A5025527652- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Spinal Fractures and Fixation Techniques
- Cardiac electrophysiology and arrhythmias
- Spine and Intervertebral Disc Pathology
- Cervical and Thoracic Myelopathy
- Chemical Synthesis and Analysis
- Plant Reproductive Biology
- Adaptive Control of Nonlinear Systems
- HIV/AIDS drug development and treatment
- Plant Molecular Biology Research
- Metal and Thin Film Mechanics
- GaN-based semiconductor devices and materials
- Robotic Path Planning Algorithms
- Nicotinic Acetylcholine Receptors Study
- Control and Dynamics of Mobile Robots
- Asymmetric Synthesis and Catalysis
- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Synthetic Organic Chemistry Methods
- Computational Drug Discovery Methods
- Aerospace Engineering and Control Systems
- Medical Imaging and Analysis
- DNA and Nucleic Acid Chemistry
- Plant Stress Responses and Tolerance
Tokyo Medical and Dental University
2020-2024
Nagoya University
1989-2024
Sapporo Higashi Tokushukai Hospital
2023-2024
Osaka University of Economics
2023
Okayama University
2023
Okayama University Hospital
2023
Western Region Agricultural Research Center
2015-2021
Shibaura Institute of Technology
2020-2021
Graduate School USA
2020
Teikyo University of Science
2019
cDNA clones for four different N-methyl-D-aspartate (NMDA) receptor subunits (NMDAR2A-NMDAR2D) were isolated through polymerase chain reactions followed by molecular screening of a rat brain library. These are only about 15% identical with the key subunit NMDA (NMDAR1) but highly homologous (approximately 50% homology) one another. They also commonly possess large hydrophilic domains at both amino- and carboxyl-terminal sides putative transmembrane segments. NMDAR2A NMDAR2C expressed...
An intermediate conductance calcium-activated potassium channel, hIK1, was cloned from human pancreas. The predicted amino acid sequence is related to, but distinct from, the small channel subfamily, which ≈50% conserved. hIK1 mRNA detected in peripheral tissues not brain. Expression of Xenopus oocytes gave rise to inwardly rectifying currents, were activated by submicromolar concentrations intracellular calcium ( K 0.5 = 0.3 μM). Although for similar that channels, slope factor derived Hill...
We cloned a cDNA (HAC4) that encodes the hyperpolarization-activated cation channel (I for I h) by screening rabbit sinoatrial (SA) node library using fragment of rat brainI f cDNA. HAC4 is composed 1150 amino acid residues, and its cytoplasmic N- C-terminal regions are longer than those HAC1–3. The transmembrane region was most homologous to partially mouse BCNG-3 (96%), whereas showed low homology all HAC family members so far cloned. Northern blotting revealed mRNA highly expressed in SA...
Small conductance calcium-activated potassium channels show a distinct pharmacology. Some, but not all, are blocked by the peptide toxin apamin, and apamin-sensitive also <i>d</i>-tubocurarine. Cloned SK (small channel) recapitulate these properties. We have investigated structural basis for differences found that two amino acid residues on either side of deep pore primary determinants sensitivity to apamin differential block Therefore, pharmacology compared with other correlates in outer...
We have isolated a novel inward rectifier K+ channel predominantly expressed in glial cells of the central nervous system. Its amino acid sequence exhibited 53% identity with ROMK1 and approximately 40% other channels. Xenopus oocytes injected cRNA derived from this clone current, which showed classical characteristics. Intracellular Mg.ATP was required to sustain activity excised membrane patches, is consistent Walker type-A ATP-binding domain on clone. designate new as KAB-2 (the second...
Kinematics of the upper cervical spine during head rotation were investigated using three-dimensional magnetic resonance imaging (MRI) in healthy volunteers.To demonstrate vivo intervertebral coupled motions spine.Although various and vitro studies have identified normal movement patterns spine, no previous accurately analyzed rotation.Fifteen volunteers underwent MRI a 1.0-T imager progressive 15 degrees steps rotation. Segmented MRIs each vertebra neutral position superimposed over images...
Despite its importance in plant growth and development, the auxin biosynthetic pathway has remained elusive. In this study, we analyzed hormone series transcriptome data from AtGenExpress Arabidopsis found that aminoethoxyvinylglycine (AVG) had strongest anti-auxin activity. We also identified other effective compounds such as l-amino-oxyphenylpropionic acid (AOPP) through additional screening. These inhibitors shared characteristics they inhibited pyridoxal enzymes and/or aminotransferases....
