A5036746523- Metalloenzymes and iron-sulfur proteins
- Per- and polyfluoroalkyl substances research
- Electrocatalysts for Energy Conversion
- Hydrogen Storage and Materials
- Carcinogens and Genotoxicity Assessment
- Microbial Fuel Cells and Bioremediation
- Toxic Organic Pollutants Impact
- Edible Oils Quality and Analysis
- Birth, Development, and Health
- Effects and risks of endocrine disrupting chemicals
- Pharmacogenetics and Drug Metabolism
- Ammonia Synthesis and Nitrogen Reduction
- Cassava research and cyanide
- Genomics, phytochemicals, and oxidative stress
- Microbial metabolism and enzyme function
- Potato Plant Research
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
- Anaerobic Digestion and Biogas Production
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Photosynthetic Processes and Mechanisms
- Adipose Tissue and Metabolism
- Estrogen and related hormone effects
- Cell Adhesion Molecules Research
Federal Institute for Risk Assessment
2015-2025
University of Veterinary Medicine Hannover, Foundation
2014
Universitätsklinikum des Saarlandes
2014
Saarland University
2014
Federal Department of Home Affairs
2013
Humboldt-Universität zu Berlin
1999-2010
Technische Universität Berlin
2003
Max Planck Society
2003
Freie Universität Berlin
2003
GTx (United States)
1998
The regulatory H2-sensing [NiFe] hydrogenase of the β-proteobacterium Ralstonia eutropha displays an Ni−C "active" state after reduction with H2 that is very similar to reduced standard hydrogenases. Pulse electron nuclear double resonance (ENDOR) and four-pulse ESEEM (hyperfine sublevel correlation, HYSCORE) spectroscopy are applied obtain structural information on this via detection electron−nuclear hyperfine coupling constants. Two proton couplings determined by analysis ENDOR spectra...
Hydrogenases, abundant proteins in the microbial world, catalyze cleavage of H2 into protons and electrons or evolution by proton reduction. Hydrogen metabolism predominantly occurs anoxic environments mediated hydrogenases, which are sensitive to inhibition oxygen. Those microorganisms, thrive oxic habitats, contain hydrogenases that operate presence We have selected H2-sensing regulatory [NiFe] hydrogenase Ralstonia eutropha H16 investigate molecular background its oxygen tolerance....
Drug-induced liver injury (DILI) cannot be accurately predicted by animal models. In addition, currently available in vitro methods do not allow for the estimation of hepatotoxic doses or determination an acceptable daily intake (ADI). To overcome this limitation, vitro/in silico method was established that predicts risk human DILI relation to oral and blood concentrations. This can used estimate if maximal concentration (Cmax) test compound is known. Moreover, ADI estimated even compounds...
The acute toxicity of cyanide is determined by its peak levels reached in the body. Compared to ingestion free cyanide, lower may be expected after consumption foods containing cyanogenic glycosides with same equivalent dose cyanide. This due possible delayed and/or incomplete release from depending on many factors. Data bioavailability high as presented herein were necessary allow a meaningful risk assessment for these foods. A crossover study was carried out 12 healthy adults who consumed...
Abstract Perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic (PFOS) are man-made chemicals that used for the fabrication of many products with water- dirt-repellent properties. The toxicological potential both substances is currently under debate. In a recent Scientific Opinion, European Food Safety Authority (EFSA) has identified increased serum total cholesterol levels in humans as one major critical effect being associated exposure to PFOA or PFOS. animal studies, induced decrease...
ABSTRACT Two energy-generating hydrogenases enable the aerobic hydrogen bacterium Ralstonia eutropha (formerly Alcaligenes eutrophus ) to use molecular as sole energy source. The complex synthesis of nickel-iron-containing enzymes has be efficiently regulated in response H 2 , which is available low amounts environments. sensing R. achieved by a hydrogenase-like protein controls hydrogenase gene expression concert with two-component regulatory system. In this study we show that sensor...
Per- and polyfluoroalkyl substances (PFAS) are synthetic chemicals used in various industrial consumer products. They have gained attention due to their ubiquitous occurrence the environment potential for adverse effects on human health, often linked immune suppression, hepatotoxicity, altered cholesterol metabolism. This study aimed explore impact of ten individual PFAS, 3H-perfluoro-3-[(3-methoxypropoxy) propanoic acid] (PMPP/Adona), ammonium perfluoro-(2-methyl-3-oxahexanoate)...
Previous genetic studies have revealed a multicomponent signal transduction chain, consisting of an H2 sensor, histidine protein kinase, and response regulator, which controls hydrogenase gene transcription in the proteobacterium Ralstonia eutropha. In this study, we isolated sensor demonstrated that purified forms complex with kinase. Biochemical spectroscopic analysis is cytoplasmic [NiFe]-hydrogenase unique features. The H2-oxidizing activity was 2 orders magnitude lower than standard...
Abstract Per- and polyfluoroalkyl substances (PFAS) are environmental contaminants with various adverse health effects in humans including disruption of lipid metabolism. Aim the present study was to elucidate molecular mechanisms PFAS-mediated on metabolism human cells. Here, we examined impact a number PFAS (PFOS, PFOA, PFNA, PFDA, PFHxA, PFBA, PFHxS, PFBS, HFPO-DA, PMPP) some exposure-relevant mixtures being composed PFOS, PFNA PFHxS HepaRG cells, an vitro model for hepatocytes. At near...
The hypX gene of the facultative lithoautotrophic bacterium Ralstonia eutropha is part a cassette accessory genes (the hyp cluster) required for proper assembly active site [NiFe]-hydrogenases in bacterium. A deletion led to severe growth retardation under conditions with 5 or 15% oxygen, when was dependent on activity soluble NAD+ -reducing hydrogenase. enzymatic and infrared spectral properties hydrogenase purified from HypX-negative strain were compared those an enzyme HypX-positive...