The development of modern biomedical nanotechnology requires conductive polymeric nanofibers with excellent mechanical and biocompatible properties to meet the needs practical applications in complex biological systems. In study, we developed a novel facile method fabricate poly(3,4-ethylenedioxythiophene) (PEDOT) nanofiber mats by electrospinning combined situ interfacial polymerization. PEDOT displayed superior (tensile strength: 8.7 ± 0.4 MPa; Young's modulus: 28.4 3.3 MPa) flexibility,...

Electrospun nanofiber scaffolds have been shown to accelerate the maturation, improve growth, and direct migration of cells in vitro. Electrospinning is a process which charged polymer jet collected on grounded collector; rapidly rotating collector results aligned nanofibers while stationary collectors result randomly oriented fiber mats. The formed when an applied electrostatic charge overcomes surface tension solution. There minimum concentration for given polymer, termed critical...

Neuritogenesis, neuronal polarity formation, and maturation of axons dendrites are strongly influenced by both biochemical topographical extracellular components. The aim this study was to elucidate the effects polylactic acid electrospun fiber topography on primary motor neuron development, because regeneration is extremely limited in central nervous system could potentially benefit from implementation a synthetic scaffold encourage regrowth. In analysis, we found that aligned randomly...

Three dimensional (3D) cell culture in functional scaffolds to mimic the natural growth state is important for construction of based implants vitro tissue engineering applications. Herein, we report a novel fluffy polypyrrole (PPy) fibrous scaffold (fluffy-PPy scaffold) fabricated by means an improved electrospinning process combined with situ surface polymerization, which PPy hollow fibers are discrete from one another deep interconnected pores ∼100 μm. This unique spatial structure permits...

In this study, a novel three-dimensional fluffy PPy conductive fibrous scaffold (3D-cFSs) was fabricated by electrospinning technique combined with situ surface polymerization. Chemical compositions, morphology were characterized fourier transform infrared (FTIR) and scanning electron microscopy (SEM). The results showed that the average diameter of coated PLLA fibers in 3D-cFSs 2.086 microm, thickness nano-layer -45 nm. These discrete state, size interconnected pores from 50 microm to 100...

Electrospun nanofiber scaffolds have been shown to accelerate the maturation, improve growth, and direct migration of cells in vitro. Electrospinning is a process which charged polymer jet collected on grounded collector; rapidly rotating collector results aligned nanofibers while stationary collectors result randomly oriented fiber mats. The formed when an applied electrostatic charge overcomes surface tension solution. There minimum concentration for given polymer, termed critical...

Advanced scaffold materials are required for liver tissue engineering, to improve primary hepatocyte activity and hepatic function in vitro. The nanotopography of the material plays an important role regulation cell growth function. Therefore, current study, we developed a novel composed type-I collagen coated nanoporous poly(l-lactic acid) (PLLA) fibers (nPFs) provide with combination fibrous porous features culture hepatocytes. interaction between hepatocytes was described by testing...

Nanofibrous scaffolds have been applied widely in tissue engineering to simulate the nanostructure of natural extracellular matrix (ECM) and promote cell bioactivity. The aim this study was design a biocompatible nanofibrous scaffold for blood outgrowth endothelial cells (BOECs) investigate interaction between topography growth. Poly(l-lactic acid) (PLLA) random aligned nanofibers with uniform diameter distribution were fabricated by electrospinning. NH3 plasma etching used create...

In this study, we developed a method to obtain high surface area nanofiber meshes composed of chitosan number molecular weights. This required decreasing the viscosity and tension solution as well optimization electrospinning parameters such applied voltage environmental humidity. These were culture substrate for hepatocytes. The fibers exhibited uniform diameter distribution (average diameter: 112 nm) FTIR results indicate that chemical structure is stable during process. attachment,...

Electrospinning is a technique for producing micro- to nano-scale fibers. Fibers can be electrospun with varying degrees of alignment, from highly aligned completely random. In addition, fibers spun variety materials, including biodegradable polymers such as poly-L-lactic acid (PLLA). These characteristics make suitable scaffolding applications in tissue engineering. Our focus on the use nerve regeneration. We have previously shown that PLLA direct outgrowth both primary sensory and motor...

Despite modern technological advances, the most widely available prostheses provide little functional recovery beyond basic grasping. Although sophisticated upper extremity are available, optimal prosthetic interfaces which give patients high-fidelity control of these artificial limbs limited. We have developed a novel Regenerative Peripheral Nerve Interface (RPNI), consists unit free muscle that has been neurotized by transected peripheral nerve. In conjunction with biocompatible electrode...

Objective. Carbon nanotubes (CNTs) are attractive for use in peripheral nerve interfaces because of their unique combination strength, flexibility, electrical conductivity and nanoscale surface texture. Here we investigated the growth motor neurons on thin films horizontally aligned CNTs (HACNTs). Approach. We cultured primary embryonic rat HACNTs performed statistical analysis length orientation neurites. next presented with substrates alternating stripes SiO2. Main results. The survived...

This study compared epimysial patch electrodes with intramuscular hook using monopolar and bipolar recording configurations. The purpose was to determine which strategy transduced muscle signals better fidelity for control of myoelectric prostheses.One the two electrode styles, (n = 4) or 6) applied left extensor digitorum longus in rats. Electrodes were evaluated at time placement monthly intervals 4 months. Evaluations consisted evoked electromyography from stimulation pulses peroneal...

Electrospinning is a technique for producing micro- to nano-scale fibers. Fibers can be electrospun with varying degrees of alignment, from highly aligned completely random. In addition, fibers spun variety materials, including biodegradable polymers such as poly-L-lactic acid (PLLA). These characteristics make suitable scaffolding applications in tissue engineering. Our focus on the use nerve regeneration. We have previously shown that PLLA direct outgrowth both primary sensory and motor...

High-fidelity signal acquisition is critical for the fundamental control of a neuroprosthesis. Our group has developed bio-artificial interface consisting muscle graft neurotized by severed nerve in rat hind limb model. This regenerative peripheral (RPNI) permits transmission, amplification, and detection via situ electromyography (EMG). study examined magnitude interference from simultaneously contracting muscles adjacent to our interest. In eighteen F344 rats, extensor digitorum longus...