A5012453668- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- Force Microscopy Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Atomic and Molecular Physics
- Mechanical and Optical Resonators
- Mass Spectrometry Techniques and Applications
- Molecular Junctions and Nanostructures
- Fault Detection and Control Systems
- Bacteriophages and microbial interactions
- Nanopore and Nanochannel Transport Studies
- Supramolecular Chemistry and Complexes
- X-ray Spectroscopy and Fluorescence Analysis
- Plasma Diagnostics and Applications
- Genomics and Chromatin Dynamics
- Spam and Phishing Detection
- Advanced Chemical Physics Studies
- ATP Synthase and ATPases Research
- Machine Learning in Materials Science
Quantemol (United Kingdom)
2024
Madrid Institute for Advanced Studies
2018-2024
Universidad Autónoma de Madrid
2020
IMDEA Nanoscience
2020
Centro Nacional de Biotecnología
2018
Molecular shuttles are the basis of some most advanced synthetic molecular machines. In these devices a macrocycle threaded onto linear component between different portions thread in response to external stimuli. Here, we use optical tweezers measure mechanics and dynamics individual aqueous conditions. Using DNA as handle single molecule reporter, thousands shuttling events determine force-dependent kinetic rates motion main parameters governing energy landscape system. Our findings could...
Abstract Many replicative DNA polymerases couple replication and unwinding activities to perform strand displacement synthesis, a critical ability for metabolism. Strand is tightly regulated by partner proteins, such as single-stranded (ssDNA) binding proteins (SSBs) poorly understood mechanism. Here, we use single-molecule optical tweezers biochemical assays elucidate the molecular mechanism of synthesis human mitochondrial polymerase, Polγ, its modulation cognate noncognate SSBs. We show...
Genome replication induces the generation of large stretches single-stranded DNA (ssDNA) intermediates that are rapidly protected by DNA-binding (SSB) proteins. To date, mechanism which tightly bound SSBs removed from ssDNA lagging strand polymerase without compromising advance fork remains unresolved. Here, we aimed to address this question measuring, with optical tweezers, real-time kinetics human mitochondrial and bacteriophage T7 polymerases on free-ssDNA, in comparison covered...
The process of dissociation for two hydrofluorocarbon molecules in low triplet states excited by electron impact plasma is investigated ab initio molecular dynamics (AIMD). interest the hydrofluorocarbons motivated their role etching microelectronic technologies. Dissociation very fast, and reaction products can be predicted. In this work, it was found that higher relax into lowest state within a few femtoseconds due to nonadiabatic dynamics, such simplest MD on seems give reasonable...
Abstract Numerous measurements and calculations exist for total electron impact ionization cross sections. However, knowing fragmentation patterns is important in various scientific fields such as plasma physics, astrochemistry, environmental sciences. Partial sections can be calculated by multiplying with branching ratios different fragments, which deduced from mass spectra. the required spectrometry data frequently unavailable.
A machine learning-based method to predict spectra...
The process of dissociation for two hydrofluorocarbon molecules in low triplet states excited by electron impact plasma is investigated ab initio Molecular Dynamics (AIMD). interest hydrofluorocarbons motivated their role etching microelectronic technologies. Dissociation very fast, and the reaction products can be predicted. In this work, it was found that higher relax into lowest state within a few femtoseconds due to nonadiabatic dynamics, so simplest MD on seems give reasonable estimate...
The human mitochondrial replicative helicase, Twinkle, is essential for the replication and integrity of DNA (mtDNA). Therefore, investigating real-time kinetics Twinkle’s activities their regulation crucial understanding mtDNA maintenance. Here, we combine biochemical single-molecule manipulation visualization techniques to investigate loading Twinkle onto fork, its unwinding rewinding kinetics, these processes by amino- (N) carboxyl- (C) terminal ends helicase SSB protein (mtSSB). We...
Here, we exploit the high force (0.1 pN), spatial (1 nm) and temporal kHz) resolutions of optical tweezers to quantify control mechanically real-time kinetics individual synthetic molecular shuttles operating at near-physiological conditions, for several hundreds switching cycles, near equilibrium conditions.
Abstract Many replicative DNA polymerases couple replication and unwinding activities to perform strand displacement synthesis, a critical ability for metabolism. Strand is tightly regulated by partner proteins, such as single-stranded (ssDNA) binding proteins (SSBs) poorly understood mechanism. Here, we use single-molecule optical tweezers biochemical assays elucidate the molecular mechanism of synthesis human mitochondrial polymerase, Polγ, its modulation cognate noncognate SSBs. We show...
Here, we exploit the high force (0.1 pN), spatial (1 nm) and temporal kHz) resolutions of optical tweezers<a href="#_ENREF_4"><sup></sup></a><sup></sup><a href="#_ENREF_11"><sup></sup></a> to quantify control mechanically real-time kinetics individual synthetic molecular shuttles operating at near-physiological conditions, for several hundreds switching cycles, near equilibrium conditions.