A5003594702- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- CRISPR and Genetic Engineering
- Microfluidic and Bio-sensing Technologies
- Micro and Nano Robotics
- Virus-based gene therapy research
- Ion-surface interactions and analysis
- Fuel Cells and Related Materials
- 3D Printing in Biomedical Research
- Molecular Junctions and Nanostructures
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Advanced biosensing and bioanalysis techniques
- Advanced Polymer Synthesis and Characterization
- Nanoparticle-Based Drug Delivery
- Electrochemical sensors and biosensors
- Electron and X-Ray Spectroscopy Techniques
- Advanced Sensor and Energy Harvesting Materials
- Drug-Induced Hepatotoxicity and Protection
- Pickering emulsions and particle stabilization
- T-cell and B-cell Immunology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Particle Dynamics in Fluid Flows
- Immune cells in cancer
SQZ Biotech (United States)
2016-2023
Massachusetts Institute of Technology
2010-2015
DePaul University
2015
Cyprotex (United States)
2015
Howard Hughes Medical Institute
2010
Johns Hopkins University
2009
Genetic Information Research Institute
2002
Functional organic thin films often demand precise control over the nanometer-level structure. Interlayer diffusion of materials may destroy this structure; therefore, a better understanding when interlayer occurs and how to it is needed. X-ray photoelectron spectroscopy paired with C 60 + cluster ion sputtering enables high-resolution analysis atomic composition chemical state depth. Using technique, we explore issues common polyelectrolyte multilayer field, such as competition between...
We demonstrate a reduction in the measured inner wall shear stress moderately turbulent Taylor-Couette flows by depositing sprayable superhydrophobic microstructures on rotor surface. The magnitude of becomes progressively larger as Reynolds number increases up to value 22% at $\mathrm{Re}=8.0\ifmmode\times\else\texttimes\fi{}1{0}^{4}$. show that mean skin friction coefficient ${C}_{f}$ presence coating can be fitted modified Prandtl--von K\'arm\'an--type relationship form...
Macrophages are recruited at the diseased site in several pathological conditions. Here, we describe a novel method of utilizing their unique properties for targeted drug delivery. Cellular backpacks designed that ride on macrophage surfaces without affecting cell functions or getting internalized. These can be used as multimodal therapeutic and diagnostic agents.
The translational potential of cell-based therapies is often limited by complications related to effectively engineering and manufacturing functional cells. While the use electroporation widespread, impact on cell state function has yet be fully characterized. Here, we a genome-wide approach study optimized treatment identify striking disruptions in expression profiles key transcripts human T These genetic result concomitant perturbation cytokine secretion including 648-fold increase IL-2 (P...
X-ray photoelectron spectroscopy (XPS) depth profiling with C60(+) sputtering was used to resolve the lithium-ion distribution in nanometer-scale domain structures of block polymer electrolyte thin films. The electrolytes interest are mixtures lithium trifluoromethanesulfonate and lamellar-forming polystyrene-poly(oligo(oxyethylene)methacrylate) (PS-POEM) copolymer. XPS results showed that concentration directly correlated POEM concentration. Furthermore, chemical state atomic composition...
Manipulating surface properties using chemistry and roughness has led to the development of advanced multifunctional surfaces. Here, in a nanostructured polymer film consisting hydrophilic reservoir chitosan/carboxymethyl cellulose capped with various hydrophobic layers, we demonstrate role third design factor, water permeation rate. We use this additional criterion produce antifogging coatings that readily absorb vapor while simultaneously exhibiting character liquid water. These...
Cellular "backpacks" are a new type of anisotropic, nanoscale thickness microparticle that may be attached to the surface living cells creating "bio-hybrid" material. Previous work has shown these backpacks do not impair cell viability or native functions such as migration in B and T line, respectively. In current work, we show backpacks, when added suspension, assemble into aggregates reproducible size. We investigate efficiency backpack-cell binding using flow cytometry laser diffraction,...
