A5060042938- Sarcoma Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- CAR-T cell therapy research
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- Chromosomal and Genetic Variations
- Cancer Immunotherapy and Biomarkers
- Protein Degradation and Inhibitors
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Genetic diversity and population structure
- Urinary Tract Infections Management
- Plant and animal studies
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Genomics and Phylogenetic Studies
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Effects of Radiation Exposure
- Gastrointestinal Tumor Research and Treatment
- Plant Gene Expression Analysis
- Histone Deacetylase Inhibitors Research
- Plant Pathogens and Resistance
Duke University
2008-2025
University of North Carolina at Chapel Hill
2015-2024
UNC Lineberger Comprehensive Cancer Center
2024
Duke Medical Center
2021-2023
Duke University Hospital
2023
University of North Carolina Health Care
2016
North Carolina State University
2002-2006
Abstract Variation in inflorescence development patterns is a central factor the evolutionary ecology of plants. The genetic architectures 13 traits associated with developmental timing, architecture, rosette morphology, and fitness were investigated Arabidopsis thaliana, model plant system. There substantial naturally occurring variation for traits, broad sense heritabilities computed from 21 ecotypes ranging 0.134 to 0.772. Genetic correlations are significant most (64/78) pairs suggesting...
For most eukaryotes, sexual reproduction is a fundamental process that requires meiosis. In turn, meiosis typically depends on reciprocal exchange of DNA between each pair homologous chromosomes, known as crossover (CO), to ensure proper chromosome segregation. The frequency and distribution COs are regulated by intrinsic extrinsic environmental factors, but much more about the molecular mechanisms governing former compared latter. Here we show elevated temperature induces meiotic...
Cannabis use alters sperm DNA methylation, but the potential reversibility of these changes is unknown. Semen samples from cannabis users and non-user controls were collected at baseline again following a 77-day period abstinence (one spermatogenic cycle). Users did not significantly differ by demographics or semen analyses. Whole-genome bisulfite sequencing identified 163 CpG sites with different methylation in between groups (
Abstract Background Cannabis legalization is expanding and men are the predominant users. We have limited knowledge about how cannabis impacts sperm whether effects heritable. Results Whole genome bisulfite sequencing (WGBS) data were generated for of rats exposed to: (1) extract (CE) 28 days, then 56 days vehicle only (~ one spermatogenic cycle); (2) CE; or (3) only. Males mated with drug-naïve females to produce F1 offspring from which heart, brain, tissues underwent analyses. There 3321...
A forward genetic screen is one of the best methods for revealing function genes. In plants, this technique highly efficient, as it relatively easy to grow and hundreds or thousands individuals. The cost efficiency ease data production afforded by next-generation sequencing have created new opportunities rapid mapping induced mutations. Current tools are often not user friendly, complicated, require extensive preparation steps. To simplify process mutations, we developed a pipeline that...
Here, we report the draft genomes of Klebsiella pneumoniae 5008-1 and Streptococcus anginosus 5008-2 strains isolated from a catheterized urine sample obtained an asymptomatic postmenopausal woman diagnosed with recurrent urinary tract infection receiving vaginal estrogen cream.
Abstract Polyploidy (whole‐genome duplication) has contributed significantly to angiosperm evolution and diversification. To date, it been found that most polyploids are the result of multiple formation events, which may contribute genetic diversity affect interfertility among polyploid lineages independent origin. A recently discovered allotetraploid derivative Mimulus guttatus M. nasutus , sookensis is throughout valleys western Oregon Vancouver Island. Here, we analyse patterns nucleotide...
While recent breakthroughs have enabled intense study of bacterial DNA modifications, limitations in current work potentiated a surprisingly untested narrative that methylation is common mechanism the response to environmental conditions. Essentially, whether epigenetic regulation transcription common, generalizable phenomenon critical unanswered question we address here.
