A5088590453- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Anesthesia and Sedative Agents
- Proteoglycans and glycosaminoglycans research
- Patient Safety and Medication Errors
- Pharmaceutical studies and practices
- Mechanical Circulatory Support Devices
- Heparin-Induced Thrombocytopenia and Thrombosis
- Lymphoma Diagnosis and Treatment
- Anesthesia and Neurotoxicity Research
- Viral gastroenteritis research and epidemiology
- Acute Myocardial Infarction Research
- Intensive Care Unit Cognitive Disorders
- Immunotherapy and Immune Responses
- Cardiac Arrest and Resuscitation
- Cystic Fibrosis Research Advances
- Clostridium difficile and Clostridium perfringens research
- Biochemical and Molecular Research
- Inhalation and Respiratory Drug Delivery
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Metabolism and Genetic Disorders
- Ubiquitin and proteasome pathways
- Sepsis Diagnosis and Treatment
Augusta University Health
2017-2024
Meso Scale Discovery (United States)
2024
University of Florida
2017-2020
University of Florida Health
2017-2020
Florida College
2017-2020
University of Florida Health Science Center
2017-2020
Frederick National Laboratory for Cancer Research
2010-2019
National Cancer Institute
2010-2019
Center for Cancer Research
2010-2019
Austin College
2019
Carbohydrates participate in almost every aspect of biology from protein sorting to modulating cell differentiation and cell–cell interactions. To date, the majority data gathered on glycan expression has been obtained via analysis with either anti-glycan antibodies or lectins. A detailed understanding specificities these reagents is critical carbohydrates biological systems. Glycan microarrays are increasingly used determine binding specificity glycan-binding proteins (GBPs). In this study,...
Chemical biology studies, exemplified by metabolic glycoengineering experiments that employ short chain fatty acid (SCFA)-hexosamine monosaccharide hybrid molecules, often suffer from off-target effects. Here we demonstrate systematic structure–activity relationship (SAR) studies can deconvolute multiple biological activities of SCFA-hexosamine analogues demonstrating triacylated monosaccharides, including both n-butyrate- and acetate-modified ManNAc analogues, had dramatically different...
Anti-glycan antibodies are an abundant subpopulation of serum with critical functions in many immune processes. Changes the levels these can occur onset disease, exposure to pathogens, or vaccination. As a result, there has been significant interest exploiting anti-glycan as biomarkers for diseases. Serum contains mixture that recognize same antigen, and competition binding potentially influence detection antibody subpopulations more relevant disease The most isotypes IgG, IgM, IgA, but...
This study investigates the breadth of cellular responses engendered by short chain fatty acid (SCFA)−hexosamine hybrid molecules, a class compounds long used in "metabolic glycoengineering" that are now emerging as drug candidates. First, "mix and match" strategy showed different SCFA (n-butyrate acetate) appended to same core sugar altered biological activity, complementing previous results [Campbell et al. J. Med. Chem. 2008, 51, 8135−8147] where single type elicited distinct responses....
Glycosylation is an important post-translational modification that influences many biological processes critical for development, normal physiologic function, and diseases. Unfortunately, progress toward understanding the roles of glycans in biology has been slow due to challenges studying proteins interact with them. Glycan microarrays provide a high-throughput approach rapid analysis carbohydrate-macromolecule interactions. Protocols detailed here are intended help laboratories basic...
Glycan density and linker composition significantly influence binding on glycan microarrays.
We retrospectively compared anticoagulation with heparin and bivalirudin for 32 consecutive children under 18 years old during extracorporeal membrane oxygenation (ECMO) in our pediatric cardiac intensive care unit (PCICU). Between September 2015 January 2018, 14 patients received heparin, 13 venoarterial (VA), 1 venovenous (VV). From February 2018 to 2019, (all VA). The mean (standard deviation [SD]) percentage of time therapeutic activated partial thromboplastin clotting was 54 (14%) 57...
