A5082821314- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Light effects on plants
- Marine and coastal ecosystems
- Bacteriophages and microbial interactions
- bioluminescence and chemiluminescence research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
Institut de Biosciences et Biotechnologies
2021-2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021-2023
Centre National de la Recherche Scientifique
2019-2023
CEA Cadarache
2021-2023
Institut de Biologie Intégrative de la Cellule
2021-2023
Université Paris-Saclay
2021-2023
CEA Paris-Saclay
2021-2023
Aix-Marseille Université
2019-2021
Institut de Microbiologie de la Méditerranée
2019
Light makes light work of fatty acids Photosynthetic organisms are notable for their ability to capture energy and use it power biosynthesis. Some algae have gone a step beyond photosynthesis can initiate enzymatic photodecarboxylation acids, producing long-chain hydrocarbons. To understand this transformation, Sorigué et al. brought bear an array structural, computational, spectroscopic techniques fully characterized the catalytic cycle enzyme. These experiments consistent with mechanism...
Fatty acid photodecarboxylase (FAP) is one of the few enzymes that require light for their catalytic cycle (photoenzymes). FAP was first identified in microalga Chlorella variabilis NC64A, and belongs to an algae-specific subgroup glucose-methanol-choline oxidoreductase family. While from C. its Chlamydomonas reinhardtii homolog CrFAP have demonstrated vitro activities, activities physiological functions not been studied vivo. Furthermore, conservation activity beyond green microalgae...
Ongoing climate change is driving the search for renewable and carbon-neutral alternatives to fossil fuels. Photocatalytic conversion of fatty acids hydrocarbons by acid photodecarboxylase (FAP) represents a promising route green However, alleged low activity FAP on C2 C12 seemed preclude use synthesis gasoline-range hydrocarbons. Here, we reveal that Chlorella variabilis ( Cv FAP) can convert n -octanoic in vitro four times faster than -hexadecanoic acid, its best substrate reported date....
Filamentous phages are widely distributed symbionts of Gram-negative bacteria, with some them being linked to genome evolution and virulence their host. However, the precise mechanism that permits uptake across cell envelope is poorly understood. The canonical phage model Fd requires TolQRA protein complex in host envelope, which suspected translocate protons inner membrane. In this study, we show proceeds presence assembled but nonfunctional complex. Moreover, our results unravel an...
Abstract Fatty Acid Photodecarboxylase (FAP) has emerged as a promising catalyst for the biological production of long-chain hydrocarbons. We have recently shown that purified FAP or FAP-expressing bacteria can efficiently convert octanoic acid into heptane, thus extending potential applications to medium-chain hydrocarbons (i.e., solvent- kerosene-type). The scarcity natural sources presents challenge however. Here, we explore heptane capacity E. coli strain engineered biosynthesize via...