A5078007274- Asthma and respiratory diseases
- Drug-Induced Adverse Reactions
- MicroRNA in disease regulation
- Urticaria and Related Conditions
- Eosinophilic Esophagitis
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Allergic Rhinitis and Sensitization
- Extracellular vesicles in disease
- RNA Research and Splicing
- Eosinophilic Disorders and Syndromes
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Contact Dermatitis and Allergies
- Protease and Inhibitor Mechanisms
- Respiratory and Cough-Related Research
- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- Pharmacovigilance and Adverse Drug Reactions
- IL-33, ST2, and ILC Pathways
- Enzyme Structure and Function
- Inhalation and Respiratory Drug Delivery
- Circular RNAs in diseases
- Bacteriophages and microbial interactions
- Food Allergy and Anaphylaxis Research
- Immune Response and Inflammation
Mount Nittany Medical Center
2018-2024
Pennsylvania State University
2013-2024
Penn State Milton S. Hershey Medical Center
2012-2024
Hershey (United States)
2016-2019
National Institute on Aging
2016
National Institutes of Health
2016
Washington State University Spokane
2016
Allergy, Asthma and Clinical Research Center
2010
Johns Hopkins University
2007-2008
Johns Hopkins Medicine
2007
Abstract HuR is a regulator of mRNA turnover or translation inflammatory genes through binding to adenylate-uridylate–rich elements and related motifs present in the 3′untranslated region (UTR) mRNAs. We postulate that critically regulates epithelial response by associating with multiple ARE-bearing, functionally transcripts. aimed identify targets human airway cell line BEAS-2B challenged TNF-α plus IFN-γ, strong stimulus for responses. Ribonucleoprotein complexes from resting...
Abstract Exposure to cockroach allergen is a strong risk factor for developing asthma. Asthma has been associated with allergen-induced airway epithelial damage and heightened oxidant stress. In this study, we investigated allergen–induced oxidative stress in epithelium its underlying mechanisms. We found that extract (CRE) could induce reactive oxygen species (ROS) production, particularly mitochondrial-derived ROS, human bronchial cells. then used the RT2 Profiler PCR array identified...
Glucocorticoids (GCs) are the mainstay of anti-inflammatory therapy. Modulation posttranscriptional regulation (PTR) gene expression by GCs is a relevant yet poorly characterized mechanism their action. The RNA-binding protein tristetraprolin (TTP) plays central role in PTR binding to AU-rich elements 3'-untranslated region proinflammatory transcripts and accelerating decay. We found that induce TTP primary immortalized human bronchial epithelial cells. To investigate importance GC function,...
Abstract Posttranscriptional regulation is emerging as a key factor in glucocorticoid (GC)-mediated gene regulation. We investigated the role of human GC receptor (GR) an RNA-binding protein and its effect on mRNA turnover airway epithelial cells. Cell treatment with potent budesonide accelerated decay CCL2 (t1/2 = 8 ± 1 min versus 62 17 DMSO-treated cells) CCL7 15 4 114 37 min), but not that CCL5 (t1/2=231 266 5 min) BEAS-2B cell line. This was inhibited by preincubation anti-GR Ab,...
Background MicroRNAs (miRNAs) are emerging as central regulators of inflammation, but their role in asthma and airway epithelial cells is not well studied. Glucocorticoids the cornerstone therapy other inflammatory disease, yet mechanisms action completely elucidated, it clear whether miRNAs modulate effects. Objective We aimed to identify that regulate cytokine chemokine expression these subject effects glucocorticoids. Methods results MicroRNAomic analyses immortalized, normal human...
