Hydrophilic interaction liquid chromatography (HILIC) is a significant enrichment strategy in glycoproteomics profiling. In this report, hydrophilic magnetic mesoporous silica materials (denoted as Fe3O4@mSiO2-IDA) were designed and synthesized an outstanding platform for glycopeptide analysis. By taking advantage of their merits, such large surface area, excellent hydrophilicity unbiased affinity toward all types glycopeptides, the Fe3O4@mSiO2-IDA nanomaterials successfully applied to...

The synthetic procedure of hydrophilic metal–organic frameworks by modifying glutathione on Au-immobilized magnetic MOFs.

Abstract For a deep understanding of the human proteome, first crucial step is always effective separation targeted proteins/peptides from complex samples. In mass spectrometry‐based proteomics, nanomaterials have emerged as highly means to separate and increase their relative abundance, which beneficial conduct spectrometric analysis. particular, with different specific functionalities received great attention in ordinary phosphoproteomics, glycoproteomics, for easily recognizable...

In this work, a magnetic covalent organic framework (COF) with inherent hydrophilicity (denoted mCTpBD) was synthesized through interface deposition of hydrophilic COF shell on amino group-functionalized magnetite particles via the reaction between carboxyl group-containing monomer and benzidine. Thanks to superior hydrophilicity, appropriate porous structure, easy separation, resulting mCTpBD exhibited excellent performance in conveniently enriching glycopeptides from standard samples high...

Gastric cancer (GC) presents high mortality worldwide because of delayed diagnosis. Currently, exosome-based liquid biopsy has been applied in diagnosis and monitoring diseases including cancers, whereas disease detection based on exosomes at the metabolic level is rarely reported. Herein, specific aptamer-coupled Au-decorated polymorphic carbon (CoMPC@Au-Apt) constructed for capture urinary from early GC patients healthy controls (HCs) subsequent exosome pattern profiling without extra...

Abstract Developing a standardized screening tool for the detection of early and small hepatocellular carcinoma (HCC) through urinary metabolic analysis poses challenging yet intriguing research endeavor. In this study, range intricately interlaced 2D rough nanosheets featuring well‐defined sharp edges is fabricated, with aim constructing diverse trimetal oxide heterojunctions exhibiting multiscale structures. By carefully engineering synergistic effects in composition structure, including...

Exosome metabolite-based liquid biopsy is a promising strategy for large-scale application in practical clinics toward precise medicine. Given the current challenges successive isolation and analysis of exosomes their metabolites this field, we established low-cost, high-throughput, rapid platform serological exosome metabolic hepatocellular carcinoma (HCC) via designed core-shell nanoparticles. It starts with efficient extraction high-quality serum features, based on which significantly...

Abstract The development of matrices has shown great potential for fluid metabolic analysis in disease detection. However, single‐fluid metabolomic been recognized as insufficient to fully capture the complexities diseases such liver disease, which limits detection accuracy. To this end, hollow dodecahedral nanocages‐based analytical tool is developed, featuring four‐high characteristics speed, throughput, efficiency, and patient compliance, enhance extraction multifluid profiles....

Developing cost-effective approaches based on hydrophilic interaction liquid chromatography (HILIC) has been the main tendency for low-abundance glycopeptides capture before LC-MS/MS analysis. Carbohydrates with outstanding biocompatibility and hydrophilicity are ubiquitous in kingdoms of animal plant could be a wonderful choice as functional groups enrichment. In this work, glucose-6-phosphate, one indispensable cogs pivotal metabolic wheels life, was chosen functionalized to grafted onto...

In this work, magnetic nanoporous carbon (NPC) materials were synthesized by choosing a MOF as sacrificial template and precursor. The obtained Co-ZIF-67 showed strong response, high surface area, uniform size of mesopores content. successfully applied to glycomics analysis enriching N-linked glycans in bio-samples with selectivity efficiency.

Developing an effective separation method is necessary for identifying low-abundant endogenous phosphorylated peptides with the removal of proteins. In this work, we prepared size-exclusive magnetic graphene/mesoporous silica composites titanium(IV)-immobilized pore walls (denoted as Ti4+-MGMSs) capturing mass spectrometry analysis. The introduction hydrophilic polydopamine simplified synthetic process Ti4+-MGMSs, and ordered mesoporous channels are beneficial to trapping phosphopeptides...

Highly efficient detection of endogenous phosphopeptides from complex biosamples is essential in phosphopeptidomics analysis due to the severe disturbance caused by chaotic biological environment. In this study, for highly selective capture phosphopeptides, a magnetic binary metal–organic framework (MOF) with Zr–O and Ti–O centers (denoted as Fe3O4@PDA@Zr-Ti-MOF) was designed synthesized facile postsynthetic method. Briefly, Zr-based MOF first coated on surface Fe3O4 polydopamine (PDA)...

In this article, the syntheses and applications of hydrophilic nanomaterials in glycoproteomics are reviewed.

Fe₃O₄@PDA@UIO-66-NH₂ is a biocompatible material that can be applied to enrich exosomes and phosphopeptides from urinary consecutively.

Abstract Currently, most Crohn's disease (CD) patients suffer from serious complications and even surgery due to delayed diagnosis; therefore, point‐of‐care diagnosis of CD is urgently required. In this work, a covalent organic framework@Au (COF‐V@Au) matrix material with excellent laser absorption prepared, endowing metabolites great ionization efficiency in desorption/ionization mass spectrometry (LDI‐MS) analysis. addition, the COF‐V@Au possesses 1093 m 2 g −1 large specific surface area...

Exosome metabolite-based noninvasive liquid biopsy is an emerging research hotspot that tends to substitute current means in clinics. Nanostructure-based mass spectrometry enables continuous exosome isolation and metabolic profiling with superior analysis speed high efficiency. Herein, we construct a heterogeneous MXene hybrid possesses ternary binding sites for capture outstanding matrix performance metabolite analysis. Upon optimizing experimental conditions, the average extraction of...

Exosomes, a growing focus for liquid biopsies, contain diverse molecular cargos. In particular, exosome metabolites with valuable information have exhibited great potential improving the efficiency of biopsies addressing complex medical conditions. this work, we design directional growth Ti-metal–organic frameworks on polar-functionalized magnetic particles. This facilitates rapid synergistic capture exosomes assistance an external field and additionally synergistically enhances ionization...

Multidimensional metabolic analysis has become a new trend in establishing efficient disease monitoring systems, as the constraints associated with relying solely on single dimension refined are increasingly pronounced. Here, coordination polymers employed derivative precursors to create multishell hollow hybrids, developing an integrated system. Briefly, fingerprints extracted from hundreds of serum samples and urine samples, encompassing not only membranous nephropathy but also related...

Abnormal protein glycosylation has been demonstrated to be associated with many diseases; therefore, it is very important conduct a comprehensive structure analysis of glycan for prognosis and diagnosis diseases, such as cancer. In this work, the first time, carbon-functionalized ordered graphene/mesoporous silica composites (denoted C-graphene@mSiO2) large surface area uniform pore size were designed synthesized. By taking advantage special interaction between carbon glycans well...