Kyprianos Hadjidemetriou
A5003006917- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Metabolomics and Mass Spectrometry Studies
- Photochromic and Fluorescence Chemistry
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Click Chemistry and Applications
- RNA and protein synthesis mechanisms
- Lipid metabolism and biosynthesis
- Enzyme Catalysis and Immobilization
- Advanced biosensing and bioanalysis techniques
- Algal biology and biofuel production
Institut de Biologie Structurale
2021-2022
Université Grenoble Alpes
2021-2022
CEA Grenoble
2021-2022
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021-2022
Centre National de la Recherche Scientifique
2021-2022
Diamond Light Source
2022
Light makes light work of fatty acids Photosynthetic organisms are notable for their ability to capture energy and use it power biosynthesis. Some algae have gone a step beyond photosynthesis can initiate enzymatic photodecarboxylation acids, producing long-chain hydrocarbons. To understand this transformation, Sorigué et al. brought bear an array structural, computational, spectroscopic techniques fully characterized the catalytic cycle enzyme. These experiments consistent with mechanism...
RsEGFP2 is a reversibly photoswitchable fluorescent protein used in super-resolved optical microscopies, which can be toggled between On state and nonfluorescent Off state. Previous time-resolved ultraviolet-visible spectroscopic studies have shown that the Off-to-On photoactivation extends over femto- to millisecond time scale involves two picosecond lifetime excited states four ground intermediates, reflecting trans-to-cis isomerization, deprotonation, structural reorganizations. Femto-...
Abstract Reversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance novel applications. Here we establish a link between photoswitching contrast, one the key parameters that dictate achievable resolution in nanoscopy applications, chromophore conformation non‐fluorescent state rsEGFP2, widely employed label REversible Saturable OpticaL Fluorescence Transitions...
Upon absorption of a blue-light photon, fatty-acid photodecarboxylase catalyzes the decarboxylation free fatty acids to form hydrocarbons (for example alkanes or alkenes). The major components catalytic mechanism have recently been elucidated by combining static and time-resolved serial femtosecond crystallography (TR-SFX), vibrational electronic spectroscopies, quantum-chemical calculations site-directed mutagenesis [Sorigué et al. (2021), Science, 372, eabd5687]. TR-SFX experiments, which...
Abstract Reversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance novel applications. Here we establish a link between photoswitching contrast, key parameter that largely dictates the achievable resolution in nanoscopy applications, chromophore conformation non-fluorescent state rsEGFP2, widely employed label REversible Saturable OpticaL Fluorescence Transitions...
The Cover Feature displays a comparison of chromophore conformations in the X-ray crystal structures reversibly photoswitchable fluorescent protein rsEGFP2 its on state (green) and V151L (purple) V151A (yellow) variants their off states. latter an increased photoswitching contrast, as indicated by displayed switching kinetics. design Virgile ADAM. More information can be found Research Article Dominique Bourgeois, Martin Weik co-workers.
Time-resolved serial femtosecond crystallography (TR-SFX) at X-ray free electron lasers (XFELs) allows studying the structural dynamics of crystalline biological macromolecules down to sub-picosecond time scale [1].According a pump-probe scheme, optical pump pulses initiate activity in light sensitive proteins and XFEL generate diffraction patterns that allow determining intermediate-state structures.We apply TR-SFX study light-induced reversibly photoswitchable fluorescent protein,...