A5004490979- Acute Kidney Injury Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- NF-κB Signaling Pathways
- Erythropoietin and Anemia Treatment
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Advanced Glycation End Products research
- Cancer, Hypoxia, and Metabolism
- Hippo pathway signaling and YAP/TAZ
- Inflammasome and immune disorders
- Immune Response and Inflammation
- Intensive Care Unit Cognitive Disorders
- Vitamin C and Antioxidants Research
- Chemical Synthesis and Characterization
- Wnt/β-catenin signaling in development and cancer
- Circular RNAs in diseases
- Ubiquitin and proteasome pathways
- Burn Injury Management and Outcomes
- Neurological Complications and Syndromes
- 14-3-3 protein interactions
- Nerve injury and regeneration
- Silicone and Siloxane Chemistry
- Heme Oxygenase-1 and Carbon Monoxide
- S100 Proteins and Annexins
- Organ Transplantation Techniques and Outcomes
Zhejiang Provincial People's Hospital
2015-2025
Hangzhou Medical College
2017-2025
Sun Yat-sen University
2012-2023
The First Affiliated Hospital, Sun Yat-sen University
2013-2023
Zhejiang University
2022
Second Affiliated Hospital of Guangzhou Medical University
2020
Guangzhou Medical University
2020
Beijing University of Chemical Technology
2013
The development of modern biomedical nanotechnology requires conductive polymeric nanofibers with excellent mechanical and biocompatible properties to meet the needs practical applications in complex biological systems. In study, we developed a novel facile method fabricate poly(3,4-ethylenedioxythiophene) (PEDOT) nanofiber mats by electrospinning combined situ interfacial polymerization. PEDOT displayed superior (tensile strength: 8.7 ± 0.4 MPa; Young's modulus: 28.4 3.3 MPa) flexibility,...
Background: This study aimed to assess the relationship between use of dexmedetomidine and incidence acute kidney injury (AKI) in septic shock patients undergoing mechanical ventilation reveal potential mechanism. Methods: Septic were included. Patients randomized into two groups including propofol group group. Plasma samples obtained from veins at 0, 12, 24, 72 120 h after receiving ICU. Results: Cohorts with ICU had similar baseline demographic characteristics. Serum creatinine (SCr) blood...
Abstract Hypoxic microenvironment deregulates metabolic homeostasis in cancer cells albeit the underlying mechanisms involved this process remain hitherto enigmatic. 14-3-3ζ/Yes-associated protein (YAP) axis plays a principal role malignant transformation and tumor development. Here, we report that hypoxia disassembles 14-3-3ζ from YAP thereby promotes nuclear localization mediated by ERK2, which directly binds to D-site of mitogen-activated kinase (MAPK) docking domain Leu98/100...
lncRNAs play important roles in lipopolysaccharide- (LPS-) induced acute lung injury. But the mechanism still needs further research. In present study, we investigate functional role of lncRNA-SNHG14/miR-223-3p/Foxo3a pathway LPS-induced ALI and tried to confirm its regulatory effect on autophagy. Transcriptomic profile changes were identified by RNA-seq LPS-treated alveolar type II epithelial cells. The expression confirmed using qRT-PCR west blot. binding relationship...
Abstract Background Our previous studies demonstrated that eyes absent homolog 4 (EYA4), a member of the eye development‐related EYA family in Drosophila , is frequently methylated and silenced hepatocellular carcinoma (HCC) specimens associated with shorter survival. The current work aimed to explore mechanisms through which EYA4 functions as tumor suppressor HCC. Methods Stable EYA4‐expressing plasmid (pEYA4) transfectants human HCC cell lines Huh‐7 PLC/PRF/5 (PLC) were established....
To investigate the effects of serum amyloid A1 (SAA1) on lipopolysaccharide (LPS) -induced inflammation in vascular smooth muscle cells (VSMCs). SAA1 expression was detected LPS induced VSMCs at different concentrations for time by using Western blotting. After pre-incubation with recombinant protein, were treated 1 μg/ml 24 h. The then divided into Control, siRNA, Nox4 LPS, + siRNA and groups. MTT performed to observe toxicity VSMCs. Lucigenin-enhanced chemiluminescence method used detect...
High-dose ascorbate confers tubular mitophagy responsible for septic acute kidney injury (AKI) amelioration, yet its biological roles in immune regulation remain poorly understood.Methods: The role of macrophage polarization upon high-dose treatment was assessed by fluorescence-activated cell sorter analysis (FACS) vitro and immunofluorescence AKI models LPS-induced endotoxemia (LIE) from Pax8-cre; Atg7 flox/flox mice.The underlying mechanisms were revealed RNA-sequencing, gene set...
Tubular damage initiated by inflammatory response and ischemic/hypoxic stress is a hallmark of septic acute kidney injury (AKI), albeit the molecular mechanism coupling two events remains unclear. We investigated intrinsic nature tubular with respect to inflammatory/hypoxic during AKI. Methods: The apoptotic cells LPS stimuli was analyzed before after hypoxia exposure. Cellular ubiquitination, co-immunoprecipitation, GST-pulldown, in vitro protein kinase assay, immunofluorescence CRISPR...
The immunosuppressive, inflammatory microenvironment orchestrated by neutrophil extracellular traps (NETs) plays a principal role in pathogenesis of sepsis. Fibroblast growth factor-inducible molecule 14 (Fn14) has been established as potential target for septic acute kidney injury (AKI), making further therapeutic benefits from combined NETs and Fn14 blockade possible.
Cell apoptosis is one of the main pathological alterations during oxidative stress (OS) injury. Previously, we corroborated that nuclear factor-κB (NF-κB) transactivation confers resistance against OS in mammalian cells, yet underlying mechanisms remain enigmatic. Here report microRNA-19a (miR-19a) transcriptionally regulated by reactive oxygen species (ROS) production and NF-κB deactivation prevents OS-initiated cell through cylindromatosis (CYLD) repression. CYLD contributes to apoptosis,...
Autophagy and glycolysis are two catabolic processes that manipulate pancreatic ductal adenocarcinoma (PDAC) development in response to hypoxia sensing, yet the underlying mechanism of how they interlinked remain elusive.Methods: The functional roles Unc-51 like kinase 1 2 (ULK1/2) pyruvate M2 (PKM2) transcription under were assessed by chromatin immunoprecipitation, luciferase reporter, glucose consumption lactate production assay.Co-immunoprecipitation, cellular ubiquitination,...
Acute kidney injury (AKI) initiated by sepsis remains a thorny problem despite recent advancements in its clinical management. Having been found to be activated during AKI, fibroblast growth factor-inducible molecule 14 (Fn14) may potential therapeutic target because of involvement the molecular basis injury. Here, we report that LPS induces apoptosis mouse cortical tubule cells mediated Fn14, for which simultaneous Toll-like receptor (TLR)4 activation is required. Mechanistically, TLR4...
Efferocytosis of apoptotic neutrophils (PMNs) by macrophages is helpful for inflammation resolution and injury repair, but the role efferocytosis in intrinsic nature during septic acute kidney (AKI) remains unknown. Here we report that CD47 signal regulatory protein alpha (SIRPα)-the anti-efferocytotic 'don't eat me' signals-are highly expressed peripheral blood mononuclear cells (PBMCs) from patients with AKI samples mice polymicrobial sepsis endotoxin shock. Conditional knockout (CKO)