A5088048220- Extracellular vesicles in disease
- Metabolomics and Mass Spectrometry Studies
- Immune Cell Function and Interaction
- Mycorrhizal Fungi and Plant Interactions
- Mass Spectrometry Techniques and Applications
- Plant Pathogens and Fungal Diseases
- Advanced biosensing and bioanalysis techniques
- Insect Resistance and Genetics
- Glycosylation and Glycoproteins Research
- Plant biochemistry and biosynthesis
- Analytical Chemistry and Chromatography
- Immunotherapy and Immune Responses
- Advanced Proteomics Techniques and Applications
- MicroRNA in disease regulation
- Plant and Fungal Interactions Research
- Nanoplatforms for cancer theranostics
- Probiotics and Fermented Foods
- Single-cell and spatial transcriptomics
- Embedded Systems and FPGA Design
- Forensic Toxicology and Drug Analysis
- Gut microbiota and health
- Yeasts and Rust Fungi Studies
- Student Assessment and Feedback
- RNA and protein synthesis mechanisms
- Connexins and lens biology
Fudan University
2016-2024
Fujian Agriculture and Forestry University
2023-2024
Ministry of Education
2024
Zhongshan Hospital
2021-2022
Wuhan University
2021-2022
Chongqing Normal University
2022
The First Affiliated Hospital, Sun Yat-sen University
2021
Sun Yat-sen University
2021
Chinese Academy of Agricultural Sciences
2013-2021
Northeast Normal University
2021
Exosomes are endosome-derived small membrane vesicles that secreted by most cell types including tumor cells. Tumor-derived exosomes usually contain antigens and have been used as a source of to stimulate anti-tumor immune responses. However, many reports also suggest tumor-derived can facilitate evasion through different mechanisms, which antigen-independent. In the present study we mouse model delayed-type hypersensitivity (DTH) demonstrated local administration carrying antigen chicken...
Acute coronary syndrome (ACS), comprising unstable angina (UA) and acute myocardial infarction (AMI), is the leading cause of death worldwide. Currently, lacking effective strategies for classifying ACS hinders prognosis improvement patients. Disclosing nature metabolic disorders holds potential to reflect disease progress high-throughput mass spectrometry-based analysis a promising tool large-scale screening. Herein, hollow crystallization COF capsuled MOF hybrids (UiO-66@HCOF) assisted...
Precise early diagnosis and staging are conducive to improving the prognosis of colorectal cancer (CRC) gastric (GC) patients. However, due intrusive inspections limited sensitivity, prevailing diagnostic methods impede precisely large-scale screening. In this work, we reported a high-throughput serum metabolic patterns (SMP) screening strategy based on covalent organic frameworks-assisted laser desorption/ionization mass spectrometry (hf-COFsLDI-MS) for CRC GC. Notably, 473 high-quality SMP...
Rheumatoid arthritis (RA) is a chronic autoimmune disease and one of the leading causes disability in USA. Although certain biological therapies, including protein antibodies targeting inflammatory factors such as tumor necrosis factor, are effective reducing symptoms RA, these treatments do not reverse disease. Also, although novel gene therapy approaches have shown promise preclinical clinical studies to treat it still unclear whether can be readily safely applied large number RA patients....
Mycoplasmas cause numerous human diseases and are common opportunistic pathogens in cancer patients immunocompromised individuals. Mycoplasma infection elicits various host immune responses. Here we demonstrate that mycoplasma-infected tumor cells release exosomes (myco+ exosomes) specifically activate splenic B induce splenocytes cytokine production. Induction of cytokines, including the proinflammatory IFN-γ anti-inflammatory IL-10, was largely dependent on presence cells. were major IL-10...
Abstract Currently, most Crohn's disease (CD) patients suffer from serious complications and even surgery due to delayed diagnosis; therefore, point‐of‐care diagnosis of CD is urgently required. In this work, a covalent organic framework@Au (COF‐V@Au) matrix material with excellent laser absorption prepared, endowing metabolites great ionization efficiency in desorption/ionization mass spectrometry (LDI‐MS) analysis. addition, the COF‐V@Au possesses 1093 m 2 g −1 large specific surface area...
Genome mining of Aspergillus ustus 094102 enabled the discovery a multiproduct bifunctional terpene synthase (BTS), AuAS. Heterologous expression AuAS led to five new sesterterpenes, and coexpression upstream CYP450 monooxygenase (AuAP450) generated four sesterterpene alcohols. Additionally, aspergilol A showed cytotoxic activities against MCF-7, MDA-MB231, HepG2 cancer cells (IC50 21.20–48.76 μM), B exhibited effect on MCF-7 27.41 μM).
Leaves of Camellia sinensis plants are used to produce tea, one the most consumed beverages worldwide, containing a wide variety bioactive compounds that help promote human health. Tea cultivation is economically important, and its sustainable production can have significant consequences in providing agricultural opportunities lowering extreme poverty. Soil parameters well known affect quality resultant leaves consequently, understanding diversity functions soil microorganisms tea gardens...