The UDP-glucuronosyltransferase (UGT) 2B enzymes are important in the detoxification of a variety endogenous and exogenous compounds, including many hormones, drugs, carcinogens. Identifying novel mechanisms governing their expression is understanding patient-specific response to drugs cancer risk factors. In silico prediction algorithm programs were used screen for microRNAs (miRNAs) as potential regulators UGT2B enzymes, with miR-216b-5p identified candidate. Luciferase data suggested...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTMapping Protein−Protein Interactions in the Bacteriophage T4 DNA Polymerase Holoenzyme Using a Novel Trifunctional Photo-cross-linking and Affinity ReagentStephen C. Alley, Faoud T. Ishmael, A. Daniel Jones, Stephen J. BenkovicView Author Information Department of Chemistry Biochemistry Molecular Biology Hershey Medical Center The Pennsylvania State University, 415 Wartik Laboratory University Park, 16802 Cite this: Am. Chem. Soc. 2000, 122,...
miR-511-3p, encoded by CD206/Mrc1, was demonstrated to reduce allergic inflammation and promote alternative (M2) macrophage polarization. Here, we sought elucidate the fundamental mechanism which miR-511-3p attenuates promotes Compared with WT mice, allergen-challenged Mrc1-/- mice showed increased airway hyperresponsiveness (AHR) inflammation. However, this AHR were significantly attenuated when these pretransduced adeno-associated virus-miR-511-3p (AAV-miR-511-3p). Gene expression...
Beta-lactam antibiotics are widely used, but hypersensitivity reactions common and difficult to manage. This study was designed identify lack of knowledge regarding the safe use alternative beta-lactams in penicillin-allergic patients assess management differences between allergists nonallergists. An electronic physician survey sent 623 providers allergy, internal medicine, pediatrics, family querying beta-lactam with a history penicillin allergy. A total 110 (17.7%) surveys were completed....
The bacteriophage T4 59 protein (gp59) plays a vital role in recombination and replication by promoting the assembly of gene 41 helicase (gp41) onto DNA, thus enabling as well strand exchange recombination. Loading gp32 (the single binding protein)-coated single-stranded DNA requires gp59 to remove replace it with gp41. Cross-linking studies between reveal an interaction Cys-166 Cys-42 gp59. Since lies core domain gp32, this may affect association DNA. In presence or is capable forming...
The bacteriophage T4 replication complex is composed of eight proteins that function together to replicate DNA. This replisome can be broken down into four basic units: a primosome gp41, gp61, and gp59; leading strand holoenzyme gp43, gp44/62, gp45; lagging holoenzyme; single binding protein polymer. These units interact further form the complete replisome. polymerases are physically linked in presence DNA or an active region interaction was mapped extension finger domain, such Cys-507 one...
Within replisomes for DNA replication, the primosome is responsible unwinding double-stranded and synthesizing RNA primers. Assembly of bacteriophage T4 on individual molecules ssDNA or forked (fDNA) has been studied by using FRET microscopy. On either substrate, an ordered process assembly begins with tight 1:1 binding ssDNA-binding protein (gp32) helicase-loading (gp59) to DNA. Magnesium adenosine 5′- O -(3-thiotriphosphate) (MgATPγS) mediates weak helicase (gp41) coated gp32 gp59, whereas...
Abstract Background The respiratory epithelium plays a central role in the inflammatory response asthma and other diseases. Methoxyphenolic compounds are purported to be effective anti-inflammatory agents, but their effects on airway have not been well characterized. Methods Human cells were stimulated with TNF-α presence or absence of 4-substituted methoxyphenols resveratrol. expression various cytokines was measured by qPCR, ELISAs, protein arrays. Reactive oxygen species (ROS) production...
The gene product 61 primase protein from bacteriophage T4 was expressed as an intein fusion and purified to homogeneity. binds one zinc ion, which is coordinated by four cysteine residues form a ribbon motif. Factors that influence the rate of priming were investigated, physiologically relevant ∼1 primer per second primosome achieved. Primase binding single-stranded (1 primase:4 gp32 monomers; Kd ∼ 860 nM) helicase in presence DNA ATP-γ-S primase:1 monomer; 100 investigated isothermal...
The bacteriophage T4 59 protein (gp59) plays an essential role in recombination and replication by mediating the assembly of gene 41 helicase (gp41) onto DNA. gp59 is required to displace gp32 single-stranded binding on lagging strand expose a site for binding. To gain better understanding mechanism assembly, architecture stoichiometry gp41-gp59 complex were investigated. Both N C termini gp41 found lie close or gp41-gp41 subunit interface interact with gp59. interaction proximal Cys-215...