Abstract COVID-19 caused by SARS-CoV-2 has continually been serious threat to public health worldwide. While a few anti-SARS-CoV-2 therapeutics are currently available, their antiviral potency is not sufficient. Here, we identify two orally available 4-fluoro-benzothiazole-containing small molecules, TKB245 and TKB248, which specifically inhibit the enzymatic activity of main protease (M pro ) significantly more potently block infectivity replication various strains than nirmatrelvir,...
In Brief Study Design. Kinematics of the cervical spine during lateral bending were investigated using a novel system three-dimensional motion analysis. Objectives. To demonstrate in vivo intervertebral coupled motions neck. Summary Background Data. No previous studies have successfully documented bending. Methods. Twelve healthy volunteers underwent magnetic resonance imaging (MRI) 7 positions with 10° increments Relative calculated automatically by superimposing segmented...
Background: Total en bloc spondylectomy (TES) was devised to minimize the incidence of local recurrence following resection spinal tumor. Successful control with TES has been reported for patients primary malignant or aggressive benign tumors. As metastatic tumors, however, only a few surgeons except inventor group have outcome TES. The purpose this study investigate whether could provide radical tumor in solitary metastases. Methods: Twelve underwent thoracolumbar spine. Primary...
Auxin is essential for plant growth and development, this makes it difficult to study the biological function of auxin using auxin-deficient mutants. Chemical genetics have potential overcome difficulty by temporally reducing inhibitors. Recently, indole-3-pyruvate (IPyA) pathway was suggested be a major biosynthesis in Arabidopsis thaliana L. indole-3-acetic acid (IAA), most common member family. In pathway, YUCCA, flavin-containing monooxygenase (YUC), catalyzes last step conversion from...
The main protease (Mpro) of SARS-CoV-2 is an attractive target for the development drugs to treat COVID-19. Here, we report design, synthesis, and structure-activity relationship (SAR) studies highly potent Mpro inhibitors including TKB245 (5)/TKB248 (6). Since have previously developed (3) (4), several hybrid molecules these previous compounds combined with nirmatrelvir (1) were designed synthesized. Compounds such as (5) TKB248 (6), containing a 4-fluorobenzothiazole moiety at P1' site,...
1. The structural basis for the different activation kinetics of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels was investigated with whole-cell patch clamp technique by using HCN1, HCN4, chimeric and mutants in a mammalian expression system (COS-7). 2. time constant HCN4 about 40-fold longer than that HCN1 when compared at -100 mV. 3. In chimeras between region S1 transmembrane domain exoplasmic S1-S2 linker markedly affected kinetics. cytoplasmic S6 nucleotide-binding...
With the completion of determination its entire genome sequence, one next major targets Bacillus subtilis genomics is to clarify whole gene regulatory network. To this end, results systematic experiments should be compared with rich source individual experimental accumulated so far. Thus, we constructed a database upstream information B.subtilis (DBTBS). The current version was by surveying 291 references and contains on 90 binding factors 403 promoters. For each promoter, all known...
In Brief Study Design. Three-dimensional intervertebral motions of the subaxial cervical spine during head rotation were investigated in healthy volunteers using three-dimensional magnetic resonance imaging (MRI). Objectives. To document coupled rotation. Summary Background Data. vivo kinematics have not previously been well described, since they are too complicated to follow conventional radiography or computed tomography techniques. Methods. Ten underwent MRI 11 positions with 15°...
A retrospective study was conducted.To compare the long-term outcomes after laminoplasty and anterior spinal fusion (ASF) for cervical myelopathy secondary to disc herniation.There have been no reports of comparative studies ASF due herniation.Of 21 patients who underwent only between 1984 1987, 15 were followed up. Of 22 1987 1994, 18 There significant differences in preoperative prognostic factors 2 groups. Average follow-up years group 10 group. Neurologic radiologic results...
Abstract Regeneration of cardiac pacemakers is an important target regeneration. Previously, we developed a novel embryonic stem (ES) cell differentiation system that could trace cardiovascular processes at the cellular level. In present study, examine expressions and functions ion channels in ES cell-derived cardiomyocytes during their identify confer automaticity. Flk1+ mesoderm cells give rise to spontaneously beating on OP9 stroma cells. Spontaneously colonies observed day 9.5 culture...