A5045089998- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Fibroblast Growth Factor Research
- Genetic and phenotypic traits in livestock
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Kruppel-like factors research
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Ovarian function and disorders
- Effects of Environmental Stressors on Livestock
- Molecular Biology Techniques and Applications
- Helicobacter pylori-related gastroenterology studies
- Bioenergy crop production and management
- Renal and related cancers
- Adipose Tissue and Metabolism
- Hormonal and reproductive studies
- Growth Hormone and Insulin-like Growth Factors
- Pregnancy and preeclampsia studies
- Tissue Engineering and Regenerative Medicine
- Endometriosis Research and Treatment
- Assisted Reproductive Technology and Twin Pregnancy
- Cytokine Signaling Pathways and Interactions
- Milk Quality and Mastitis in Dairy Cows
- Cardiovascular Effects of Exercise
Universidade do Oeste Paulista
2016-2025
Faculdade São Leopoldo Mandic
2024
Universidade Estadual Paulista (Unesp)
2011-2023
Universidade de São Paulo
2011-2021
Aim To investigate whether high-intensity interval training (HIIT) improves physical performance, systolic blood pressure, and markers of oxidative stress inflammation in skeletal muscle spontaneously hypertensive rats (SHR). Methods Nineteen male SHR were randomly assigned to two groups: sedentary (SHRC) trained (SHR+T). The SHR+T group five times a week for eight weeks on treadmill, while the remained without any exercise stimulus throughout experimental period. Maximum performance...
Some fibroblast growth factors (FGFs) affect ovarian follicle cell and/or differentiation. Whereas many FGFs activate several FGF receptors, FGF7 and FGF10 primarily only one, FGFR2B. As is produced by bovine theca cells acts on granulosa cells, we tested the hypothesis that may also play a role in folliculogenesis cattle. Reverse transcription-polymerase chain reaction demonstrated presence of mRNA oocytes antral follicles, as well preantral follicles. protein was detected...
Heat stress is an important cause of poor development and low survival rates in bovine embryos. Experiments were conducted to test the hypothesis that Bos indicus embryos are more resistant heat than taurus In experiment 1, Nelore Jersey from oocyte pick-up-derived oocytes submitted (96 hours post-insemination, 41 °C, 6 hours), developmental ratios assessed at Day 7 (Day 0 = day fertilization), blastocysts frozen for RNA extraction. Experiment 2 evaluated expression COX2, CDX2, HSF1, PLAC8...
Abstract In cattle, most evidence suggests that granulosa cells express LH receptors (LHR) after (or as) the follicle becomes dominant, however there is some suggestion from smaller pre‐dominant follicles may several LHR mRNA splice variants. The objective of this study was to measure expression in bovine defined size and steroidogenic ability, small (<6 mm diameter) undergoing differentiation vitro. Semiquantitative RT‐PCR demonstrated undetectable <7 diameter (nondominant follicles),...
To evaluate whether the joint use of autologous platelet-rich plasma (aPRP) and rosuvastatin (RSV) in biopsies dermal wounds induced rabbits results an additive effect on immunoexpression collagens type I III, optimizing healing process increasing collagen production during proliferative phase to improve quality tissue repair. Thirty-two biopsy samples from eight clinically healthy adult male New Zealand were used. They treated with aPRP, RSV, or aPRP + RSV analyzed zero, three, seven, ten,...
Multiple ovulation (superovulation) and embryo transfer has been used extensively in cattle. In the past decade, superstimulatory treatment protocols that synchronise follicle growth ovulation, allowing for improved donor management fixed-time AI (FTAI), have developed zebu (Bos indicus) European taurus) breeds of There is evidence additional stimulus with LH (through administration exogenous or equine chorionic gonadotrophin (eCG)) on last day protocol, called 'P-36 protocol' FTAI, can...
The present study determined the transcriptome profile in Nelore and Holstein oocytes subjected to heat shock during IVM mRNA abundance of selected candidate genes heat-shocked cumulus cells (CC). cows were vivo follicle aspiration. Cumulus-oocyte complexes assigned control (38.5°C, 22h) or (41°C for 12h, followed by 38.5°C 10h) treatment IVM. Denuded bovine microarray analysis. Transcriptome analysis demonstrated 127, nine six differentially expressed between breed, temperature...
Abstract There is evidence that several fibroblast growth factors (FGFs) are involved in and development of the corpus luteum (CL), but many FGFs have not been investigated this tissue, including FGF10. The objective study was to determine if FGF10 its receptor (FGFR2B) expressed CL. Bovine CL were collected from an abattoir classed as hemorrhagica (stage I), developing II), developed III), regressed IV) Expression FGFR2B mRNA measured by reverse transcription‐polymerase chain reaction...
Contents Fibroblast growth factor (FGF10) acts at the cumulus oocyte complex, increasing expression of cell expansion‐related genes and competency genes. We tested hypothesis that addition FGF10 to maturation medium improves maturation, decreases percentage apoptotic oocytes increases development blastocyst stage while relative abundance developmentally important ( COX2, CDX2 PLAC8 ). In all experiments, were matured for 22 h in TCM‐199 supplemented with 0, 2.5, 10 or 50 ng/ml FGF10....
Intrauterine dietary restriction may cause changes in the functioning of offspring organs and systems later life, an effect known as fetal programming. The present study evaluated mRNA abundance immunolocalization nutrient transporters well enterocytes proliferation proximal, median distal segments small intestine rats born to protein-restricted dams. Pregnant were fed hypoproteic (6% protein) or control (17% diets, at 3 16 weeks age. presence SGLT1 (sodium-glucose co-transporter 1), GLUT2...
There is evidence that fibroblast growth factors (FGFs) are involved in the regulation of and regression corpus luteum (CL). However, expression pattern most FGF receptors (FGFRs) during CL lifespan still unknown. The objective present study was to determine ‘B’ ‘C’ splice variants FGFRs bovine CL. Bovine were collected from an abattoir classed as corpora hemorrhagica (Stage I), developing II), developed III) or regressed IV) Expression FGFR mRNA measured by semiquantitative reverse...
Mammalian oocytes resume meiosis spontaneously after removal from the ovarian follicle. We tested effects of a 2-h prematuration treatment (Pre-IVM) with forskolin (FSK) and 3-isobutyl-1-methylxanthine (IBMX) in bovine cumulus-oocyte complexes (COCs) on lipid content blastocysts, membrane composition blastocysts transcriptional profiling cumulus cells high-throughput platform. Embryonic development rates to morula (mean 56.1%) or blastocyst 26.3%) stages were unaffected by treatment. Lipid...
There is evidence that regulation of follicle selection in cattle involves locally produced growth factors. In the present study, we investigated expression members fibroblast factor (FGF) 7 family during deviation. The largest and second follicles were recovered day a synchronised wave future dominant subordinate identified based on diameter cytochrome P450, 19, subfamily A, polypeptide 1 (CYP19A1) mRNA levels granulosa cells. Theca cells contained less encoding FGF7 FGF10 compared with...