A5045700494- Angiogenesis and VEGF in Cancer
- Lymphatic System and Diseases
- Chemokine receptors and signaling
- Interprofessional Education and Collaboration
- Immunotherapy and Immune Responses
- Global Health Workforce Issues
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Sympathectomy and Hyperhidrosis Treatments
- Hippo pathway signaling and YAP/TAZ
- Cancer Cells and Metastasis
- Innovations in Medical Education
- Education and Technology Integration
- Service-Learning and Community Engagement
- Kruppel-like factors research
- Hepatocellular Carcinoma Treatment and Prognosis
- Respiratory Support and Mechanisms
- Tissue Engineering and Regenerative Medicine
- Vascular Tumors and Angiosarcomas
- Axon Guidance and Neuronal Signaling
- Abdominal Trauma and Injuries
- Mast cells and histamine
- Ubiquitin and proteasome pathways
- Liver Diseases and Immunity
- Peptidase Inhibition and Analysis
Indiana University
2025
University School
2025
Indiana University School of Medicine
2025
Moffitt Cancer Center
2025
University of North Dakota
2018-2022
Ludwig Cancer Research
2011-2019
The Royal Melbourne Hospital
2007-2019
The University of Melbourne
2011-2019
Peter MacCallum Cancer Centre
2011-2019
O'Brien Institute
2015-2019
The VEGFC/VEGFR3 signaling pathway is essential for lymphangiogenesis (the formation of lymphatic vessels from pre-existing vasculature) during embryonic development, tissue regeneration and tumor progression. recently identified secreted protein CCBE1 indispensible development. role orthologs highly conserved in zebrafish, mice humans with mutations causing generalized dysplasia lymphedema (Hennekam syndrome). To date, the mechanism by which acts remains unknown. Here, we find that ccbe1...
Vascular endothelial growth factor (VEGF)-D is a secreted glycoprotein that induces angiogenesis and lymphangiogenesis. It consists of central domain, containing binding sites for VEGF receptor-2 (VEGFR-2) VEGFR-3, N- C-terminal propeptides. from the cell as homodimers full-length form can be proteolytically processed to remove was recently shown, using adenoviral gene delivery, fully VEGF-D in vivo, whereas does not. To better understand these observations, we monitored effect processing on...
Metastasis via the lymphatic vasculature is an important step in cancer progression. The formation of new vessels (lymphangiogenesis), or remodeling existing lymphatics, thought to facilitate entry and transport tumor cells into on distant organs. migration endothelial (LEC) toward guidance cues critical for lymphangiogenesis. While chemokines are known provide directional navigation migrating immune cells, their role mediating LEC during tumor-associated lymphangiogenesis not well defined....
The signaling proteins and pathways involved in lymphatic endothelial cell migration are identified through a functional screen.
Cardiac resident stem cells (CRSCs) hold much promise to treat heart disease but this remains a controversial field. Here, we describe novel population of CRSCs, which are positive for W8B2 antigen and were obtained from adult human atrial appendages. W8B2(+) CRSCs exhibit spindle-shaped morphology, clonogenic capable self-renewal. show high expression mesenchymal not hematopoietic nor endothelial markers. expressed GATA4, HAND2, TBX5, C-KIT, SCA-1, NKX2.5, PDGFRα, ISL1, or WT1. can...
Mast cells (MCs), a type of granulocytic immune cell, can be both pro- and anti-tumorigenic in colorectal cancer (CRC). We hypothesized that these contrasting findings may part due to di_erential interactions MCs with CRC subtypes. BRAF mutant uniquely contains intestinal secretory cell types. In this study, we demonstrated are enriched CRC, likely because they recruited by factors released from cells. To investigate the functional consequences MC-CRC interactions, performed direct coculture...
Breast cancer remains the most frequently diagnosed and second highest cause of death in females. Metastatic recurrence that is resistant to traditional therapies presents a major challenge, necessitating development an innovative treatment strategy. Immunotherapy has gained popularity cancer, particularly melanoma, lung more recently breast cancer. Major developments immunotherapy have been made with better understanding tumor microenvironment how can be manipulated induce anti-tumor immune...
VEGF-D is a mitogen for endothelial cells that promotes tumor growth and metastatic spread in animal models, expression of which correlates with lymph node metastasis some human cancers. It secreted from the cell as full-length form propeptides flanking central region containing binding sites VEGFR-2 VEGFR-3, receptors signal angiogenesis lymphangiogenesis. The can be cleaved VEGF-D, enhancing affinity VEGFR-3 vitro; however, importance this processing cancer unclear. To explore necessity...
