Uropathogenic Escherichia coli (UPEC) strains isolated from patients with community-acquired urinary tract infections (UTIs) were assessed to determine the prevalence of virulence genes, antibiotic resistance, and O-serogroup strains.Consenting UTI enrolled at Unidad Médica Familiar Number 64 (Instituto Mexicano del Seguro Social, Estado de Mexico, Mexico) 321 urine samples collected. Polymerase chain reaction (PCR) was used assess 24 genes 14 O-serogroups. The Kirby-Bauer method evaluate...

Abstract Background Diarrhoeal diseases constitute a major public health problem, particularly in the developing world, where rate of mortality and morbidity is very high. The purpose this study was to conduct 2 years 3 months order determine prevalence five enteropathogen diarrheogenic agents Mexico City. Methods Faecal samples were obtained from 300 Mexican children diagnosed as positive for diarrhoea, aged > < 12 old, 80 matched age but with no symptoms disease (control group). Two...

Abstract Background Helicobacter pylori has been strongly associated with chronic gastritis, peptic and duodenal ulcers, it is a risk factor for gastric cancer. Three major virulence factors of H. have described: the vacuolating toxin (VacA), cytotoxin-associated gene product (CagA) adhesion protein BabA2. Since considerable geographic diversity in prevalence reported, aim this work was to establish vacA , cagA babA2 status 238 adult patients, from marginal urban area Mexico, gastritis....

The aim of this study was to characterize the Staphylococcus aureus strains isolated from periodontal lesions patients, determine expression genes involved in cell adhesion upon their infection human epithelial cells using an vitro model, its biofilm formation, and resistance antibiotics. S. analysed by PCR, Kirby–Bauer, pulsed-field gel electrophoresis (PFGE), measuring gene real-time PCR after vitro. identified 18.6% (50/268) samples. All (n = 50) possessed virulence spa (Staphylococcal...

Antifungal resistance and virulence properties of Candida albicans are a growing health problem worldwide. To study the expression azole genes in 39 clinical strains C. , we used model infection human vaginal epithelial cells with isolated from Mexican women vulvovaginal candidiasis (VVC). The were identified by PCR amplification ITS1 ITS2 regions rRNA. detection MDR1 CDR1 performed using RT-PCR, respectively. All sensitive to nystatin 38 (97.4%) 37 (94.9%) resistant ketoconazole...

Virulence and antibiotic resistance properties related to different Escherichia coli phylogenetic groups have not been studied in detail Mexico. We aimed identify patterns of virulence genes multidrug uropathogenic strains (UPEC). Strains E. were isolated from outpatients with urinary tract infections (UTIs), who went unit the public health sector State markers identified by PCR. Susceptibility 12 antimicrobials was determined Kirby-Bauer. 60.4% (n = 194) patients UTIs. Phylogroups B2 51%...

Methicillin-resistant Staphylococcus aureus is an increasingly important cause of nosocomial bacterial infections worldwide. S. responsible for several pathologies, including skin infections, endocarditis, meningitis, deep-tissue ulcers, and sepsis. biofilm formation on catheters other medical devices a major post-operative concern, because biofilms are often the source persistent difficult to treat infections. While catheter-related have been reported, strains these not genetically...

Abstract Pasteurella multocida (Pm) is a gram‐negative bacterium able to infect different animal species, including human beings. This causes economic losses the livestock industry because of its high morbidity and mortality in animals. In this work, we report characterization outer membrane vesicles (OMVs) released into culture medium by Pm serogroups. Purified OMVs range 50–300 nm were observed electron microscopy. Serum obtained from chickens infected with recognized several proteins...

To detect the frequency and expression of eight ALS (agglutinin-like sequence) genes HWP1 genotype in a group Candida albicans strains isolated from Mexican women suffering vaginal candidosis. A 264 (age 15-57 years) with infections were evaluated. C. was identified by PCR amplification rRNA internal transcribed spacer regions ITS1 ITS2. The conventional PCR, their levels determined real-time after growing reconstituted human epithelium (RHVE). 50 (18.9%). genotypic frequencies ALS1 100%,...

