A5029867190- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Phagocytosis and Immune Regulation
- Reproductive Biology and Fertility
- Chronic Myeloid Leukemia Treatments
- Tuberculosis Research and Epidemiology
- Endometriosis Research and Treatment
- Cytokine Signaling Pathways and Interactions
- Reproductive System and Pregnancy
- Birth, Development, and Health
- Sperm and Testicular Function
- Dermatology and Skin Diseases
- Immune Response and Inflammation
- Ovarian cancer diagnosis and treatment
- IL-33, ST2, and ILC Pathways
- Pregnancy and preeclampsia studies
- Mitochondrial Function and Pathology
- Ovarian function and disorders
- Metabolism and Genetic Disorders
- Reproductive Physiology in Livestock
- Gestational Trophoblastic Disease Studies
- TGF-β signaling in diseases
- Sexual Differentiation and Disorders
University of Miyazaki
2016-2024
Japan Agency for Medical Research and Development
2016-2024
Gakushuin University
2024
Keio University
2011-2014
Japan Science and Technology Agency
2011-2014
Kōchi University
2003-2014
RIKEN Center for Integrative Medical Sciences
2007-2012
Kochi Medical School Hospital
2001-2002
Tohoku University
1986-1998
Matsunami General Hospital
1996
In order to study alterations of angiogenesis and blood vessel regression through ovarian cycle in human ovaries we quantitatively examined vascularity various stages 24 normal ovaries. Vascular density (VD; numbers/10(-7) m2) endothelial area each (EA; 10(-12) m2/vessel) were evaluated using immunohistochemistry CD34 CAS 200 image analysis system. Small-sized vessels sporadically observed stroma adjacent primordial or primary follicles (6.73 +/- 1.83 for VD 113.58 21.80 EA). Formation...
Abstract IL-9 is a pleiotropic cytokine that can regulate autoimmune and allergic responses. Th9 cells develop from naive T or Th2 through stimulation by TGF-β in vitro. In this study, we demonstrated Smad2 Smad3 are necessary for production an OVA-induced asthma model using cell–specific Smad2- Smad3-deficient mice. were also redundantly essential signaling to induce histone modifications Il9 transcription. Although Smad2/3 was recruited the promoter stimulation, they not sufficient...
Abstract Endosomal toll-like receptor (TLR)-mediated detection of viral nucleic acids (NAs) and production type I interferon (IFN-I) are key elements antiviral defense, while inappropriate recognition self NAs with the induction IFN-I responses is linked to autoimmunity such as psoriasis systemic lupus erythematosus. Plasmacytoid dendritic cells (pDCs) specialized in robust secretion by engagement endosomal TLRs predominantly express sialic acid-binding Ig-like lectin (Siglec)-H. However,...
Abstract Dendritic cells (DCs) comprise several subsets that are critically involved in the initiation and regulation of immunity. Clec4A4/DC immunoreceptor 2 (DCIR2) is a C-type lectin receptor (CLR) exclusively expressed on CD8α − conventional DCs (cDCs). However, how Clec4A4 controls immune responses through function cDCs remains unclear. Here we show regulatory for activation impairs inflammation T-cell Clec4a4 −/− enhanced cytokine production priming following Toll-like (TLR)-mediated...
Dendritic cells (DCs) are composed of multiple subsets that play a dual role in inducing immunity and tolerance. However, it is unclear how CD205 + conventional DCs (cDCs) control immune responses vivo. Here we generated knock-in mice with the selective conditional ablation cDCs. cDCs contributed to antigen-specific priming CD4 T under steady-state conditions, whereas they were dispensable for T-cell inflammatory conditions. In contrast, required CD8 generate cytotoxic lymphocytes (CTLs)...
Mitochondria play a central role in the function of brown adipocytes (BAs). Although mitochondrial biogenesis, which is indispensable for thermogenesis, regulated by coordination between nuclear DNA transcription and transcription, molecular mechanisms development during BA differentiation are largely unknown. Here, we show importance ER-resident sensor PKR-like ER kinase (PERK) thermogenesis adipose tissue. During differentiation, PERK physiologically phosphorylated independently stress....
