Respiratory diseases in ruminants are a main cause of economic losses to farmers worldwide. Approximately 25% experience at least one episode respiratory disease during the first year life. Mannheimia haemolytica is etiological bacterial agent ruminant complex. M. can secrete several virulence factors, such as leukotoxin, lipopolysaccharide, and proteases, that be targeted treat infections. At present, little information has been reported on secretion A2 proteases their host protein targets....

Actinobacillus pleuropneumoniae serotype 1 releases vesicles containing proteases and Apx toxins into the culture medium. Vesicles were concentrated by ultracentrifugation analyzed electron microscopy electrophoresis; their size ranged from 20 to 200 nm. A polyclonal antiserum raised against a purified high molecular mass secreted protease of recognized this on surface immunogold microscopy. Higher polypeptides vesicle extracts Western immunoblot, indicating that could form oligomers....

Methicillin-resistant Staphylococcus aureus is an increasingly important cause of nosocomial bacterial infections worldwide. S. responsible for several pathologies, including skin infections, endocarditis, meningitis, deep-tissue ulcers, and sepsis. biofilm formation on catheters other medical devices a major post-operative concern, because biofilms are often the source persistent difficult to treat infections. While catheter-related have been reported, strains these not genetically...

Abstract Pasteurella multocida (Pm) is a gram‐negative bacterium able to infect different animal species, including human beings. This causes economic losses the livestock industry because of its high morbidity and mortality in animals. In this work, we report characterization outer membrane vesicles (OMVs) released into culture medium by Pm serogroups. Purified OMVs range 50–300 nm were observed electron microscopy. Serum obtained from chickens infected with recognized several proteins...

To detect the frequency and expression of eight ALS (agglutinin-like sequence) genes HWP1 genotype in a group Candida albicans strains isolated from Mexican women suffering vaginal candidosis. A 264 (age 15-57 years) with infections were evaluated. C. was identified by PCR amplification rRNA internal transcribed spacer regions ITS1 ITS2. The conventional PCR, their levels determined real-time after growing reconstituted human epithelium (RHVE). 50 (18.9%). genotypic frequencies ALS1 100%,...

Gallibacterium anatis (previously named Pasteurella haemolytica-like) is considered a normal inhabitant of genital and upper respiratory tracts healthy chickens, but it also associated with different pathological conditions. Secreted metalloproteases from field reference G. cultures were obtained by methanol precipitation characterized. Proteins molecular mass higher than 100 kDa showing proteolytic activity observed in 10% polyacrylamide gels copolymerized 1% bovine casein. They active at...

To determine the frequency and expression of ten SAP (secreted aspartyl protease) genes in a group Candida albicans strains isolated from Mexican women suffering vaginal candidosis, 264 (age 18 - 57 years) with infections, predisposed by diabetes mellitus or contraceptive consumption, were evaluated. C. was identified using PCR to amplify rRNA internal transcribed spacer regions ITS1 ITS2. The presence determined conventional PCR, their levels real-time after had been grown reconstituted...

ABSTRACT Actinobacillus pleuropneumoniae has been considered nonmotile and nonflagellate. In this work, it is demonstrated that A. produces flagella composed of a 65-kDa protein with an N-terminal amino acid sequence shows 100% identity those Escherichia coli , Salmonella Shigella flagellins. The DNA obtained through PCR the fliC gene in showed considerable (93%) its 5′ 3′ ends sequences corresponding genes E. enterica spp. motility was observed tryptic soy or brain heart infusion soft agar...

Microbial infections are typically initiated by the colonization of tissues a specific mechanism that promotes adherence to host cells or tissues. In this work, we characterized ability Gallibacterium anatis F149T express fimbriae may be involved in mucosal attachment. Using transmission electron microscopy, fimbriae-like structures could observed on surface negatively stained G. F149T, and these were further visualized after being released physical shaking. When separated SDS-PAGE, proteins...

Methicillin-resistant Staphylococcus aureus (MRSA) are clinically relevant pathogens that cause severe catheter-related nosocomial infections driven by several virulence factors. We implemented a novel model of infection in vitro reconstituted human epithelium (RHE) to analyze the expression patterns genes 21 MRSA strains isolated from Mexican patients undergoing haemodialysis. also determined phenotypic and genotypic co-occurrence antibiotic- disinfectant-resistance traits S. strains, which...

The complete amino acid and nucleotide sequence of a secreted metalloprotease produced by Actinobacillus pleuropneumoniae serotype 1 is reported. A clone showing proteolytic activity in cell-free culture media was selected from genomic library A. pUC 19. obtained contained an open reading frame encoding protein with 869 acids. This identified as zinc neutral-metalloprotease belonging to the aminopeptidase family, predicted molecular weight approximately 101 kDa. showed high homology other or...

In this study, we investigated the reverse transcriptase subunit of telomerase in dimorphic fungus Ustilago maydis. This protein (Trt1) contains 1371 amino acids and all characteristic TERT motifs. Mutants created by disrupting trt1 had senescent traits, such as delayed growth, low replicative potential, reduced survival, that were reminiscent traits observed est2 budding yeast mutants. Telomerase activity was wild-type sporidia but not those disruption mutant. The introduction a...

Haemophilus paragallinarum is the causal agent of infectious coryza, an economically important disease for poultry industry. This bacterium secreted proteins 25-110 kDa during its growth in brain heart infusion, tryptic soy broth, or Luria-Bertani glucose phosphate media, all lacking serum. Some these were recognized by sera from chickens experimentally infected with H. paragallinarum. A 110-kDa protein was a serum pool convalescent-phase pigs naturally Actinobacillus pleuropneumoniae, and...

Avibacterium paragallinarum, the causative agent of infectious coryza, releases extracellular membrane vesicles (MVs), containing immunogenic proteins, proteases, putative RTX haemagglutinin, and nucleic acids, into medium. MVs ranging 50-300 nm in diameter were observed by electron microscopy. They contained proteins range 20-160 kDa, detected using vaccinated or experimentally infected chicken sera raised against Av. but not pooled from specific pathogen-free chickens. Proteolytic activity...