Leakage of fluid from blood vessels, leading to oedema, is a key feature many diseases including hyperoxic acute lung injury (HALI), which can occur when patients are ventilated with high concentrations oxygen (hyperoxia). The molecular mechanisms driving vascular leak and oedema in HALI poorly understood. VEGF-D protein that promotes vessel overexpressed tissues, but the role endogenous pathological was unknown. To address these issues, we exposed Vegfd-deficient mice hyperoxia. resulting...
The benefits of adult stem cells for repair the heart have been attributed to repertoire salutary paracrine activities they appear exert. We previously isolated human W8B2+ cardiac (CSCs) and found powerfully influence cardiomyocytes endothelial collectively promote regeneration. Here, complexity CSC secretomes was characterised examined in more detail. Using ion exchange chromatography separate soluble proteins based on their net surface charge, secreted factors responsible pro-survival...
The accuracy of Acoustic Radiation Force Impulse (ARFI) imaging has been validated in the setting hepatitis C, however, fatty liver disease (FLD) less well-established. aim this study was to assess ARFI hepatic steatosis.Patients with biopsy proven or sonographically diagnosed steatosis were assessed for trends including: inter-operator concordance, interquartile range, failure rate, relationship between velocity and severity, concordance fibrosis scores.Three hundred forty-nine patients (53...
The purpose of this study was to examine students’ perceptions and performance outcomes a virtual fieldwork using Simucase® in conjunction with supplemental activities debriefing opportunities. simulation replaced Level I clinic-based experiences that were cancelled due COVID-19. Quantitative qualitative data collected modified evaluation tool assess student for students the Year one Occupational Therapy Doctorate (OTD) cohort (n=57) two Master (MOT) enrolled public university rural Midwest....
Targeted ultrasound of the liver (TUSL) has been proposed as a new approach in chronic disease to meet increasing demands on services this patient population. This study analyses impact TUSL examination time.Retrospective cohort analysis time taken perform consecutive patients pre- (n = 230) and post- 147) introduction TUSL. Within each cohort, were subdivided into three categories based clinical indication: Group 1. hepatocellular carcinoma (HCC) surveillance; 2. detection cirrhosis,...
An online survey was conducted of 225 occupational therapy (OT) practitioners living in the rural states North Dakota and Wyoming to explore practice patterns influence college experience on employment choice. Findings showed that had greater variability hours spent working (5.5 more per week, p=.028), one work location average (p=.006). Therapists urban settings 15% time fieldwork education than their counterparts (p=.021). Rural choice influenced by participation Level I II (p=.002) but...
<div>Abstract<p>Metastasis via the lymphatic vasculature is an important step in cancer progression. The formation of new vessels (lymphangiogenesis), or remodeling existing lymphatics, thought to facilitate entry and transport tumor cells into on distant organs. migration endothelial (LEC) toward guidance cues critical for lymphangiogenesis. While chemokines are known provide directional navigation migrating immune cells, their role mediating LEC during tumor-associated...
<p>Supplementary Figures S1-S8 S1 Heat map of chemokines and receptors S2 Immunohistochemical staining lymphatic vessels S3 Expression VEGF-D in cancer cell lines S4 genes melanoma patient samples S5 Chemokine secretion by tumour cells S6 induced migration LEC S7 Chemokines attract vivo S8 CCL-293EBNA growth CCL27 secretion</p>
<p>Supplementary Figures S1-S8 S1 Heat map of chemokines and receptors S2 Immunohistochemical staining lymphatic vessels S3 Expression VEGF-D in cancer cell lines S4 genes melanoma patient samples S5 Chemokine secretion by tumour cells S6 induced migration LEC S7 Chemokines attract vivo S8 CCL-293EBNA growth CCL27 secretion</p>
<div>Abstract<p>Metastasis via the lymphatic vasculature is an important step in cancer progression. The formation of new vessels (lymphangiogenesis), or remodeling existing lymphatics, thought to facilitate entry and transport tumor cells into on distant organs. migration endothelial (LEC) toward guidance cues critical for lymphangiogenesis. While chemokines are known provide directional navigation migrating immune cells, their role mediating LEC during tumor-associated...