A5088056432- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Nitric Oxide and Endothelin Effects
- Gut microbiota and health
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Inflammasome and immune disorders
- Cancer Immunotherapy and Biomarkers
- Vitamin D Research Studies
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Lipid metabolism and disorders
- Digestive system and related health
- Xenotransplantation and immune response
- T-cell and B-cell Immunology
- Blood disorders and treatments
- Magnetic and Electromagnetic Effects
- IL-33, ST2, and ILC Pathways
- Inflammatory Biomarkers in Disease Prognosis
SQZ Biotech (United States)
2020-2022
National Institutes of Health
2014-2019
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2014-2019
National Cancer Institute
2017-2018
Center for Cancer Research
2017-2018
University of Florida
2017
University of Michigan
2012-2015
Ann Arbor Center for Independent Living
2014
The University of Texas at Austin
2010
Michigan Department of Agriculture and Rural Development
1957
Neutrophil extracellular traps (NETs) represent an important defense mechanism against microorganisms. Clearance of NETs is impaired in a subset patients with systemic lupus erythematosus, and NETosis increased neutrophils and, particularly, low-density granulocytes derived from patients. are toxic to the endothelium, expose immunostimulatory molecules, activate plasmacytoid dendritic cells, may participate organ damage through incompletely characterized pathways. To better understand role...
Objectives An imbalance between neutrophil extracellular trap (NET) formation and degradation has been described in systemic lupus erythematosus (SLE), potentially contributing to autoantigen externalisation, type I interferon synthesis endothelial damage. We have demonstrated that peptidylarginine deiminase (PAD) inhibition reduces NET protects against lupus-related vascular damage the New Zealand Mixed model of lupus. However, another strategy for inhibiting NETs—knockout NOX2 —accelerates...
Dysregulation of innate and adaptive immune responses contributes to the pathogenesis systemic lupus erythematosus (SLE) its associated premature vascular damage. No drug date targets both inflammatory disease cardiovascular complications SLE. Tofacitinib is a JAK inhibitor that blocks signaling downstream multiple cytokines implicated in pathogenesis. While clinical trials have shown tofacitinib exhibits significant efficacy various autoimmune diseases, role SLE pathology remains be...
Objective Oxidative stress and oxidized high‐density lipoprotein (HDL) are implicated as risk factors for cardiovascular disease (CVD) in systemic lupus erythematosus (SLE). Yet, how HDL is rendered dysfunctional SLE remains unclear. Neutrophil extracellular traps (NETs), the levels of which elevated lupus, possess oxidant‐generating enzymes, including myeloperoxidase (MPO), NADPH oxidase (NOX), nitric oxide synthase (NOS). We hypothesized that NETs mediate oxidation, impairing cholesterol...
Abstract Objective Patients with systemic lupus erythematosus (SLE) have a notable increase in atherothrombotic cardiovascular disease (CVD) which is not explained by the Framingham risk equation. In vitro studies indicate that type I interferons (IFNs) may play prominent roles increased CV SLE. However, vivo relevance of these findings, regard to development CVD, has been characterized. This study was undertaken examine role IFNs endothelial dysfunction, aberrant vascular repair, and...
Objectives The presence of proinflammatory low-density granulocytes (LDG) has been demonstrated in autoimmune and infectious diseases. Recently, regulatory neutrophilic polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) were identified systemic lupus erythematosus (SLE). Because LDG PMN-MDSC share a similar phenotype with contrasting functional effects, we explored these cohort patients SLE. Methods normal-density (NDG) isolated from fresh blood healthy donors (HD) Associations...
Rationale: Autologous and allogeneic hematopoietic stem cell transplant (HSCT) patients are susceptible to pulmonary infections, including bacterial pathogens, even after reconstitution. We previously reported that murine bone marrow (BMT) neutrophils overexpress cyclooxygenase-2, overproduce prostaglandin E2 (PGE2), exhibit defective intracellular killing. Neutrophil extracellular traps (NETs) DNA structures capture kill bacteria other pathogens.Objectives: To determine whether NETosis was...
