A5041033799- Natural Antidiabetic Agents Studies
- Pancreatic function and diabetes
- Advanced Glycation End Products research
- PARP inhibition in cancer therapy
- Biochemical Acid Research Studies
- Fungal Biology and Applications
- Environmental Toxicology and Ecotoxicology
- Diet, Metabolism, and Disease
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Effects of Radiation Exposure
- Phytochemicals and Antioxidant Activities
- RNA Interference and Gene Delivery
- Alcoholism and Thiamine Deficiency
- Curcumin's Biomedical Applications
- Diabetes and associated disorders
- Cytokine Signaling Pathways and Interactions
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Tannin, Tannase and Anticancer Activities
- Nuclear Structure and Function
- Metabolism, Diabetes, and Cancer
- Endoplasmic Reticulum Stress and Disease
- DNA Repair Mechanisms
University of Belgrade
2015-2025
Institute for Biological Research “Siniša Stanković”
2006-2022
Institute for Biological Sciences
2007-2019
Maize Research Institute Zemun Polje
2005
MRC Laboratory of Molecular Biology
2003
Diabetic foot ulcers have an enormous impact on patients’ quality of life and represent a major economic burden. The cause is delayed incomplete wound healing due to hyperglycemia, reduced blood flow, infections, oxidative stress chronic inflammation. Plasma-activated water (PAW) emerging as new therapeutic approach in treatment, it has many the advantages cold atmospheric plasma but easier apply, thus allowing for widespread use. aim this study was investigate potential PAW improve diabetic...
Due to intrinsically low levels of antioxidant enzyme expression and activity, insulin producing pancreatic β-cells are particularly susceptible free radical attack. In diabetes mellitus, which is accompanied by high oxidative stress, this feature significantly contributes their damage dysfunction. light the documented pro-survival effect chemokine C-X-C Ligand 12 (CXCL12) on β-cells, we examined its potential role in protection. We report that CXCL12 overexpression enhanced resistance rat...
The aim of this study was to assess the in vivo effects extract medicinal mushroom, Lactarius deterrimus, when administered (60 mg/kg, i.p.) daily for four weeks streptozotocin- (STZ-) induced diabetic rats. Diabetic rats treated with L. deterrimus displayed several improved biochemical parameters circulation: reduced hyperglycemia, lower triglyceride concentration and glycated hemoglobin, serum protein, advanced glycation end product (AGE) levels. This treatment also adjusted...
The present study aimed to investigate the effects of treatment with α-lipoic acid (LA), a naturally occurring compound possessing antioxidant activity, on liver oxidant stress in rat model streptozotocin (STZ)-induced diabetes by examining potential mechanistic points that influence changes expression enzymes such as catalase (CAT) and CuZn/Mn superoxide dismutase(s) (SOD). LA was administered for 4 weeks daily intraperitoneal injections (10 mg/kg) STZ-induced diabetic rats, starting from...
Chlamydia trachomatis (Ct) can induce scarring disease of the ocular mucosa, known as trachoma, most common infectious cause blindness worldwide. We hypothesized that epithelial-mesenchymal transition (EMT) contributes to fibrotic process in trachomatous scarring. Infection human conjunctival epithelial cells (HCjE) with Ct activated signaling pathways involved EMT induction, which was correlated decreased expression E-cadherin, guardian phenotype. In addition, infection associated increased...
The present study aimed to investigate the beneficial effects of treatment with extracts from edible mushroom Lactarius deterrimus (Ld) and chestnut Castanea sativa (Cs), separately in combination (MIX Ld/Cs), on oxidative stress advanced glycation end-product (AGE)-mediated hepatorenal injury a rat model streptozotocin (STZ)-induced diabetes by examining pathways responsible for maintenance redox homeostasis. An experimental was induced rats administration 40 mg/kg STZ intraperitoneally...
Pancreatic β-cell death or dysfunction mediated by oxidative stress underlies the development and progression of diabetes mellitus. In present study, we tested extracts from edible mushroom Lactarius deterrimus chestnut Castanea sativa , as well their mixture (MIX Ld / Cs ), for potential beneficial effects on streptozotocin (STZ)-induced pancreatic death. Analysis chelating effects, reducing power radical-scavenging assays revealed strong antioxidant C. extract MIX while L. displayed a weak...
Despite significant progress, the molecular mechanisms responsible for pancreatic beta cell depletion and development of diabetes remain poorly defined. At present, there is no preventive measure against diabetes. The positive impact CXCL12 expression on prosurvival phenotype initiated this study. Our aim was to provide novel insight into regulation rat gene (Cxcl12) transcription. roles poly(ADP-ribose) polymerase-1 (PARP-1) transcription factor Yin Yang 1 (YY1) in Cxcl12 were studied by...
The mechanisms underlying the beneficial effect observed in diabetic rats after treatment with a commercially available β-glucan-enriched extract (BGEE) were examined. Multiple low-dose streptozotocin (STZ) diabetes was used (40 mg STZ/kg) as model for type 1 diabetes. BGEE administered daily (80 mg/kg) 4 weeks, starting from last day of STZ treatment. In vitro and vivo experiments suggest that significant free radical scavenging antioxidant activities responsible systemic adjustment redox...
To examine the protective potential of Cotinus coggygria Scop. methanol extract, Wistar rats were treated with hepatotoxic compound pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The extract counteract stress was examined in that injected intraperitoneally (500 mg·(kg body weight) –1 ) either 2 or 12 h before pyrogallol treatment. reducing activity vitro an chelate ferrous ion both vivo vitro. Application prior treatment led decrease...
Abstract The synthesis of alpha‐2‐macroglobulin (α2M) is low in adult rat liver and elevated fetal liver. During the acute‐phase (AP) response it becomes significantly increased both In this work, cross talk STAT3 NF‐κB transcription factors during α2M gene expression was analysed. Using immunoblotting, their cellular compartmentalization examined by comparing cytoplasmic levels with active equivalents, 86 91 kDa isoforms p65‐subunit, respectively, nuclear extract matrix. Different...
Beta cell dysfunction by loss of beta identity, dedifferentiation, and the presence polyhormonal cells are main characteristics diabetes. The straightforward strategy for curing diabetes implies reestablishment pancreatic function replacement therapy. Aristaless-related homeobox (Arx) gene encodes protein which plays an important role in development alpha is a target changing identity. In this study we used CRISPR/dCas9-based epigenetic tools targeted hypermethylation Arx promoter its...