Background: APOE4 has been hypothesized to increase Alzheimer’s disease risk by increasing neuroinflammation, though the specific neuroinflammatory pathways involved are unclear. Objective: Characterize cerebrospinal fluid (CSF) proteomic changes related copy number. Methods: We analyzed targeted data from ADNI CSF samples using a linear regression model adjusting for age, sex, and number, additional models also AD clinical status or Aβ, tau, p-tau levels. False discovery rate was used...
Transcription factors (TFs) regulate the differentiation of T cells into diverse states with distinct functionalities. To precisely program desired cell in viral infections and cancers, we generated a comprehensive transcriptional epigenetic atlas nine CD8 + for TF activity prediction. Our analysis catalogued fingerprints each state, uncovering new regulatory mechanisms that govern selective state differentiation. Leveraging this platform, focused on two critical tumor virus control:...
ABSTRACT Post-traumatic stress disorder is a mental that may occur in the aftermath of severe psychological trauma. We examined 1,065,750 DNA methylation (DNAm) sites from 171 donors including neurotypicals, PTSD, and major depressive cases across six areas implicated fear circuitry brain. found significant differential for PTSD near 195 genes utilizing cross-region modeling, identified 6,641 candidate genes. Approximately 26% differentially methylated CpGs were present risk loci PTSD. To...
A new species of monkeyflower, Mimulus sookensis, is described. This found throughout the southern portion Vancouver Island, Gulf Islands British Columbia, San Juan Washington state, Willamette and Umpqua River Valleys in Oregon, has been collected at one location Mendocino County, California. sookensis a tetraploid (n = 28) derived from predominately outcrossing guttatus DC. 14) self-pollinating nasutus Greene 14). similar phenotypically to small-flowered M. nasutus, but differs chromosome...
730 Background: ICIs are effective in mRCC, but one pertinent clinical need is to identify predictive biomarkers for response. The PD-1 receptor has been implicated regulating gastrointestinal commensal bacteria, with varied immune interactions, thereby impacting response ICIs. We evaluated bacterial taxa and ICI outcomes mRCC pts. Methods: Fecal samples from 22 pts were collected at baseline, week (wk)-4 on ICI, upon disease progression. Pts grouped as responders (R, complete or partial...
Abstract Interferon regulatory factor 1 (IRF1) is a critical component of cell-intrinsic innate immunity that regulates both constitutive and induced antiviral defenses. Due to its short half-life, IRF1 function generally considered be regulated by synthesis. However, how activity controlled post-translationally has remained poorly characterized. Here, we employed proteomics approach identify proteins interacting with IRF1, found CSNK2B, subunit casein kinase 2, interacts directly...
Abstract This study aims to investigate whether adding neoadjuvant radiotherapy (RT), anti-programmed cell death protein-1 (PD-1) antibody (anti–PD-1), or RT + anti-PD-1 surgical resection improves disease-free survival for mice with soft tissue sarcomas (STS). We generated a high mutational load primary mouse model of STS by intramuscular injection adenovirus expressing Cas9 and guide RNA targeting Trp53 3-methylcholanthrene (MCA) into the gastrocnemius muscle wild-type (p53/MCA model)....
Knowledge regarding the origin and maintenance of hybrid zones is critical for understanding evolutionary outcomes natural hybridization. To evaluate contribution historical contact vs. long‐distance gene flow in formation a broad zone central northern Georgia that involves Aesculus pavia , A. sylvatica flava three cpDNA regions ( matK trnD‐trnT trnH‐trnK ) were analyzed. The maternal inheritance was confirmed via sequencing from progeny controlled crosses. Restriction site analyses...
Genetic variation is the fundamental medium of evolution. In allopolyploids, which are product hybridization and whole genome duplication, if homologous chromosomes always pair, then all descendants a single diploid F1 hybrid lineage will be genetically identical. Contrarily, genetic among initially isogenic lineages augmented when homeologous pair; this added may contribute to phenotypic Mimulus sookensis naturally occurring, small-flowered allotetraploid derived from large-flowered...