Tubular particles presenting heterogeneous regions of chemistry on the tube-ends versus side are fabricated and shown to control particle orientation surface live lymphocytes. Controlling anisotropic microparticles cell surfaces is interest for biomedical applications drug delivery in particular, since it can be used promote or resist internalization.
Biochemical screening is a major source of lead generation for novel targets. However, during the process small molecule optimization, compounds with excellent biochemical activity may show poor cellular potency, making structure–activity relationships difficult to decipher. This be due low membrane permeability molecule, resulting in insufficient intracellular drug concentration. The Cell Squeeze platform increases regardless compound structure by mechanically disrupting membrane, which can...
We demonstrate that noncovalent ion-pair interactions in solution can be employed to control the molecular spacing of thiols a self-assembled monolayer (SAM) on gold. Ion-pairs formed between carboxylate tail-group 16-mercaptohexadecanoic acid (MHA) and tetraalkylammonium (TAA+) hydroxide salts various alkyl side-chain lengths remain intact during chemisorption thiol The resulting SAMs exhibit 1:1 molar ratio MHA:TAA+ surface are covalently bound gold through headgroup MHA. hypothesize...
Inhibition of hepatic efflux transporters (BSEP, MRP2) is one the important mechanisms in drug-induced liver injury and particular cholestasis. The inhibitory effect compounds on these two commonly delineated using inverted membrane vesicles from Sf9 insect cells overexpressing BSEP or MRP2 protein. However, due to lack bioactivation metabolism compound, expression functionality other sinusoidal uptake cell-free assay system, it difficult fully understand role a given transporter Therefore,...
It is demonstrated that poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/SPS) multilayer films can be successfully tailored for the capture and detection of small biomolecules in dilute concentrations. Based on vitro results, these could potentially applied rapid high-throughput diagnosis biomarkers serum or tissue. PAH presents functional amino groups further reacted with desired chemistries order to create customizable specific surfaces biomolecule capture. A variety film...
Activation of T cell responses is essential for effective tumor clearance; however, inducing targeted, potent antigen presentation to stimulate remains challenging. We generated Activating Antigen Carriers (AACs) by engineering red blood cells (RBCs) encapsulate relevant antigens and the adjuvant polyinosinic-polycytidylic acid (poly I:C), use as a tumor-specific cancer vaccine. The processing method conditions used create AACs promote phosphatidylserine exposure on RBCs thus harness natural...
While the ex vivo manipulation of primary cells has signaled a new era in application cell-based therapies, common methods to manipulate have limitations. To overcome limitations associated with conventional cell delivery and engineering systems, we developed microfluidic approach where are mechanically deformed as they pass through constricting channels. This deforms membrane resulting diffusion material from surrounding buffer directly into cytosol. system demonstrated efficacy...
Abstract Ex vivo manipulation of the immune system for therapeutic purposes has shown incredible clinical promise with advent cell therapies such as Chimeric Antigen Receptor modified T-Cell Therapies (CAR-T). However expanding methods to manipulate cells beyond using plasmids and viruses requires a new delivery paradigm. Here we describe microfluidic approach discovered at MIT where are mechanically deformed they pass through constriction smaller than diameter. The resulting controlled...
Abstract Intracellular delivery of molecules is a key step in biological research that enables the development new methods cell and gene-based therapies. Traditional for intracellular are limited a) delivering wide variety cargos, b) efficient to broad range types, c) preventing unwanted genetic dysregulation. To address these challenges, we have developed CellPore™ system, benchtop device gentle, yet cargos primary types. The system microfluidic platform creates transient disruptions plasma...
Abstract The ex vivo manipulation of primary cells is critical to an emerging generation cell-based therapies, such as chimeric antigen receptor systems and CRISPR mediated genomic editing. However, the limitations existing methods for delivering desired material interest could dramatically hinder development impact these therapies. To overcome challenges associated with conventional cell delivery engineering systems, we have developed a microfluidic approach, CellSqueeze®, where are...