A5043314840- Mosquito-borne diseases and control
- Malaria Research and Control
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Identification and Quantification in Food
- Insect Pest Control Strategies
- Vibrio bacteria research studies
- Zoonotic diseases and public health
- Global Maternal and Child Health
- Parasitic Diseases Research and Treatment
- Iron Metabolism and Disorders
Michigan State University
2013-2025
Papua New Guinea Institute of Medical Research
2010-2025
Moscow Institute of Thermal Technology
2024
University of California, Irvine
2024
University of Malawi
2020
Malawi University of Business and Applied Sciences
2020
Michigan United
2013-2016
State University of New York
2015
The University of Queensland
2011
Behavioral resilience in mosquitoes poses a significant challenge to mosquito control. Although behavior changes anopheline vectors have been reported over the last decade, there are no empirical data suggest they compromise efficacy of vector control reducing malaria transmission.In this study, we quantified human exposure both bites and infective major Papua New Guinea course 4 years surrounding nationwide bednet distribution. We also infection prevalence population during same time...
Understanding mosquito host choice is important for assessing vector competence or identifying disease reservoirs. Unfortunately, the availability of an unbiased method comprehensively evaluating composition insect blood meals very limited, as most current molecular assays only test presence a few pre-selected species. These approaches also have limited ability to identify multiple mammalian hosts in single meal. Here, we describe novel high-throughput sequencing that enables analysis 96...
The major malaria vectors of Papua New Guinea exhibit heterogeneities in distribution, biting behaviour and infection levels. Long-lasting, insecticide-treated nets (LLINs), distributed as part the National Malaria Control Programme, are primary intervention targeting transmission. This study evaluated impact LLINs on anopheline density, species composition, feeding behaviour, Mosquitoes were collected by human landing catch 11 villages from East Sepik Province Madang Province. for 3 years...
Abstract Background Control of malaria parasite transmission can be enhanced by understanding which human demographic groups serve as the infectious reservoirs. Because vector biting heterogeneous, some infected individuals may contribute more to human-to-mosquito than others. Infection prevalence peaks in school-age children, but it is not known how often they are fed upon. Genotypic profiling blood permits identification individual humans who were bitten. The present investigation used...
Eastern equine encephalomyelitis virus (EEEV) is endemic in Michigan, showing an upsurge human cases and infections of white-tailed deer, horses, other animals the past decade (2010–2020). However, blood-host associations enzootic mosquito vector Culiseta melanura Great Lakes region are poorly known compared with better-studied regions. Vertebrate sources blood meals Cs. collected from resting boxes were determined through sequencing mitochondrial cytochrome B gene generated polymerase chain...
Host selection is an important determinant of vectorial capacity because malaria transmission increases when mosquitoes feed more on humans than non-humans. also affects the outcome long-lasting insecticidal nets (LLIN). Despite recent nationwide implementation LLIN-based control program in Papua New Guinea (PNG), little known about host local Anopheles vectors. This study investigated vectors PNG. Blood-engorged were sampled using barrier screen method and blood meals analyzed for...
The unprecedented global efforts for malaria elimination in the past decade have resulted altered vectorial systems, vector behaviors, and bionomics. These changes combined with increasingly evident heterogeneities transmission require innovative control strategies addition to established practices of long-lasting insecticidal nets indoor residual spraying. Integrated management will focal tailored achieve elimination. This switch emphasis from universal coverage plus additional...
The relationship between mosquito vectors and lymphatic filariasis (LF) parasites can result in a range of transmission outcomes. Anophelines are generally characterized as poor due to an inability support development at low densities. However, it is important understand the potential for natural maximize success elimination efforts. Primary Papua New Guinea (n = 1209) were dissected following exposure microfilaremic blood (range 8–233 mf/20 µl). We examined density dependent...
Abstract Malaria risk is highly heterogeneous. Understanding village and household-level spatial heterogeneity of malaria can support a transition to spatially targeted interventions for elimination. This analysis uses data from cross-sectional prevalence surveys conducted in 2014 2016 two villages (Megiar Mirap) Papua New Guinea. Generalised additive modelling was used characterise investigate the contribution individual, household environmental-level factors. Following period declining...
