A5018829264- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cystic Fibrosis Research Advances
- Cancer Research and Treatments
- Antimicrobial Peptides and Activities
- Microbial Natural Products and Biosynthesis
- RNA Interference and Gene Delivery
- Pharmaceutical studies and practices
- Microbial Metabolites in Food Biotechnology
- Respiratory viral infections research
- Pneumocystis jirovecii pneumonia detection and treatment
- Monoclonal and Polyclonal Antibodies Research
- Pediatric health and respiratory diseases
- Bacterial biofilms and quorum sensing
- MicroRNA in disease regulation
- Pain Management and Placebo Effect
- Antibiotic Resistance in Bacteria
- Redox biology and oxidative stress
- Liver Disease and Transplantation
- Genomics and Phylogenetic Studies
- Neuroscience, Education and Cognitive Function
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Liver physiology and pathology
SQZ Biotech (United States)
2017-2022
University of Pennsylvania
2014-2015
Tufts University
2010-2011
ABSTRACT Pseudomonas aeruginosa Liverpool epidemic strain (LES) infections in cystic fibrosis (CF) patients are associated with transmissibility and increased patient morbidity. This study was designed to assess the vitro activities of cathelicidin LL-37 peptide (LL-37) select cationic lipids against LESB58 CF sputum a setting mimicking airway. We found that naturally present airway surface fluid some nonpeptide lipid molecules such as CSA-13, CSA-90, CSA-131, D2S have significant, but...
Abstract CD8+ T cell responses are the foundation of recent clinical success immunotherapy in oncologic indications. Although checkpoint inhibitors have enhanced activity existing responses, therapeutic approaches to generate Ag-specific had limited success. Here, we demonstrate that cytosolic delivery Ag through microfluidic squeezing enables MHC class I presentation cells by diverse types. In murine dendritic (DCs), squeezed DCs were ∼1000-fold more potent at eliciting than...
MicroRNAs (miRNAs) can control stem cell differentiation by targeting mRNAs. Using 96-well plate electroporation, we screened 466 human miRNA mimics four-color flow cytometry to explore of common myeloid progenitors (CMP) derived from embryonic cells (hESCs). The transfected were then cultured in a cytokine cocktail that supported multiple hematopoietic lineages. At 4-5 days post-transfection, erythroid (CD235(+)CD41(-)), megakaryocyte (CD41(+)CD42(+)), and (CD18(+)CD235(-)) lineages...
Patients with cystic fibrosis (CF) often suffer chronic lung infection concomitant inflammation, a setting that may reduce the efficacy of gene transfer. While therapy development for CF involves viral-based vectors, little is known about transfer in context an infected airway. In this study, three mouse models were established to evaluate adeno-associated virus (AAV) such environment. Bordetella bronchiseptica RB50 was used chronic, nonlethal respiratory C57BL/6 mice. An inoculum ∼10(5) CFU...
Abstract The partitioning of pristinamycins was studied in dextran and polyethylene glycol (PEG) aqueous two‐phases systems. Pristinamycins partitioned preferentially into the PEG‐rich top phase. partition coefficient independent molar mass PEG antibiotic concentration, but, increased exponentially with tieline length system. Partition greatly improved when fatty acids esters were mixed PEG. In such mixtures, up to a value 24, dependent on carbon chain modified concentrations. Moreover,...
Journal Article Construction and performance of heterologous polyketide‐producing K‐12‐ B‐derived Escherichia coli Get access J. Wu, Wu State Key Laboratory Bioreactor Engineering, National Engineering Research Center for Biotechnology, East China University Science & Technology, Shanghai, Department Chemical Biological Technology Center, Tufts University, Medford, MA, USA Search other works by this author on: Oxford Academic Google Scholar B.A. Boghigian, Boghigian M. Myint, Myint H. Zhang,...
Activation of T cell responses is essential for effective tumor clearance; however, inducing targeted, potent antigen presentation to stimulate remains challenging. We generated Activating Antigen Carriers (AACs) by engineering red blood cells (RBCs) encapsulate relevant antigens and the adjuvant polyinosinic-polycytidylic acid (poly I:C), use as a tumor-specific cancer vaccine. The processing method conditions used create AACs promote phosphatidylserine exposure on RBCs thus harness natural...
<h3>Background</h3> Antigen-specific CD8+ T cell activity is critical for mounting an effective immune response in a wide range of indications, including immune-oncology and infectious diseases. <h3>Methods</h3> To elicit antigen-specific activity, we used microfluidics squeezing (Cell Squeeze®) to deliver antigens directly the cytosol antigen presenting cells (APCs). Direct cytosolic delivery bypasses need cross-presentation efficiently loads into major histocompatibility complex class I...
<h3>Background</h3> We engineered unfractionated peripheral blood mononuclear cells (PBMCs) to function as antigen presenting (APCs) that generate potent CD8+ T cell responses. investigated the combined efficacy of PBMC-based cancer vaccine with targeted interleukin 2 variants (IL2v); anti-Programmed Cell Death Protein 1 (muPD1-IL2v) and anti-Fibroblast Activation (muFAP-IL2v). <h3>Methods</h3> generated microfluidic engineering system (Cell Squeeze®), which facilitates direct cytosolic...
Background: CD8 T cells are critical to immunotherapeutic intervention in cancer. However, the ability efficiently target antigens for MHC-I presentation has limited efficacy of immunization strategies. Here, we use microfluidics-based SQZ cell therapy platform deliver antigen cytosol APCs, resulting enhanced on MHC-I. Given that no expansion time is required engineer these this approach allows rapid manufacturing billions non-conventional APCs capable priming CD8+ responses. Methods:...
<h3>Background</h3> Despite considerable advances in cancer immunotherapy, it has proven difficult to effectively target 'cold' tumors with limited immune infiltration. Chimeric antigen receptor T cells (CAR-T cells) are one potential solution; however, their clinical use been confined treating hematologic malignancies partly due physical exclusion the context of solid tumors. Cytokine-based therapy shown significant pre-clinical models, yet its utility marked by efficacy and toxicities....
<h3>Background</h3> Despite considerable progress in immunotherapy, the challenge persists effectively targeting immunologically 'cold' tumors. Cytokine-driven therapy has exhibited promise; however, its integration into clinical practice been hindered by modest efficacy and adverse effects. Moreover, this strategy is not cytotoxic, limiting antigen availability for uptake presentation immune system. Limited studies have evaluated methods enhancing expression release of intrinsic...
Abstract In recent years, ex vivo manipulation of primary cells has shown immense clinical potential with the advent adoptive T cell therapies. Conventional methods for manipulation, however, are not without limitations. They typically rely on application electrical fields or exogenous materials such as viral vectors and plasmids, which can increase cellular toxicity off-target effects. To overcome limitations, we have developed an approach using our CellSqueeze Technology that causes...