Per-butanoylated N-acetyl-d-mannosamine (Bu4ManNAc), a SCFA-hexosamine cancer drug candidate with activity manifest through intact n-butyrate-carbohydrate linkages, reduced the invasion of metastatic MDA-MB-231 breast cells unlike per-butanoylated-d-mannose (Bu5Man), clinically tested compound that did not alter cell mobility. To gain molecular-level insight, therapeutic targets implicated in metastasis were investigated. The active Bu4ManNAc both MUC1 expression and MMP-9 (via...
Carbohydrate-binding antibodies play a critical role in basic and clinical research. Monoclonal that bind glycans are used to measure carbohydrate expression, serum can be important elements of the immune response pathogens vaccines. Carbohydrate antigen arrays, or glycan have emerged as powerful tools for high-throughput analysis carbohydrate-protein interactions. Our group has focused on development application neoglycoprotein unique array format wherein carbohydrates covalently attached...
Background: Although heparin has previously been the anticoagulant of choice during mechanical circulatory support (MCS), there is a lack consistency in dose-response pediatric patients. Bivalirudin offers more consistent adults; however, are limited data for pediatrics use. Objective: The purpose was to characterize usage, dosage, and safety profile bivalirudin when used MCS tertiary care hospital. Methods: A retrospective review patients receiving extracorporeal membrane...
BACKGROUND: Blood loss from diagnostic procedures in critically ill patients needs to be minimized. Traditionally, when drawing blood arterial lines, the initial sample used clear line has been discarded (open method). Use of a temporary reservoir enables this discard returned patient (closed METHODS: Critically surgical were prospectively randomized open or closed method lines. sampling was measured both groups. RESULTS: A comparison study (n = 1657) these two methods revealed that...
This review describes short-chain fatty acid (SCFA)-sugar hybrids, exemplified by the lead compound But 4 ManNAc where n-butyrate is linked to N-acetyl-D-mannosamine (ManNAc) in a single molecule, that merge two emerging modes of cancer therapy First, histone deacetylase (HDAC) inhibitor remodels chromatin, thereby influencing patterns gene expression 'make bad cells go good'. Second, dedicated precursor for sialic biosynthesis, sugar gaining increasing recognition as an important modulator...
Background: Risperidone dosing and safety data are limited in patients ≤2 years of age. Objective: To describe the strategies, safety, tolerability risperidone infants Methods: An institutional review board-approved retrospective study was conducted a 24-bed pediatric intensive care unit at an academic medical center age receiving for management ICU delirium. The primary outcome mean initial daily dose risperidone. Secondary outcomes included dose, frequency, treatment duration, adverse...
Background: The HIV-1 envelope is covered with glycans that provide structural integrity and protect conserved regions from host antibody responses. However, these are often the target of broadly neutralizing antibodies (bNAbs) emerge in some HIV-infected individuals. We aimed to determine whether antiglycan IgG a general response infection or specific individuals who develop bNAbs. Methods: binding was assessed using arrays contained 245 unique components including N-linked carbohydrates,...
We sought to determine if higher plasma levels of brain injury biomarkers neurofilament light (NfL), phosphorylated tau 181 (pT181), tau, and ubiquitin C-terminal hydrolase L1 (UCHL1) were associated with unfavorable outcomes in children supported on extracorporeal membrane oxygenation (ECMO) without preceding cardiac arrest. conducted a secondary analysis two-center prospective observational study ECMO patients 0-<18 years. Plasma concentrations NfL, pT181, UCHL1 measured days 1, 2 3....
Recent progress toward an HIV vaccine highlights both the potential of vaccines to end AIDS pandemic and need boost efficacy by incorporating additional strategies. Although many aspects immune response can contribute efficacy, key factors have not been defined fully yet. A particular area that may yield new insights is anti-glycan responses, such as those against glycan shield uses evade system. In this study, we used microarray technology evaluate antibody responses induced SIV vaccination...