A5015954127- Orthopedic Infections and Treatments
- Orthopaedic implants and arthroplasty
- Total Knee Arthroplasty Outcomes
- Infective Endocarditis Diagnosis and Management
- Microbial Community Ecology and Physiology
- Infectious Diseases and Tuberculosis
- Microbial Metabolic Engineering and Bioproduction
- Knee injuries and reconstruction techniques
- Hip disorders and treatments
- Surgical site infection prevention
- Nerve injury and regeneration
- Bacterial Identification and Susceptibility Testing
- Biofuel production and bioconversion
- Osteoarthritis Treatment and Mechanisms
- Nerve Injury and Rehabilitation
- Enzyme Catalysis and Immobilization
- Hip and Femur Fractures
- Advancements in Battery Materials
- Advanced Battery Materials and Technologies
- Antifungal resistance and susceptibility
- Mesenchymal stem cell research
- Photoreceptor and optogenetics research
- Optimization and Search Problems
- Orthopedic Surgery and Rehabilitation
- Bone and Joint Diseases
Fujian Medical University
2016-2025
First Affiliated Hospital of Fujian Medical University
2016-2025
Wannan Medical College
2024-2025
Henan University
2025
Regional Medical Center
2024
Putian University
2024
Hebei University
2020-2024
United States Air Force
2024
Nanjing Tech University
2017-2023
Second Affiliated Hospital of Xi'an Jiaotong University
2023
The aim of this study was to evaluate the performance metagenomic next-generation sequencing (mNGS) in detecting pathogens from synovial fluid prosthetic joint infection (PJI) patients.A group 75 patients who underwent revision knee or hip arthroplasties were enrolled prospectively. Ten with primary included as negative controls. Synovial collected for mNGS analysis. Optimal thresholds determined distinguish background microbes. fluid, tissue, and sonicate obtained culture.A total 49 PJI 21...
Periprosthetic joint infection (PJI) diagnosis remains challenging, and the identification of causative microorganism is, by far, most important aspect. Here, we use multiple PCR-based targeted next-generation sequencing (tNGS) to detect pathogens in PJI. To explore 1. ability PJI; 2. consistency tNGS, metagenomic NGS (mNGS), culture results; 3. tNGS drug resistance genes PJI.PJI was diagnosed according Musculoskeletal Infection Society (MSIS) criteria. The microorganisms were detected...
The purpose of this study was to explore the clinical value metagenomic next-generation sequencing (mNGS) in diagnosis polymicrobial periprosthetic joint infection (PJI).Patients with complete data who underwent surgery at our hospital between July 2017 and January 2021 for suspected (PJI), according 2018 ICE diagnostic criteria, were enrolled, all patients microbial culture mNGS detection, which performed on BGISEQ-500 platform. Microbial cultures two samples synovial fluid, six tissue,...
Gastrointestinal stromal tumors (GISTs) are the major gastrointestinal mesenchymal with a variable malignancy ranging from curable disorder to highly malignant sarcomas. Metastasis and recurrence main causes of death in GIST patients. To further explore mechanism metastasis more accurately estimate risk GISTs after surgery, clinical significance functional role collagen triple helix repeat containing-1 (CTHRC1) were investigated. We found that CTHRC1 expression was gradually elevated as...
Abstract Succinic acid is an industrially important platform chemical that could be used as precursor for the establishment of a sustainable industry. Due to depletion crude oil and need development, biological production succinic from renewable resources has attracted wide attention. To establish economical bio‐based process, various metabolic engineering strategies have been developed applied improve strain performance, including efficient CO 2 fixation, by‐product elimination, use...
ObjectivesThe purpose of this study was to explore the role mNGS in diagnosis PJI by detecting presence bacteria periprosthetic tissues.MethodsPatients who were suspected and underwent surgery our center enrolled. Microbial culture performed on tissues collected intraoperatively. Patient age, body mass index (BMI), laboratory tests, microbial results recorded. The diagnostic performance compared.ResultsA total 44 patients included. sensitivity, specificity, positive predictive value (PPV),...
Objectives To evaluate metagenomic next-generation sequencing (mNGS) as a diagnostic tool in detecting pathogens from osteoarticular infection (OAI) samples. Methods 130 samples of joint fluid, sonicate fluid and tissue, were prospectively collected 92 patients with OAI. The performance mNGS microbiology culture was compared pairwise. Results overall sensitivity 88.5% (115/130), significantly higher than microbiological culture, which had 69.2% (90/130, p<0.01). In particular, for (mNGS:...
