A5101865281- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Circular RNAs in diseases
- Bacterial Identification and Susceptibility Testing
- NF-κB Signaling Pathways
- Acute Kidney Injury Research
- Infective Endocarditis Diagnosis and Management
- Cancer, Hypoxia, and Metabolism
- Advanced Glycation End Products research
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Sepsis Diagnosis and Treatment
- Heart Failure Treatment and Management
- Corneal Surgery and Treatments
- Traditional Chinese Medicine Analysis
- Respiratory Support and Mechanisms
- Kruppel-like factors research
- Vibrio bacteria research studies
- Clostridium difficile and Clostridium perfringens research
- interferon and immune responses
- S100 Proteins and Annexins
- Hydrogen's biological and therapeutic effects
Hangzhou Medical College
2017-2025
Zhejiang Provincial People's Hospital
2014-2025
Zhejiang Hospital
2013
Metagenomic next-generation sequencing (mNGS) and droplet digital PCR (ddPCR) have recently demonstrated a great potential for pathogen detection. However, few studies been undertaken to compare these two nucleic acid detection methods identifying pathogens in patients with bloodstream infections (BSIs). This prospective study was thus conducted diagnostic applications clinical setting critically ill suspected BSIs. Upon suspicion of BSIs, whole blood samples were simultaneously drawn ddPCR...
BackgroundAn altered microRNA profile exists in many infectious diseases, including sepsis. CD39+ regulatory T-cells (Tregs) have a remarkable immunosuppressive effect and play an important role the regulation of immune balance However, correlation between changes ratio Tregs sepsis patients has not yet been reported. The expression was analyzed this study. Moreover, microRNAs disease severity prognosis investigated. Furthermore, percentage peripheral blood investigated further verified...
The relatively long turnaround time and low sensitivity of traditional blood culture-based diagnosis may delay effective antibiotic therapy for patients with bloodstream infections (BSIs). A rapid sensitive pathogen detection method is urgently required to reduce the morbidity mortality associated BSIs. Acinetobacter baumannii Klebsiella pneumoniae are two major microorganisms that cause Here we report a novel droplet digital polymerase chain reaction (ddPCR) assay can detect A. K. in...
lncRNAs play important roles in lipopolysaccharide- (LPS-) induced acute lung injury. But the mechanism still needs further research. In present study, we investigate functional role of lncRNA-SNHG14/miR-223-3p/Foxo3a pathway LPS-induced ALI and tried to confirm its regulatory effect on autophagy. Transcriptomic profile changes were identified by RNA-seq LPS-treated alveolar type II epithelial cells. The expression confirmed using qRT-PCR west blot. binding relationship...
Bloodstream infection (BSI) is a life-threatening condition in critically ill patients, but pathogen quantification techniques during treatment are laborious. This study aimed to explore the impact of monitoring DNA load changes and polymicrobial blood by droplet digital polymerase chain reaction (ddPCR) on prognosis patients with BSIs. prospective case series was conducted general intensive care unit Zhejiang Provincial People's Hospital included BSIs from May 2020 January 2021. Pathogens...
// Yibin Wang 1, * , Haijun Huang 2, Renhua Sun 3 Bingyu Chen 4, 5 Fang Han Qian Li Yin Ni Xi 6 Jingquan Liu Xiaozhou Mou 7, 8 and Yuexing Tu 1 Department of Cardiology, Chunan First People's Hospital, Hangzhou 311700, China 2 Infectious Diseases, Zhejiang Provincial Hospital Medical College, 310014, ICU Department, 4 Centre Laboratory Medicine, Transfusion 7 Clinical Research Institute, Medicine Key Tumor Molecular Diagnosis Individualized Province, These authors have contributed equally to...
Tubular damage initiated by inflammatory response and ischemic/hypoxic stress is a hallmark of septic acute kidney injury (AKI), albeit the molecular mechanism coupling two events remains unclear. We investigated intrinsic nature tubular with respect to inflammatory/hypoxic during AKI. Methods: The apoptotic cells LPS stimuli was analyzed before after hypoxia exposure. Cellular ubiquitination, co-immunoprecipitation, GST-pulldown, in vitro protein kinase assay, immunofluorescence CRISPR...