Although triple-negative breast cancer (TNBC) is the most refractory subtype among all cancers, it has been shown to have higher immune infiltration than other subtypes. We identified marine-derived small molecule MHO7, which acts as a potent immunogenic cell death (ICD) inducer through endoplasmic reticulum (ER) stress-C/EBP-homologous protein (CHOP) pathway, treat TNBC. MHO7 exerted cytostatic and cytotoxic effects on TNBC cells at an IC50 of 0.96-1.75 µM suppressed tumor growth with...
Diabetes and high myopia as well-known high-risk factors can aggravate cataracts, yet clinical coping strategy remains a bottleneck. Metabolic analysis tends to be powerful for precisely detection mechanism exploration since most of diseases including cataracts are accompanied by metabolic disorder. Herein, layered binary co-ionizers assisted aqueous humor tool is proposed potentially etiological typing age-related (ARC), with diabetes mellitus (CDM), (CHM). Startlingly, taking advantage the...
The characterization of natural fungal diversity impacts our understanding ecological and evolutionary processes can lead to novel bioproduct discovery. Russula Lactarius, both in the order Russulales, represent two large genera ectomycorrhizal fungi that include edible as well toxic varieties. Based on morphological phylogenetic analyses, including nucleotide sequences internal transcribed spacer (ITS), 28S subunit ribosomal RNA (LSU), second largest polymerase II (RPB2), mitochondrial...
Tumor-specific immunosuppression is frequently observed in tumor-bearing hosts. Exosomes are nano-sized, endosomal-derived membrane vesicles secreted by most tumor and hematopoietic cells have been shown to actively participate immune regulation. We previously demonstrated that antigen-specific immunosuppressive exosomes could be isolated from the blood plasma of antigen-immunized mice. Here, we demonstrate plasma-derived mice bearing OVA-expressing tumors were able suppress OVA-specific...
Nonalcoholic fatty liver disease (NAFLD), along with its progressive forms nonalcoholic steatohepatitis (NASH) and NASH fibrosis, has emerged as a global health crisis. However, the absence of robust screening risk evaluation tools contributes to underdiagnosis NAFLD. Herein, we reported multichannel nanogenerator-assisted laser desorption/ionization mass spectrometry (LDI-MS) platform for early Specifically, titanium oxide nanosheets (TiNS) covalent-organic framework (COFNS) were employed...
The IL‐12 family of heterodimeric cytokines, consisting IL‐12, IL‐23, IL‐27, and IL‐35, has important roles in regulating the immune response. members are comprised a heterodimer α β chains: (p40 p35), IL‐23 p19), IL‐27 (Ebi3 p28), IL‐35 p35). Given combinatorial nature family, we generated adenoviral vectors expressing two putative not yet found naturally, termed IL‐X p19) IL‐Y as single‐chain molecules. Single chain (scIL‐Y), but scIL‐X, was able to stimulate significantly unique...
Exosomes are regarded as the emerging potential targets for liquid biopsy and bioprocess study owing to their abundant inclusive cargos that carry significant disease information. In addition, metabolites have been promising biomarkers diagnosis. However, little metabolic research on exosomes is carried out by now. Herein, mix‐crystal titania‐assisted laser desorption/ionization mass spectrometry (LDI‐MS) method established, which features fast speed, high throughput, efficiency, directly...
This study evaluated the cholesterol-lowering property of lactic acid bacteria (LAB) isolated from mink. Two strains, Enterococcus faecium MDF1104 and Lactobacillus plantarum MDL1118, were shown to remove cholesterol broths natural hen egg yolk skimmed milk. The in was reduced by 58.15% 38% L. E. faecium, respectively. When used combination, 48.95% (p < 0.01) removed Experimental mice remained healthy when fed different doses LAB, total serum concentration lowest (0.90 mmol/L) a combination...
Serum N-glycan patterns from 50 Crohn's disease (CD) patients and healthy controls were acquired using a carbon matrix, which eight N-glycans with significant difference screened out to reveal remarkale performance for CD diagnosis. This research is expected help future glycan-based detection not limited CD.
Ultra-low-copy number proteins play a crucial role in exploring cellular heterogeneity and the insight of protein biomarkers single cell. However, counting ultra-low-copy target cell remains grand challenge. Herein, we developed so-called single-cell picoliter liquid operating technology for An ingenious volume-controllable sampling technique was employed to capture subsequent analysis. Remarkably, 50 pL sample volume preparation, capture, in-droplet lysis, immobilization on functionalized...
Introduction Understanding microbial communities in diverse ecosystems is crucial for unraveling the intricate relationships among microorganisms, their environment, and ecosystem processes. In this study, we investigated differences fungal community structure diversity soils from two contrasting climatic vegetation conditions: Xinjiang western China plateau Fujian southeastern coastal province. Methods A total of 36 soil samples collected regions were subjected to high-throughput ITS gene...
A new species of Amanita, A. minqingensis, is described from Fujian province, China, based on morphological and multi-phylogenetic sequencing including the nuclear ribosomal large subunit (nrLSU), internal transcribed spacer (ITS), RNA polymerase subunit-2 (rpb2), translation elongation factor-α (tef1-α) loci. We identify as a member in Amanita section Vaginatae, subg. Amanita. The characterized by its tan pileus with putty verrucose volval remnants, absence clamps globose to sub-globose...