The aims of this study were to (1) evaluate the efficacy and safety targeted antibiotics for treatment culture-negative prosthetic joint infection based on metagenomic next-generation sequencing results (2) verify accuracy reliability identifying pathogens related infection.Ninety-seven consecutive PJI patients, including 27 patients with infection, treated surgically at our center. Thirteen who admitted before June 2017 empirical antibiotics, comprised antibiotic group (EA group), other 14...
The aims of our study were to (1) evaluate the concordance both methods for detecting prosthetic joint infection (PJI) pathogens in fluid and (2) clarify whether broad-range polymerase chain reaction (BR-PCR) can be used as a verification method metagenomic next-generation sequencing (mNGS) PJI diagnosis.In total, 63 patients underwent total arthroplasty, with 45 18 aseptic failure included. Joint fluids sampled after antibiotics withheld more than 2 weeks, then, culture, BR-PCR mNGS...
Identifying fastidious pathogens in patients with prosthetic joint infection (PJI) by culture is challenging. Metagenomic next-generation sequencing (mNGS) a novel culture-independent approach that associated higher likelihood for identifying pathogens. We present case where mNGS was implemented to identify Parvimonas micra, rarely reported and difficult-to-culture PJI pathogen.
ObjectivesPreoperative diagnosis is important for patients who need revision surgery due to PJI. Microbial culture plays an role in PJI diagnosis, but the sensitivity of cultures low when sample amount limited or a patient treated with antibiotics before collection. In this study, metagenomic next-generation sequencing (mNGS) was used detect bacteria preoperative puncture synovial fluid samples from suspected PJI, and intraoperative results were compared estimate its diagnostic...
This study was to evaluate the safety and effectiveness of carbon nanoparticles suspension in tracking lymph node metastases colorectal cancer.Eligible patients diagnosed with stages I-III cancer Fudan University Shanghai Cancer Center between 1 May 2017 31 2018 fulfilling inclusion criteria were included this prospective randomized controlled study. All randomly allocated two groups: nanocarbon group control group. Patients' clinicopathological characteristics compared For continuous...
To report on our clinical outcomes and the experience of managing acute periprosthetic joint infection (PJI) with debridement, antibiotics, implant retention (DAIR).We performed a retrospective review all patients who were diagnosed PJI after primary hip or knee replacement surgeries managed DAIR in prospective registry from 2008 to 2019. The diagnosis was made according 2011 Musculoskeletal Infection Society (MSIS) criteria. symptom onset duration, inflammatory marker levels (i.e....
This study aimed to explore the diagnostic value of synovial fluid neutrophil extracellular traps (SF-NETs) in periprosthetic joint infection (PJI) diagnosis, and compare it with that microbial culture, serum ESR CRP, white blood cell (WBC) count, polymorphonuclear percentage (PMN%).In a single health centre, patients suspected PJI were enrolled from January 2013 December 2021. The inclusion criteria were: 1) who have PJI; 2) complete medical records; 3) whom sufficient was obtained for...
The efficacy of medial unicompartmental knee arthroplasty (UKA) in patients with intraoperatively identified patellofemoral osteoarthritis (PFOA) has been a subject debate. This retrospective study aimed to investigate the early outcomes UKA varying intraoperative PFOA conditions and explore relationship between location position prosthesis post-UKA. Our aim was determine whether presence affects short-term success UKA. single-center, included who underwent by same surgical team from March...
Abstract The interaction between the nervous system and immune during chronic bacterial infection remains unclear. Here, it is reported that Staphylococcus aureus ( S. ) induces calcitonin gene‐related peptide (CGRP) secretion from intra‐articular transient receptor potential cation channel subfamily V member 1 positive (TRPV1 + nociceptive nerves through its pore‐forming toxin (PFT) α‐hemolysin. released CGRP then inhibits production of chemotactic cytokines by CX3CR1 tissue‐resident...
Two‐dimensional covalent organic frameworks (2DCOFs) are a unique class of crystalline porous materials interconnected by bonds, which have attracted significant attention in recent years due to their chemical and structural diversity, as well applications adsorption, separation, catalysis, drug delivery. However, research on the electrical properties 2DCOFs remains limited, despite potential electronics. Early studies recognized poor conductivity obstacle application this field. To overcome...