Recent evidence indicates that high-density lipoprotein (HDL) exerts vasculoprotective activities by promoting activating transcription factor 3 (ATF3), leading to downregulation of toll-like receptor (TLR)-induced inflammatory responses. Systemic lupus erythematosus (SLE) is associated with increased cardiovascular disease risk not explained the Framingham score. studies have indicated oxidised HDL as a possible contributor. We investigated potential mechanisms which may lose its...
To identify a genetic cause of early-onset systemic lupus erythematosus (SLE) in large consanguineous family from Turkey and to study the mechanisms disease.
Patients with systemic lupus erythematosus (SLE) have an increased risk of cardiovascular disease (CVD) and impaired endothelial repair. Although vitamin D deficiency is associated CVD in the general population, a causal relationship has not been demonstrated. We aimed to determine whether directly modulates dysfunction immune responses murine model SLE.Vitamin was induced lupus-prone MRL/lpr mice by dietary restriction for 6 weeks. Endothelium-dependent vasorelaxation quantified using...
Abstract CD8+ T cell responses are the foundation of recent clinical success immunotherapy in oncologic indications. Although checkpoint inhibitors have enhanced activity existing responses, therapeutic approaches to generate Ag-specific had limited success. Here, we demonstrate that cytosolic delivery Ag through microfluidic squeezing enables MHC class I presentation cells by diverse types. In murine dendritic (DCs), squeezed DCs were ∼1000-fold more potent at eliciting than...
Objective. SLE is an autoimmune disease characterized by autoantibody generation, organ damage and increased risk of cardiovascular disease. Generally considered anti-inflammatory cytokine, IL-10 in correlates with poor outcomes the general population. The aim this study was to explore putative role modulating endothelial function examining effects cytokine on progenitor cell/circulating angiogenic cell (EPC/CAC) differentiation.
The use of an indicator displacement assay permits the visualization binding events between host and guest molecules. An undergraduate laboratory experiment is described to demonstrate technique in determination citric acid content commercially available beverages such as soda pop fruit juices. Through technique, students can learn supramolecular analytical chemistry concepts via a practical, real-world application.
The human microbiota maintains an enormous and diverse capacity to produce a diet-dependent metabolome that impacts both host tissue microbial community homeostasis. Recent discoveries support growing appreciation metabolites derived from bioactive foods are also important regulators of immune metabolic functions. To gain better understanding the current evidence for roles dietary in tumor immunity, Division Cancer Biology Prevention, National Institute, cosponsored workshop on August 31...
Activation of T cell responses is essential for effective tumor clearance; however, inducing targeted, potent antigen presentation to stimulate remains challenging. We generated Activating Antigen Carriers (AACs) by engineering red blood cells (RBCs) encapsulate relevant antigens and the adjuvant polyinosinic-polycytidylic acid (poly I:C), use as a tumor-specific cancer vaccine. The processing method conditions used create AACs promote phosphatidylserine exposure on RBCs thus harness natural...
<h3>Background</h3> Antigen-specific CD8+ T cell activity is critical for mounting an effective immune response in a wide range of indications, including immune-oncology and infectious diseases. <h3>Methods</h3> To elicit antigen-specific activity, we used microfluidics squeezing (Cell Squeeze®) to deliver antigens directly the cytosol antigen presenting cells (APCs). Direct cytosolic delivery bypasses need cross-presentation efficiently loads into major histocompatibility complex class I...
Abstract Lupus (SLE) patients are fifty times more likely to experience a cardiovascular event compared age- and sex-matched controls. High levels of oxidized HDL (oxHDL) in SLE may contribute this association. Under normal conditions, unoxidized induces an anti-inflammatory response within macrophages (MØ). We addressed whether lupus oxHDL modifies putative mechanisms associated with enhanced inflammation. Methods: Affymetrix gene expression analysis was performed on control MØs exposed or...