A5075242803- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Insect symbiosis and bacterial influences
- HIV Research and Treatment
- Vector-borne infectious diseases
- Biosensors and Analytical Detection
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- RNA Interference and Gene Delivery
- Insect Resistance and Genetics
- Monoclonal and Polyclonal Antibodies Research
- Infectious Encephalopathies and Encephalitis
- Endoplasmic Reticulum Stress and Disease
- Virology and Viral Diseases
- Lipid Membrane Structure and Behavior
- Nuclear Structure and Function
- Vibrio bacteria research studies
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Dengue and Mosquito Control Research
- Parasites and Host Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Parasitic Diseases Research and Treatment
National Science and Technology Development Agency
2016-2025
National Center for Genetic Engineering and Biotechnology
2016-2025
Siriraj Hospital
2015-2025
Mahidol University
2015-2025
Dengue virus co-circulates as four serotypes, and sequential infections with more than one serotype are common. One hypothesis for the increased severity seen in secondary is antibody-dependent enhancement (ADE) leading to replication Fc receptor-bearing cells. In this study, we have generated a panel of human monoclonal antibodies dengue virus. Antibodies structural precursor-membrane protein (prM) form major component response. These highly cross-reactive among serotypes and, even at high...
Vascular leakage and shock are the major causes of death in patients with dengue hemorrhagic fever (DHF) syndrome (DSS). Thirty years ago, complement activation was proposed to be a key underlying event, but cause has remained unknown.The nonstructural virus (DV) protein NS1 tested for its capacity activate human membrane-associated soluble forms. Plasma samples from 163 DV infection 19 other febrile illnesses were prospectively analyzed viral load levels complement-activation products....
Dengue virus (DENV) nonstructural protein-1 (NS1) is a secreted glycoprotein that absent from viral particles but accumulates in the supernatant and on plasma membrane of cells during infection. Immune recognition cell surface NS1 endothelial has been hypothesized as mechanism for vascular leakage occurs severe DENV However, it remained unclear how becomes associated with membrane, contains no membrane-spanning sequence motif. Using flow cytometric ELISA-based binding assays mutant lines...
ABSTRACT During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation cryoelectron microscopy revealed third type particles, partially as major prM-containing particles in serotype 2 virus. Changes proportion viral pr-M junction mutants exhibiting altered levels prM suggest that may represent intermediate subpopulation...
ABSTRACT Flavivirus nonstructural protein 2A (NS2A) plays important roles in both viral RNA synthesis and virion assembly. The molecular details of how the NS2A modulates two distinct events have not been defined. To address this question, we performed a systematic mutagenesis using dengue virus (DENV) serotype 2 (DENV-2) as model. We identified sets mutations with defects during infection cycle. One set (D125A G200A) selectively abolished synthesis. Mechanistically, D125A mutation through...
In the generation of flavivirus particles, an internal cleavage envelope glycoprotein prM by furin is required for acquisition infectivity. Unlike other flaviviruses, dengue virus incomplete in many cell lines; partial reflects influence residues at nonconsensus positions pr-M junction, as flaviviruses share basic P1, P2, and P4, recognized furin. this study, viruses harboring alanine-scanning multiple-point mutations junction were generated, employing a background that exhibited 60 to 70%...
Abstract Flavivirus nonstructural protein 1 (NS1) is a unique secreted glycoprotein. Although it absent from the flavivirus virion, intracellular and extracellular forms of NS1 have essential roles in viral replication pathogenesis infection. The fate insect cells has been more controversial, with some reports suggesting exclusively cell associated. In this study, we confirm secretion origin characterize its physical, biochemical, functional properties context dengue virus (DENV) Unlike...
ABSTRACT During the export of flavivirus particles through secretory pathway, a viral envelope glycoprotein, prM, is cleaved by proprotein convertase furin; this cleavage required for subsequent rearrangement receptor-binding E glycoprotein and virus infectivity. Similar to many furin substrates, prM in vector-borne flaviviruses contains basic residues at positions P1, P2, P4 proximal site; addition, number charged are found position P3 between P5 P13 that conserved each antigenic complex....
During the replication of dengue virus, a viral non-structural glycoprotein, NS1, associates with membrane on cell surface and in RNA complex. NS1 lacks transmembrane domain, mechanism by which it remains unclear. This study aimed to investigate whether membrane-bound is present lipid rafts virus-infected cells. Double immunofluorescence staining infected HEK-293T cells revealed that localized raft-associated molecules, ganglioside GM1 CD55, surface. In flotation gradient centrifugation...
During infection, the capsid (C) protein of many flaviviruses localizes to nuclei and nucleoli several infected cell lines; underlying basis significance C nuclear localization remain poorly understood. In this study, double alanine-substitution mutations were introduced into three previously proposed nuclear-localization signals (at positions 6-9, 73-76 85-100) dengue virus protein, four viable mutants, c(K6A,K7A), c(K73A,K74A), c(R85A,K86A) c(R97A,R98A), generated in a mosquito line which...
Abstract Background Dengue is the most significant mosquito-borne viral disease; there are no specific therapeutics. The antiparasitic drug ivermectin efficiently inhibits replication of all 4 dengue virus serotypes in vitro. Methods We conducted 2 consecutive randomized, double-blind, placebo-controlled trials adult patients to evaluate safety and virological clinical efficacies ivermectin. After a phase trial with or 3 days 1 daily dose 400 µg/kg ivermectin, we continued 3, Results showed...
A range of biosensing techniques including immunoassays are routinely used for quantitation analytes in biological samples and available a formats, from centralized lab testing (e.g., microplate enzyme-linked immunosorbent assay (ELISA)) to automated point-of-care (POC) lateral flow immunochromatographic tests. High analytical performance is intrinsically linked the use sequence reagent washing steps, yet this extremely challenging deliver at POC without high level fluidic control involving,...
Dengue is a mosquito-borne disease caused by dengue virus (DENV) infection, which remains major public health concern worldwide owing to the lack of specific treatments or antiviral drugs available. This study investigated potential repurposing domperidone, an antiemetic and gastrokinetic agent, control DENV infection. Domperidone was identified pharmacophore-based virtual screening as small molecule that can bind both viral envelope (E) nonstructural protein 1 (NS1) DENV. Molecular dynamics...
Here we present an approach that advances the throughput of a genetic analysis positive-sense RNA virus by simplifying construction. It enabled comprehensive dissection complex, multigene phenotype through rapid derivation large number chimeric viruses and construction mutant library directly from pool. The versatility described here expands applicability diverse approaches to study these viruses.
Host and viral proteins are involved in dengue virus (DENV) replication. Heterogeneous ribonucleoprotein (hnRNP) C1/C2 abundant host cellular that exhibit RNA binding activity play important roles the replication of positive-strand viruses such as poliovirus hepatitis C virus. hnRNP have previously been shown to interact with vimentin NS1 DENV-infected cells; however, their functional role DENV is not clearly understood. In present study, we investigated by using an vitro model infection a...
Abstract Viruses manipulate the life cycle in host cells via use of viral properties and machineries. Development antiviral peptides against dengue virus (DENV) infection has previously been concentrated on blocking actions structural proteins enzymes entry RNA processing cells. In this study, we proposed DENV NS1, which is a multifunctional non-structural protein indispensable for production, as new target inhibition by specific peptides. We performed biopanning assays using phage-displayed...