The pathogenicity of Escherichia coli strains that cause cervico-vaginal infections (CVI) is due to the presence several virulence genes. objective this study was define variability regarding genotype antibiotic resistance, transcription profiles genes after in vitro infection vaginal cell line A431 and phylogroup composition a group E. (CVEC). A total 200 isolated from Mexican women with CVI two medical units Institute Social Security were analysed. resistance identified using conventional...

Gallibacterium anatis (previously named Pasteurella haemolytica-like) is considered a normal inhabitant of genital and upper respiratory tracts healthy chickens, but it also associated with different pathological conditions. Secreted metalloproteases from field reference G. cultures were obtained by methanol precipitation characterized. Proteins molecular mass higher than 100 kDa showing proteolytic activity observed in 10% polyacrylamide gels copolymerized 1% bovine casein. They active at...

To determine the frequency and expression of ten SAP (secreted aspartyl protease) genes in a group Candida albicans strains isolated from Mexican women suffering vaginal candidosis, 264 (age 18 - 57 years) with infections, predisposed by diabetes mellitus or contraceptive consumption, were evaluated. C. was identified using PCR to amplify rRNA internal transcribed spacer regions ITS1 ITS2. The presence determined conventional PCR, their levels real-time after had been grown reconstituted...

Background:Escherichia coli is the main bacterium associated with urinary tract infections (UTIs), including cystitis and pyelonephritis. Uropathogenic E. (UPEC) harbors numerous genes that encode diverse virulence factors contributing to its pathogenicity. The treatment of UTIs has become complicated due natural selection strains are multiresistant several groups antibiotics regularly used in clinical settings such as hospitals. Genomic reports global composition distribution antibiotic...

ABSTRACT Actinobacillus pleuropneumoniae has been considered nonmotile and nonflagellate. In this work, it is demonstrated that A. produces flagella composed of a 65-kDa protein with an N-terminal amino acid sequence shows 100% identity those Escherichia coli , Salmonella Shigella flagellins. The DNA obtained through PCR the fliC gene in showed considerable (93%) its 5′ 3′ ends sequences corresponding genes E. enterica spp. motility was observed tryptic soy or brain heart infusion soft agar...

Microbial infections are typically initiated by the colonization of tissues a specific mechanism that promotes adherence to host cells or tissues. In this work, we characterized ability Gallibacterium anatis F149T express fimbriae may be involved in mucosal attachment. Using transmission electron microscopy, fimbriae-like structures could observed on surface negatively stained G. F149T, and these were further visualized after being released physical shaking. When separated SDS-PAGE, proteins...

Methicillin-resistant Staphylococcus aureus (MRSA) are clinically relevant pathogens that cause severe catheter-related nosocomial infections driven by several virulence factors. We implemented a novel model of infection in vitro reconstituted human epithelium (RHE) to analyze the expression patterns genes 21 MRSA strains isolated from Mexican patients undergoing haemodialysis. also determined phenotypic and genotypic co-occurrence antibiotic- disinfectant-resistance traits S. strains, which...

Avibacterium paragallinarum, the causative agent of infectious coryza, releases extracellular membrane vesicles (MVs), containing immunogenic proteins, proteases, putative RTX haemagglutinin, and nucleic acids, into medium. MVs ranging 50-300 nm in diameter were observed by electron microscopy. They contained proteins range 20-160 kDa, detected using vaccinated or experimentally infected chicken sera raised against Av. but not pooled from specific pathogen-free chickens. Proteolytic activity...

Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH 4 ) 2 SO or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized porcine gelatin (0.1%). They were active a broad pH range (4–9); 7.5 being the optimum. Protease was inhibited 20 mmol EDTA/L and reactivated calcium. The heat-stable 40, 50, 60 °C, but its...