A5012401221- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Congenital heart defects research
- Wnt/β-catenin signaling in development and cancer
- Cellular transport and secretion
- Nerve injury and regeneration
- Developmental Biology and Gene Regulation
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Retinal Development and Disorders
- Neuroscience and Neural Engineering
- Adipose Tissue and Metabolism
- Axon Guidance and Neuronal Signaling
- RNA modifications and cancer
- Exercise and Physiological Responses
- Neurogenesis and neuroplasticity mechanisms
- Pain Mechanisms and Treatments
Weizmann Institute of Science
2018-2023
How is protein synthesis initiated locally in neurons? We found that mTOR (mechanistic target of rapamycin) was activated and then up-regulated injured axons, owing to local translation messenger RNA (mRNA). This mRNA transported into axons by the cell size-regulating RNA-binding nucleolin. Furthermore, controlled axons. included regulation its own retrograde injury signaling molecules such as importin β1 STAT3 (signal transducer activator transcription 3). Deletion 3' untranslated region...
How is neuropathic pain regulated in peripheral sensory neurons? Importins are key regulators of nucleocytoplasmic transport. In this study, we found that importin α3 (also known as karyopherin subunit alpha 4) can control responsiveness neurons mice. Importin knockout or neuron-specific knockdown mice reduced to diverse noxious stimuli and increased tolerance pain. α3-bound c-Fos α3-deficient were impaired nuclear import. Knockdown dominant-negative inhibition c-Jun silico screens...
Nucleolin is a multifunctional RNA Binding Protein (RBP) with diverse subcellular localizations, including the nucleolus in all eukaryotic cells, plasma membrane tumor and axon neurons. Here we show that glycine arginine rich (GAR) domain of nucleolin drives localization via protein-protein interactions kinesin light chain. In addition, GAR sequences mediate nucleolin. Both these modalities are addition to already reported involvement liquid-liquid phase separation nucleolus. transport axons...
Polypyrimidine tract binding protein 1 (PTBP1) is thought to be expressed only at embryonic stages in central neurons. Its down-regulation triggers neuronal differentiation precursor and non-neuronal cells, an approach recently tested for generation of neurons de novo amelioration neurodegenerative disorders. Moreover, PTBP1 replaced by its paralog PTBP2 mature Unexpectedly, we found that both proteins are coexpressed adult sensory motor neurons, with restricted mainly the nucleus, while...
The cytoplasmic dynein motor complex transports essential signals and organelles from the cell periphery to perinuclear region, hence is critical for survival function of highly polarized cells such as neurons. Dynein Light Chain Roadblock-Type 1 (DYNLRB1) thought be an accessory subunit required specific cargos, but here we show that it general dynein-mediated transport sensory neuron survival. Homozygous Dynlrb1 null mice are not viable die during early embryonic development. Furthermore,...
Size homeostasis is a fundamental process in biology and particularly important for large cells such as neurons. We previously proposed motor-dependent length-sensing mechanism wherein reductions microtubule motor levels would be expected to accelerate neuronal growth, validated this prediction dynein heavy chain 1 Loa mutant (Dync1h1Loa) sensory Here, we describe new mouse model with conditional deletion allele of exons 24 25 Dync1h1. Homozygous Islet1-Cre-mediated Dync1h1...
ABSTRACT First Person is a series of interviews with the first authors selection papers published in Journal Cell Science, helping researchers promote themselves alongside their papers. Agostina Di Pizio author on ‘ A conditional null allele Dync1h1 enables targeted analyses dynein roles neuronal length sensing’, JCS. PhD student lab Prof. Mike Fainzilber at Weizmann Institute Rehovot, Israel, investigating growth and regeneration.
ABSTRACT The cytoplasmic dynein motor complex transports essential signals and organelles from the cell periphery to perinuclear region, hence is critical for survival function of highly polarized cells such as neurons. Dynein Light Chain Roadblock-Type 1 (DYNLRB1) thought be an accessory subunit required specific cargos, but here we show that it general dynein-mediated transport sensory neuron survival. Homozygous Dynlrb1 null mice are not viable die during early embryonic development....
Abstract Size homeostasis is one of the most fundamental aspects biology and it particularly important for large cells as neurons. We have previously proposed a motor-dependent length-sensing growth-regulating mechanism wherein partial reduction in levels microtubule motor proteins should lead to accelerated neuronal growth. This prediction was originally validated sensory neurons heterozygous Loa point mutation dynein heavy chain 1 ( Dync1h1 ). Here we describe new mouse model with...