025
Czech Academy of Sciences
2008-2024
Czech Academy of Sciences, Institute of Physiology
2009-2024
NHS Blood and Transplant
2020
Czech Technical University in Prague
2013
Cardiovascular Research Center
2009
University of Veterinary Science
1980
Introduction: Uterus transplantation (UTx) is a rapidly evolving treatment of uterine-factor infertility. We report the results first 10 UTx procedures performed at our institution. Methods: The program started in April 2016 as two-arm study comparing efficacy from live donors (LD) and deceased (DD). Results: Between 2018, we five DD LD UTx. Two grafts had to be removed early due thrombosis. One graft was chronic rejection previous herpes simplex infection month 7. Graft survival 70% one...
Abstract Currently used vascular prostheses are hydrophobic and do not allow endothelial cell (EC) adhesion growth. The aim of this study was to prepare fibrin (Fb)‐based two‐dimensional (2D) three‐dimensional (3D) assemblies coated with extracellular matrix (ECM) proteins evaluate the EC adhesion, proliferation differentiation on these in vitro . Coating Fb collagen, laminin (LM), fibronectin (FN) proved using surface plasmon resonance technique. On all assemblies, ECs reached higher...
Background. Nulliparous uterine grafts have never been used in uterus transplantation (UTx), possibly due to presumed infertility. Our objective was verify the feasibility of nulliparous graft transplantation. Methods. The Czech Uterus Transplant Trial (registered under ClinicalTrials.gov, identifier NCT03277430) is a 2-arm trial comparing efficacy deceased donor (DD) versus live-donor transplant (10 patients both arms). A 25-year-old patient suffering from inborn absolute factor infertility...
Background: Cardiovascular surgery is confronted by a lack of suitable materials for patch repair. Acellular animal tissues serve as an abundant source promising biomaterials. The aim our study was to explore the bio-integration decellularized or recellularized pericardial matrices in vivo. Methods: Porcine (allograft) and ovine (heterograft, xenograft) pericardia were using 1% sodium dodecyl sulfate ((1) Allo-decel (2) Xeno-decel). We used two cell types pressure-stimulated...
Abstract Various techniques for coating synthetic surfaces with fibrin structures were tested seeding bovine pulmonary artery endothelial cells (EC). Two‐dimensional (Fb) (2D Fb) obtained by successively repeating adsorption of fibrinogen (Fbg), incubating the surface thrombin (Thr) solution, and inhibiting surface‐attached Thr. Three‐dimensional networks immobilized at (3D formed catalytic action on an ambient Fbg solution. Ultra‐thin 3D Fbs if inhibitors antithrombin III heparin added into...
Cardiovascular prosthetic bypass grafts do not endothelialize spontaneously in humans, and so they pose a thrombotic risk. Seeding with cells improves their performance, particularly small-caliber applications. Knitted tubular polyethylene-terephthalate (PET) vascular prostheses (6 mm) commercial type I collagen (PET/Co) were modified the lumen by adsorption of laminin (LM), coating fibrin network (Fb) or combination Fb fibronectin (Fb/FN). Primary human saphenous vein endothelial seeded...
Abstract Recombinant interferon-α (IFN-α) treatment functionally cures chronic hepatitis B virus (HBV) infection in some individuals and suppresses replication hepatocytes infected vitro. We studied the antiviral effect of conditioned media (CM) from peripheral blood mononuclear cells (PBMCs) stimulated with agonists Toll-like receptors (TLRs) 2, 7, 8 9. found that CM PBMCs dual-acting TLR7/8 (R848) TLR2/7 (CL413) were more potent drivers inhibition HBe HBs antigen secretion HBV-infected...
Vascular surgery for atherosclerosis is confronted by the lack of a suitable bypass material. Tissue engineering strives to produce bio-artificial conduits provide resistance thrombosis. The objectives our study were culture endothelial cells (EC) on composite assemblies extracellular matrix proteins, and evaluate cellular phenotype under flow. Cell-adhesive fabricated glass slides as combinations collagen (Co), laminin (LM), fibronectin (FN), resulting in three samples: Co, Co/LM, Co/FN....
(1) Background: Decellularized xenogeneic tissues are promising matrices for developing tissue-engineered cardiovascular grafts. In vitro recellularization of these with stromal cells can provide a better in vivo remodelling and lower thrombogenicity the graft. The process be accelerated using cultivation bioreactor simulating physiological conditions stimuli. (2) Methods: Porcine pericardium was decellularized custom-built decellularization system an optimized protocol. Autologous porcine...
Highlights•Endovascular treatment of isolated iliac artery dissection (ISIAD) is a viable modality.•It has very low peri and post- procedural complications.•Due to its mini invasiveness it the preferred choice over open repair.AbstractIntroductionIsolated without involvement aorta rare medical condition.ReportA case 38-year-old man with sudden onset rest pain paraesthesia on right lower limb (RLL) presented. Upon admission, RLL was pulseless, mild in foot. The patient underwent computed...
Abstract Aim To assess the impact of autologous cell therapy on critical limb ischaemia in people with diabetes and diabetic kidney disease. Methods A total 59 (type 1 or type 2) ischaemia, persisting after standard revascularization, were treated our foot clinic over 7 years; this group comprised 17 42 without severe The control had same inclusion criteria, but was conservatively 21 23 Severe disease defined as chronic stages 4–5 ( GFR <30 ml/min/1.73 m²). Death amputation‐free survival...
Kudla M operated on the patient.Chlupac J and Novotny R co-analyzed data provided consultations regarding risk of thrombosis
We report the case of a 43-year-old female patient with systemic lupus erythematosus, class III nephritis, predialysis creatinine levels around 350 μmol/L (3.95 mg/dL) after partial resection left kidney histologically verified papillary carcinoma in 2010. Preoperative computed tomography abdomen revealed small 8 mm tumor upper pole. The was indicated for simultaneous bilateral nephrectomy and orthotopic renal transplantation aim to minimize invasiveness procedure as well curable removal.The...
Uterus transplantation is a treatment solution for women suffering from absolute uterine factor infertility. As much as 19.5% of uterus-transplanted patients underwent urgent graft hysterectomy due to thrombosis/hypoperfusion. The necessity identify candidates with high-quality vasculature paramount. We retrospectively evaluated and compared the imaging results actual vascular findings back table. In this article, we present novel radiologic grading scale (DUETS classification) evaluating...
A 27-year-old female patient with known tuberous sclerosis complex (TSC), polycystic kidneys multiple large bilateral angiomyolipomas, and failing renal functions prehemodialysis values (urea: 19 mmol/L; creatinine: 317 μmol/L; CKD-EPI 0,27) was admitted to our department for pre-renal transplant evaluation. The placed on the waiting list as living donor did not pass pretransplant workup subsequently contraindicated. Patient "cadaverous kidney list". Computed tomography angiography revealed...
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