A5054528009- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- Hereditary Neurological Disorders
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Plant Reproductive Biology
- Plant and Biological Electrophysiology Studies
- RNA regulation and disease
- Hippo pathway signaling and YAP/TAZ
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hedgehog Signaling Pathway Studies
- Platelet Disorders and Treatments
- Nuclear Structure and Function
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Heat shock proteins research
- CRISPR and Genetic Engineering
- Vestibular and auditory disorders
- Genetic Neurodegenerative Diseases
- Blood properties and coagulation
- interferon and immune responses
- Trace Elements in Health
- Ion Channels and Receptors
- Drug Transport and Resistance Mechanisms
- Hearing, Cochlea, Tinnitus, Genetics
Kongju National University
2020-2023
Korea Advanced Institute of Science and Technology
2005-2020
Chungnam National University
2016
Seoul National University
2008
Dongwha Pharm (South Korea)
2004
Hearing in Drosophila depends on the transduction of antennal vibration into receptor potentials by ciliated sensory neurons Johnston's organ, chordotonal organ. We previously found that a protein vanilloid subfamily (TRPV) channel subunit, Nanchung (NAN), is localized to cilia and required generate sound-evoked (Kim et al., 2003). Here, we show only other TRPV mutated behavioral mutant inactive ( iav ). The IAV forms hypotonically activated when expressed cultured cells; flies, it...
In Drosophila melanogaster, specification of wing vein cells and sensory organ precursor (SOP) cells, which later give rise to a bristle, requires EGFR signaling. Here, we show that Pumilio (Pum), an RNA-binding translational repressor, negatively regulates signaling in bristle development. We observed loss Pum function yielded extra veins additional bristles. Conversely, overexpression eliminated Heterozygotes for produced no phenotype on their own, but greatly enhanced phenotypes caused by...
The Drosophila eye-antenna imaginal disc (ead) is a flattened sac of two-layered epithelia, from which most head structures are derived. Secreted morphogens like Wingless (Wg), Hedgehog (Hh), and Decapentaplegic (Dpp) important for early patterning ead, but the underlying mechanisms still largely unknown. To understand how these function in ead larval stages, we used wg-LacZ dpp-Gal4 markers examination wild-type mutant eads. We found that immediately after hatching was crescent-shaped with...
Abstract The promyelocytic leukemia zinc finger (PLZF) protein has been described as a transcriptional repressor of the BTB‐domain/zinc‐finger family, and shown to regulate expression Hox genes during embryogenesis cyclin A in cell cycle progression. Here, 45‐kDa isoform PLZF without BTB domain was identified via yeast two‐hybrid screening using C‐terminal region ATP7B bait our determination biological roles Wilson disease outside its copper‐binding domain. Our immunoprecipitation...
Rab40 proteins are an atypical subgroup of Rab GTPases containing a unique suppressor the cytokine signaling (SOCS) domain that is recruited to assemble CRL5 E3 ligase complex for proteolytic regulation in various biological processes.A nonsense mutation deleting C-terminal SOCS box RAB40B gene was identified family with axonal peripheral neuropathy (Charcot-Marie-Tooth disease type 2), and pathogenicity assessed model organisms zebrafish Drosophila.Compared control fish, larvae transformed...
In Drosophila, broad complex, tramtrack, bric à brac (BTB)/poxvirus and zinc finger (POZ) transcription factors are essential regulators of development. We searched the Drosophila genome for BTB/POZ-ZF domains discovered an unknown gene, dPLZF, which encodes orthologue human PLZF. then characterized biological function dPLZF via genetic interaction analysis. Ectopic expression in wing induced extra vein formation during development Drosophila. Genetic interactions between Ras or...
Charcot-Marie-Tooth disease (CMT) and related diseases are a genetically clinically heterogeneous group of peripheral neuropathies. Particularly, mutations in several aminoacyl-tRNA synthetase (ARS) genes have been reported to cause axonal CMT (CMT2) or distal hereditary motor neuropathy (dHMN). However, the common pathogenesis among subtypes by different ARS gene defects is not well understood. This study was performed investigate cohort 710 Korean families. Whole-exome sequencing applied...
Small heat shock proteins (sHSPs) are ATP-independent chaperones that help correct the folding of denatured and protect cells from stress. Mutations in HSPB1, HSPB8, HSPB3 implicated inherited peripheral neuropathies (IPNs), such as Charcot-Marie-Tooth disease type 2 (CMT2) distal hereditary motor (dHMN). This study, using whole exome sequencing or targeted gene sequencing, identified 9 pathogenic likely variants these three sHSP genes 11 Korean IPN families. Most were located evolutionally...
Abstract Dr osophila Lobe (L) alleles were first discovered ∼100 years ago as spontaneous dominant mutants with characteristic developmental eye defects. However, the molecular basis for L phenotypes has not been clearly understood. A previous work reported identification of CG10109/PRAS40 gene, but subsequent analyses suggested that PRAS40 may be related to L. Here, we revisited gene clarify this discrepancy and understand dominance mutations. Genetic analysis localized Oaz, which encodes a...
CRISPR mutagenesis is an efficient way to disrupt specific target genes in many model organisms. We previously devised a targeted recombination method generate intragenic recombinants of alleles Drosophila. Here, we assessed the applicability targeting-induced different genetic loci. compared ectopic rates male germline by targeting at two neighboring loci within genomic region that consists repressed chromatin domain Lobe gene, and transcriptionally active PRAS40. Targeting around...