The immunosuppressive, inflammatory microenvironment orchestrated by neutrophil extracellular traps (NETs) plays a principal role in pathogenesis of sepsis. Fibroblast growth factor-inducible molecule 14 (Fn14) has been established as potential target for septic acute kidney injury (AKI), making further therapeutic benefits from combined NETs and Fn14 blockade possible.
Cell apoptosis is one of the main pathological alterations during oxidative stress (OS) injury. Previously, we corroborated that nuclear factor-κB (NF-κB) transactivation confers resistance against OS in mammalian cells, yet underlying mechanisms remain enigmatic. Here report microRNA-19a (miR-19a) transcriptionally regulated by reactive oxygen species (ROS) production and NF-κB deactivation prevents OS-initiated cell through cylindromatosis (CYLD) repression. CYLD contributes to apoptosis,...
Atherosclerosis (AS) is a chronic inflammatory disease characterized by accumulating deposition of lipids in the arterial intima. Notably, macrophages participate centrally pathogenesis this deadly disease. In study, we established AS mouse models order to investigate effect microRNA-133b (miR-133b) on vulnerable plaque formation and vascular remodeling explore potential functional mechanisms. The expression miR-133b was altered or Notch-signaling pathway blocked evaluate proliferation,...
Sepsis-induced acute lung injury is associated with dysregulated inflammatory reactions. MiR-19b-3p level was reported to be downregulated in patients sepsis. To evaluate the role of miR-19b-3p sepsis, cecum ligation and puncture-induced mouse sepsis model lpopolysaccharide (LPS)-treated pulmonary microvascular endothelial cells (PMVECs) were used. For vivo study, tissue harvested for hematoxylin eosin (H&E) staining, tumor necrosis factor-α, interleukin-6 (IL-6), IL-1β, p-p65, p-IκB...
IGF-1 functions as an anti-oxidative stress molecule and some critical patients with sepsis have a lower level of serum IGF-1. However, the association between severity or prognosis remains unclear. This study aimed to elucidate relationship levels sepsis, possible mechanism was analyzed. Clinical characteristics were recorded Serum micro (mi)RNA-1 tested using radioimmunoassay reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, respectively. The A549 cell line...
Tanshinone IIA is a lipophilic abietane diterpene compound, which exhibits protective effects against ischaemia/reperfusion injury; however, the pathways responsible for myocardial activities of tanshinone remain to be elucidated. The aim present study was investigate effect on Janus‑activated kinase (JAK)/signal transducer and activator transcription (STAT) signaling pathway, associated with cardiac dysfunction during ischemia/reperfusion. results demonstrated that protected cells from...
Fibroblast growth factor-inducible molecule 14 (Fn14) plays a principal role in triggering tubular damage during septic acute kidney injury (AKI). Here, we explore the mechanism underlying Fn14 deregulation AKI. We identify as bona fide target of miR-19a, which directly binds to 3<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:msup><mml:mrow/><mml:mrow><mml:mo>′</mml:mo></mml:mrow></mml:msup></mml:math> UTR for repression independent cylindromatosis (CYLD),...
A 27-year-old man sought treatment for fatigue that had lasted 30 h, chest tightness, and shortness of breath 6 accompanied by hypotension. He was admitted to our ED. The patient's BP remained low despite large doses positive inotropic drugs vasoactive drugs. found have an acute course disease, with prodromal symptoms the upper respiratory tract hemodynamic disturbances (BP, 71/52 mm Hg). Bedside ultrasound showed diffuse left ventricular wall motion weakening (ejection fraction, < 30%),...
Background: Acute respiratory distress syndrome (ARDS) a of inflammation with various clinical, radiologic, and physiologic abnormalities. Studies have demonstrated that long non-coding RNAs (lncRNAs) critical roles to play in inflammatory response lung injury. Herein, this study investigated the involvement Kcnq1ot1/miR-381-3p/ETS2 signaling axis lipopolysaccharide (LPS)-induced mouse models ARDS.Results: According microarray analysis, ETS2 was identified as an upregulated gene ARDS....