Access to human hosts by Anopheles mosquitoes is a key determinant of vectorial capacity for malaria, but it can be limited use long-lasting insecticidal nets (LLINs). In Malawi, pyrethroid-treated LLINs with and without the synergist piperonyl butoxide (PBO) were distributed control malaria. This study investigated blood-feeding patterns malaria vectors whether containing pyrethroid PBO led reduction blood feeding than those only pyrethroids.Mosquitoes sampled inside houses from May 2019...
Community composition of Anopheles mosquitoes, and their host-seeking peridomestic behaviour, are important factors affecting malaria transmission. In this study, barrier screen sampling was used to investigate species composition, abundance, nocturnal activity populations in villages Papua New Guinea.Mosquitoes were sampled from 6 pm am five 2012 2016. The screens positioned between the village houses perimeter where cultivated wild vegetation ("the bush") grew thickly. Female that rested...
A malaria control programme based on distribution of long-lasting insecticidal bed nets (LLINs) and artemisinin combination therapy began in Papua New Guinea 2009. After implementation the programme, substantial reductions vector abundance transmission intensity occurred. The research reported here investigated whether these remained after seven years sustained effort.All-night (18:00 to 06:00) mosquito collections were conducted using human landing catches barrier screen methods four...
Malaria and filariasis are transmitted in the Southwest Pacific region by Anopheles punctulatus sibling species including An. , koliensis farauti complex 1–8 (includes hinesorum [ 2], torresiensis 3]). Distinguishing these from each other requires molecular diagnostic methods. We developed a multiplex polymerase chain reaction (PCR)–based assay specific for known species-specific nucleotide differences internal transcribed spacer 2 identified five most frequently implicated transmitting...
Nonrandom selection and multiple blood feeding of human hosts by Anopheles mosquitoes may exacerbate malaria transmission. Both patterns their relationship to epidemiology were investigated in vectors Papua New Guinea (PNG). Blood samples from humans mosquito meals collected villages genetic profiles ("fingerprints") analyzed genotyping 23 microsatellites a sex-specific marker. Frequency acquired different humans, identified unique profiles, was fitted Poisson negative binomial distributions...
The development of insecticide resistance has compromised mosquito control efforts in many parts the world. Papua New Guinea (PNG) a long history dichlorodiphenyltrichloroethane (DDT) use and currently distributes pyrethroid-treated nets for malaria control. This study is first to investigate status pyrethroid Anopheles punctulatus group, major filariasis vectors PNG. used World Health Organization standard susceptibility bioassays detect knockdown phenotypes novel nested polymerase chain...
Anopheles punctulatus sibling species ( An. s.s., koliensis , and farauti complex [eight cryptic species]) are principal vectors of malaria filariasis in the Southwest Pacific. Given significant effort to reduce transmission through insecticide-treated net distribution region, effective strategies monitor evolution insecticide resistance among An . is essential. Mutations voltage-gated sodium channel VGSC ) gene have been associated with knock-down kdr pyrethroids DDT malarious regions. By...
Abstract Background Determination of blood-meal hosts in blood-fed female Anopheles mosquitoes is important for evaluating vectorial capacity vector populations and assessing effectiveness control measures. Sensitive molecular methods are needed to detect traces host blood mosquito samples, differentiate hosts, mixed meals. This paper describes a probe-based quantitative PCR identifying malaria vectors from Papua New Guinea. Methods TaqMan oligonucleotide probes targeting specific regions...
Implementation of long-lasting insecticide-treated net (LLIN) programs to control human malaria transmission leads substantial reductions in the abundance
Genetic profiling has been used to link mosquito bloodmeals the individual humans, but this analysis not done for other mammalian bloodmeals. In study, we describe a microsatellite-based method identifying pigs in based on their unique multilocus genotypes. Eleven tetranucleotide microsatellites and sex-specific marker were selected Smith-Waterman DNA sequence alignment scores from reference genome primers designed with features that reduce primer dimers, promote complete adenylation, enable...
Abstract Background: Determination of blood-meal hosts in blood-fed female Anopheles mosquitoes is important for evaluating vectorial capacity vector populations and assessing effectiveness control measures. Sensitive molecular methods are needed to detect traces host blood mosquito samples, differentiate hosts, mixed meals. This paper describes a probe-based quantitative PCR identifying malaria vectors from Papua New Guinea. Methods: TaqMan oligonucleotide probes targeting specific regions...