A5026978926- Gut microbiota and health
- Food composition and properties
- Cytokine Signaling Pathways and Interactions
- Probiotics and Fermented Foods
- Immune Cell Function and Interaction
- NF-κB Signaling Pathways
- Psoriasis: Treatment and Pathogenesis
- T-cell and B-cell Immunology
- Microbial Metabolites in Food Biotechnology
- Gastrointestinal motility and disorders
- Monoclonal and Polyclonal Antibodies Research
- Chemokine receptors and signaling
- Immunodeficiency and Autoimmune Disorders
- Kruppel-like factors research
- Bioactive Compounds and Antitumor Agents
- Cancer Mechanisms and Therapy
- Urticaria and Related Conditions
- Dermatology and Skin Diseases
- Cancer Research and Treatments
- Cancer-related gene regulation
- Fibroblast Growth Factor Research
- Nutrition and Health in Aging
- Freezing and Crystallization Processes
- Mast cells and histamine
- Pharmacological Effects of Natural Compounds
Teijin (Japan)
2019-2020
Keio University
2011-2013
Japan Science and Technology Agency
2011
Kurume University
2011
Regulatory T cells (Treg cells) maintain immune homeostasis by limiting inflammatory responses. SOCS1 (suppressor of cytokine signaling 1), a negative regulator signaling, is necessary for the suppressor functions Treg in vivo, yet detailed mechanisms remain to be clarified. We found that Socs1−/− produced high levels IFN-γ and rapidly lost Foxp3 when transferred into Rag2−/− mice or cultured vitro, even though CNS2 (conserved noncoding DNA sequence 2) enhancer region was fully demethylated....
Abstract IL-9 is a pleiotropic cytokine that can regulate autoimmune and allergic responses. Th9 cells develop from naive T or Th2 through stimulation by TGF-β in vitro. In this study, we demonstrated Smad2 Smad3 are necessary for production an OVA-induced asthma model using cell–specific Smad2- Smad3-deficient mice. were also redundantly essential signaling to induce histone modifications Il9 transcription. Although Smad2/3 was recruited the promoter stimulation, they not sufficient...
The protein known as Spred1 (Sprouty-related Ena/VASP homology-1 domain-containing protein) has been identified a negative regulator of growth factor-induced ERK/mitogen-activated kinase activation. also implicated the target microRNA-126 (miR126), miRNA located within Egfl7 gene, and is involved in regulation vessel development through its role regulating VEGF signaling. In this study, we examined miR126 hematopoietic system, shown to be overexpressed leukemic cells. levels were...
Suppression of IL-2 βproduction from T cells is an important process for the immune regulation by TGF-β. However, mechanism which this suppression occurs remains to be established. Here, we demonstrate that Smad2 and Smad3, two major TGF-β-downstream transcription factors, are redundantly essential TGF-β-mediated production in CD4+ using Smad2- Smad3-deficient cells. Both Smad3 were recruited into proximal region promoter response We then investigated histone methylation status promoter....
Butyric acid produced in the intestine by butyric acid-producing bacteria (BAPB) is known to suppress excessive inflammatory response and may prevent chronic disease development. We evaluated whether fiber-rich barley intake increases BAPB gut concomitantly feces. Eighteen healthy adults received granola containing functional (BARLEYmax®) once daily for four weeks. Fecal DNA before intake, after one month was analyzed using 16S rRNA gene sequencing assess microbial diversity, composition at...
Atopic dermatitis (AD) is a common pruritic inflammatory disease triggered by defective skin barrier and immunodysregulation. AD has been considered typical example of Th2 response associated with allergic disease. In the early phases disease, symptoms include IgE hyperproduction, eosinophil accumulation, mast cell activation; in chronic phase, Th1-dominant immune also observed at sites lesions. The role IL-17-producing Th (Th17) cells not established. current study, we found that pyridone 6...
We investigated whether supplementation with the barley line BARLEYmax (Tantangara; BM), which contains three fermentable fibers (fructan, β-glucan, and resistant starch), modifies microbiota in cecal distal colonic digesta addition to short-chain fatty acids (SCFAs) production more favorably than a high-β-glucan (BG012; BG). Male Sprague–Dawley rats were randomly divided into 3 groups that fed an AIN-93G-based diet contained 5% fiber provided by cellulose (control), BM or BG. Four weeks...
Inulin-type fructans are known to exert different effects on the fermentation profile depending average and range of degree polymerization (DP). Here, swine fecal cultures were used investigate prebiotic properties native chicory inulin (NIN), extracted from root, synthetic (SIN), which has a narrower DP distribution than NIN. Both NIN SIN showed effects, but exhibited significant decrease in pH increase production propionate butyrate compared SIN. There also differences succinate lactate,...
e15104 Background: Base editing without DNA cleaving is a technique that introduces point mutations using enzymes induce base conversions. Generally, only the mutation occurs, achieving high level of precision. can easily be applied to bacteria, for which often lethal. With Bio Palette's core technology editing, Target-AID, we quickly achieve required therapeutic effects by making pinpoint modifications bacteria. Immune checkpoint inhibitors (ICIs) targeting CTLA-4, PD-1, or PD-L1 have shown...
大麦品種BARLEYmax (BM) は一般品種の大麦より食物繊維やレジスタントスターチを豊富に含む。本研究では一般大麦品種であるハインドマーシュならびに対照区であるセルロースと比較してBMのin vitroにおける腸内発酵特性を検討した。実験1では2種の大麦を同等に使用し, 実験2ではBMに難消化性画分が多く含まれることを考慮して検討した。大麦試料を消化酵素により加水分解してその残渣物をin vitro培養槽に供試し, 48時間の培養試験を行った。実験1において, BM添加区はハインドマーシュ添加区より培養後期での高い短鎖脂肪酸産生を示した。実験2ではそれに加えて, BM添加区は培養期間を通して短鎖脂肪酸産生の増加およびアンモニア態窒素の低下を示した。以上の結果から, BMは短鎖脂肪酸を持続的に産生し, さらにその多量な難消化性成分により, 有効な腸内発酵特性を示す可能性が示唆された。