A5009463488- Neuroinflammation and Neurodegeneration Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Proteoglycans and glycosaminoglycans research
- Sleep and related disorders
- Regulation of Appetite and Obesity
- Silk-based biomaterials and applications
- PARP inhibition in cancer therapy
- S100 Proteins and Annexins
- DNA Repair Mechanisms
- Cannabis and Cannabinoid Research
- Toxin Mechanisms and Immunotoxins
- Genomics and Chromatin Dynamics
- Signaling Pathways in Disease
- Antimicrobial Peptides and Activities
- Sleep and Wakefulness Research
- HIV/AIDS drug development and treatment
- Liver Disease Diagnosis and Treatment
- Immune cells in cancer
- Epigenetics and DNA Methylation
Centre National de la Recherche Scientifique
2016-2024
Institut Gustave Roussy
2024
Université Paris-Saclay
2024
Sorbonne Université
2014-2021
Inserm
2014-2021
Institut du Cerveau
2014-2021
Université Paris Cité
2021
Université Paris-Est Créteil
2017-2020
Department of Virology
2020
Institut National de Recherche en Santé Publique
2020
Abstract Pediatric high-grade gliomas (pHGG) are devastating and incurable brain tumors with recurrent mutations in histone H3.3. These promote oncogenesis by dysregulating gene expression through alterations of modifications. We identify aberrant DNA repair as an independent mechanism, which fosters genome instability H3.3 mutant pHGG, opens new therapeutic options. The two most frequent K27M G34R, drive replication-associated damage non-homologous end joining (NHEJ). Aberrant NHEJ is...
Cyclophilins play a key role in the life cycle of coronaviruses. Alisporivir (Debio 025) is nonimmunosuppressive analogue cyclosporine with potent cyclophilin inhibition properties. reduced SARS-CoV-2 RNA production dose-dependent manner Vero E6 cells, 50% effective concentration (EC 50 ) 0.46 ± 0.04 μM. inhibited postentry step cycle. These results justify rapidly conducting proof-of-concept phase 2 trial alisporivir patients infection.
Aggregated forms of the synaptic protein α-synuclein (αS) have been proposed to operate as a molecular trigger for microglial inflammatory processes and neurodegeneration in Parkinson´s disease. Here, we used brain cell cultures activated by fibrillary recombinant human αS assess anti-inflammatory neuroprotective activities antibiotic rifampicin (Rif) its autoxidation product quinone (RifQ). Pretreatments with Rif RifQ reduced secretion prototypical cytokines (TNF-α, IL-6) burst oxidative...
Abstract When activated, microglial cells have the potential not only to secrete typical proinflammatory mediators but also release neurotransmitter glutamate in amounts that may promote excitotoxicity. Here, we wished determine of Parkinson's disease (PD) protein α‐Synuclein (αS) stimulate using cultures purified cells. We established was robustly increased when were treated with fibrillary aggregates αS native monomeric protein. Promotion by (αSa) required concomitant engagement TLR2 and...
Dermaseptin-B2 (DRS-B2) is a multifunctional cationic antimicrobial peptide (CAP) isolated from frog skin secretion. We previously reported that DRS-B2 possesses anticancer and antiangiogenic activities in vitro vivo. In the present study, we evaluated antiproliferative activity of on numerous tumor cell lines, its internalization studies molecular partners as well their influences structure. Confocal microscopy using ([Alexa594]-(Cys0)-DRS-B2) shows sensitive human cells (PC3), seems to...
Our present objective was to better characterize the mechanisms that regulate striatal neuroinflammation in mice developing L-DOPA-induced dyskinesia (LID). For that, we used 6-hydroxydopamine (6-OHDA)-lesioned rendered dyskinetic by repeated intraperitoneal injections of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) and quantified ensuing neuroinflammatory changes dopamine-denervated dorsal striatum. LID development associated with a prominent astrocytic response, more moderate microglial cell...
Purified microglial cells in culture are frequently used to model brain inflammatory responses but obtaining large yields of these on a routine basis can be quite challenging. Here, we demonstrate that it is possible achieve high‐yield isolation pure (MAC‐1 + /Fcrls /Ccr2 ‐ ) from postnatal tissue through simple procedure mainly relies the adhesion preference polycation polyethyleneimine (PEI) serum‐supplemented DMEM medium. Accordingly, other synthetic or biological substrates failed mimic...
To determine whether orexinergic hypothalamic peptides can influence the survival of brainstem dopamine (DA) neurons, we used a model system rat midbrain cultures in which DA neurons degenerate spontaneously and progressively as they mature. We established that orexin (OX)-B provides partial but significant protection to dying whereas homologous peptide OXA has only marginal effects. Importantly, rescued by OXB accumulated efficiently active transport, suggesting were functional....
Summary Background In hepatitis C virus (HCV) infection, treatment failure is generally associated with the selection of resistance‐associated substitutions (RAS) conferring reduced susceptibility to direct‐acting antiviral (DAA) drugs. Resistant variants continue replicate after end potential for transmission. This may result from “fitness‐associated substitutions”. Aim To characterise substitutions” in patients infected genotype 3a failing DAA drugs Methods By means shotgun metagenomics,...
The quinoline MK-571 is the most commonly used inhibitor of multidrug resistance protein-1 (MRP-1) but was originally developed as a cysteinyl leukotriene receptor 1 (CysLTR1) antagonist. While studying modulatory effect MRP-1 on anti-hepatitis C virus (HCV) direct-acting antiviral (DAA) efficiency, we observed an unexpected anti-HCV compound alone. This activity characterized in Huh7.5 cells stably harboring subgenomic genotype 1b replicon. A dose-dependent decrease HCV RNA levels upon...