Ad4-binding protein (Ad4BP) has been demonstrated recently as a transcription factor that serves general regulator of all steroidogenic P450 genes. We examined the expression Ad4BP in 32 normal cycling human ovaries and 22 ovarian sex cord stromal tumors by immunoblotting immunohistochemistry. Immunoblotting revealed single band 53 kilodaltons, corresponding to mol wt Ad4BP. also correlated with immunolocalization enzymes (side-chain cleavage cytochrome P450, 17 alpha-hydroxylase,...
Disruption of intestinal homeostasis can lead to inflammatory bowel diseases endowed susceptibility genes and environmental factors affecting accumulation activation colitogenic phagocytes. Plasmacytoid dendritic cells (pDCs) are immune that had been proposed control innate adaptive immunity through the massive secretion type I interferon (IFN-I). However, contribution pDCs progression inflammation remains unclear. Here we show a critical role in initiation acute colonic using...
While dysbiosis in the gut is implicated impaired induction of oral tolerance generated mesenteric lymph nodes (MesLNs), how affects this process remains unclear. Here, we describe that antibiotic-driven causes dysfunction CD11c+CD103+ conventional dendritic cells (cDCs) MesLNs, preventing establishment tolerance. Deficiency cDCs abrogates generation regulatory T MesLNs to establish Antibiotic treatment triggers intestinal linked colony-stimulating factor 2 (Csf2)-producing group 3 innate...
We evaluated the immunolocalization of steroidogeriic enzymes involved in production ovarian steroids, including cholesterol side-chain cleavage enzyme (P450scc), 3α-hydroxysteroid dehydrogenase (HSD), 17α-hydroxylase (P450c17) and aromatase (P450arom), oestrogen receptor (ER) androgen (AR), a steroidogenic transcription factor, Ad4-binding protein (Ad4BP) cell cycle-related nuclear antigen, Ki67, five patients with polycystic syndrome (PCOS). Results were compared those from normal cycling...
We evaluated the immunolocalization and characterization of macrophages in 28 normal cycling human ovaries. Two primary antibodies were used to detect macrophages: PGM1, a general marker for macrophages, 25F9 which is specific phagocytosing macrophages. Spindle-shaped cells positive PGM1 but negative observed stroma (123.6 +/- 1.05 cells/10(-6) m2) theca layer follicle (mean ranged from 22.61 53.79) number these did not change throughout cycle. After ovulation, with ballooning bodies began...
We investigated, using an image analysis system, the immunohistochemical localization of leukocyte subpopulations and human antigen (HLA)-DR in 30 normal-cycling ovaries order to better understand local immunological events ovaries. All subtypes T lymphocytes examined (CD3+, CD4+ CD8+ cells) were sporadically observed stroma theca layers follicles throughout menstrual cycle (ranging from 4.32 6.25 cells/10(-7) m2, 1.67 3.33 m2 2.33 3.44 respectively for three subtypes), subsequently,...
OBJECTIVE--To define the concentrations of inhibin in serum and tissue patients with hydatidiform mole assess their value as a clinical marker condition. DESIGN--Prospective study new mole, comparison paired observations, case-control analysis. SETTING--A university hospital, two large public hospitals, private women9s clinic Japan. PATIENTS--Seven consecutive referred seen over four months newly diagnosed complete including one whom was accompanied by viable twin fetuses (case excluded from...
Suppression of IL-2 βproduction from T cells is an important process for the immune regulation by TGF-β. However, mechanism which this suppression occurs remains to be established. Here, we demonstrate that Smad2 and Smad3, two major TGF-β-downstream transcription factors, are redundantly essential TGF-β-mediated production in CD4+ using Smad2- Smad3-deficient cells. Both Smad3 were recruited into proximal region promoter response We then investigated histone methylation status promoter....