A5002648257- Bacterial Genetics and Biotechnology
- Urinary Tract Infections Management
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Pediatric Urology and Nephrology Studies
- Bacterial biofilms and quorum sensing
- Protein Structure and Dynamics
- Microbial Community Ecology and Physiology
- Spaceflight effects on biology
- ATP Synthase and ATPases Research
- Urinary Bladder and Prostate Research
- Vibrio bacteria research studies
- Enzyme Structure and Function
- Adenosine and Purinergic Signaling
- Bacterial Identification and Susceptibility Testing
- Colorectal Cancer Screening and Detection
- Escherichia coli research studies
- Biochemical and Molecular Research
- vaccines and immunoinformatics approaches
- Probiotics and Fermented Foods
- Light effects on plants
- Biochemical and Structural Characterization
- Bacillus and Francisella bacterial research
University of Alabama in Huntsville
2019-2025
Duke University
2016-2019
Loyola University Chicago
2018
Pennsylvania State University
2008-2014
Harvard University
2013-2014
Park University
2014
Protein secretion typically involves translocation of unfolded polypeptides or transport monomeric folded proteins. Here we provide, to our knowledge, the first experimental evidence for an intact multimeric complex requiring a signal formed by both members complex. Using systematic mutagenesis substrate involved in early secretory antigen 6 kDa (ESX) Bacillus subtilis, demonstrate that export requires two independent motifs. mixed dimers, show these motifs must form composite which one...
Pseudomonas aeruginosa is an opportunistic pathogen that often infects open wounds or patients with cystic fibrosis. Once established, P. infections are notoriously difficult to eradicate. This difficulty in part due the ability of tolerate antibiotic treatment at individual-cell level through collective behaviors. Here, we describe a new phenomenon by which tolerates treatment. In particular, sublethal concentrations antibiotics covering all major classes promoted accumulation...
Here, we report the draft genomes of Klebsiella pneumoniae 5008-1 and Streptococcus anginosus 5008-2 strains isolated from a catheterized urine sample obtained an asymptomatic postmenopausal woman diagnosed with recurrent urinary tract infection receiving vaginal estrogen cream.
Overactive bladder syndrome (OAB) is a poorly understood symptom complex that affects 40% of females over the age 40, with clinical features including urinary urgency and incontinence. In addition to inflammation, oxidative stress, nerve damage reduced blood flow, alterations in microbiome (urobiome), specifically bacterial diversity, have been reported be associated OAB. Bladder bacteria are members urobiome along viruses, archaea, fungi, protozoans. The metabolism, particularly...
Abstract The urinary microbiome has been increasingly characterized using next-generation sequencing. However, many of the technical methods have not yet specifically optimized for urine. We sought to compare performance several DNA isolation kits used in studies. A total 11 voided urine samples and one buffer control were divided into 5 equal aliquots processed parallel five commercial kits. was quantified V4 segment 16S rRNA gene sequenced. Data identify microbial composition assess alpha...
The human urinary microbiome is thought to affect the development and progression of tract infections (UTI), particularly recurrent UTIs in aging populations women. To understand possible interactions pathogens with commensal bacteria inhabiting bladder, we conducted an initial functional assessment a representative set lactobacilli that dominate this niche postmenopausal We created repository bladder isolated
It is largely unknown how the typical homomeric ring geometry of ATPases associated with various cellular activities enables them to perform mechanical work. Small-angle solution X-ray scattering, crystallography, and electron microscopy (EM) reconstructions revealed that partial ATP occupancy caused heptameric closed bacterial enhancer-binding protein (bEBP) NtrC1 rearrange into a hexameric split striking asymmetry. The highly conserved functionally crucial GAFTGA loops responsible for...
Biofilms are self-organized communities of microorganisms that encased in an extracellular polymeric matrix and often found attached to surfaces. widely present on Earth, diverse sometimes extreme environments. These microbial have been described as recalcitrant or protective when facing adversity environmental exposures. On the International Space Station, biofilms were human-inhabited environments a multitude hardware Moreover, studies identified phenotypic genetic changes under...
Abstract Plasmid conjugation is a driving force behind the spread of antibiotic resistance genes. While problematic for medical purposes, can be used delivery target genes in microbial communities, example, bioremediation. Physiological state host bacterial cell strongly affects transfer prototypic F‐plasmid Escherichia coli , but it remains unclear whether such modulation wide‐spread. We tested broad range Enterobacteriaceae conjugative plasmids their sensitivity to growth stage donors and...
Hospital-acquired infections are considered a priority for public health systems, which poses significant burden society. High-touch surfaces of healthcare centers, including textiles, provide suitable environment pathogenic bacteria to grow, necessitating incorporating effective antibacterial agents into textiles. This paper introduces highly durable gel-like solution, Silver Shell finish, contains chitosan-bound silver chloride microparticles. The study investigates the coating's...
Abstract This study reports an experimental comparison of two types atmospheric pressure plasma jets in terms their fundamental characteristics and efficacy bacterial sterilization. The are fabricated by inserting a high voltage electrode inside one-end closed (double DBD jet) or both ends open (single quartz tubes which further enclosed second tube containing ground electrode. Both operated contact with water surface using unipolar pulsed DC power supply helium as the working gas. Results...
Bacterial EBPs (enhancer-binding proteins) play crucial roles in regulating cellular responses to environmental changes, part by providing efficient control over σ54-dependent gene transcription. The AAA+ (ATPase associated with various activites) domain of the EBPs, when assembled into a ring, uses energy from ATP binding, hydrolysis and product release remodel σ54–RNAP (RNA polymerase) holoenzyme so that it can transition closed open form at promoter DNA. assembly, hence activity, these...
Many bacteria take up DNA from their environment as part of the process natural transformation. uptake allows microorganisms to gain genetic diversity and can lead spread antibiotic resistance or virulence genes within a microbial population. Development competence (Com) in Bacillus subtilis is highly regulated that culminates expression several late formation apparatus. The operon comF encodes small protein unknown function, ComFB. To insight into function ComFB, we determined its 3D...
The human urinary bladder hosts a complex microbial community of low biomass referred to as the urobiome. While composition urobiome has been investigated in adults for over decade now, only few studies have considered presence and children. It is critical explore how develops throughout life span it changes various health conditions. Therefore, we set review available data on pediatric its development with age disease. In addition, focused identifying reporting specific gaps our knowledge...
We report draft genomes of five bacteria recovered from the U.S. and Russian water systems onboard International Space Station. The genera include Ralstonia, Burkholderia, Cupriavidus, Methylobacterium, Pseudomonas. These sequences will help further understanding reclamation environmental control life support in space.
Except for apyrases, ATPases generally target only the gamma-phosphate of a nucleotide. Some non-apyrase from thermophilic microorganisms are reported to hydrolyze ADP as well ATP, which has been described novel property extreme thermophiles. Here, we describe an apparent hydrolysis by highly purified preparations AAA+ ATPase NtrC1 extremely bacterium, Aquifex aeolicus. This activity is actually combination activities and contaminating adenylate kinase (AK) Escherichia coli, present at...
Here, we present the draft genome sequences of