A5013400454- Chronic Lymphocytic Leukemia Research
- Enzyme Structure and Function
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Drug-Induced Ocular Toxicity
- Signaling Pathways in Disease
- Polyamine Metabolism and Applications
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Autoimmune Neurological Disorders and Treatments
- Peptidase Inhibition and Analysis
- Herpesvirus Infections and Treatments
- Synthesis and biological activity
- CAR-T cell therapy research
- Advanced Breast Cancer Therapies
- 14-3-3 protein interactions
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Protein Kinase Regulation and GTPase Signaling
- Diabetes and associated disorders
- Enzyme Catalysis and Immobilization
- Melanoma and MAPK Pathways
- S100 Proteins and Annexins
Indian Institute of Technology Bombay
2013-2021
La Jolla Institute for Immunology
2017-2019
Indian Institute of Technology Indore
2013
Abstract Rassf1A/5 tumor suppressors serve as adaptor proteins possessing a modular architecture with the C-terminal consisting of coiled-coil SARAH (Salvador-Rassf-Hippo) domain and central portion being composed Ras associated (RA) domain. Here, we investigate effect Rassf effectors on Mst1 function by mapping interaction various domains kinase using surface plasmon resonance (SPR). The results revealed that apart from which interacts to form heterodimers Rassf1A/5, N-terminal plays...
Targeting CD8+ T cells to recurrent tumor-specific mutations can profoundly contribute cancer treatment. Some of these are potential tumor antigens although they be displayed by non-spliced epitopes only in a few patients, because the low affinity mutated peptides for predominant HLA class I alleles. Here, we describe pipeline that uses large sequence variety proteasome-generated spliced and identifies epitope candidates, which carry binds HLA-I alleles with high affinity. They could used...
Abstract Antibiotic production and resistance pathways in Streptomyces are dictated by the interplay of transcriptional regulatory proteins that trigger downstream responses via binding to small diffusible molecules. To decipher mode DNA associated allosteric mechanism sub-class transcription factors induced γ-butyrolactones, we present crystal structure CprB complex with consensus element a resolution 3.25 Å. Binding results restructuring dimeric interface CprB, inducing pendulum-like...
The interaction between the receptor 4-1BB and its ligand 4-1BBL provides co-stimulatory signals for T-cell activation proliferation. However, differences in mouse human molecules might result differential engagement of this pathway. Here, we report crystal structure 4-1BB/4-1BBL complex, which together provided insights into molecular mechanism by m4-1BBL cognate recognize each other. Unlike all or tumor necrosis factor ligands that form noncovalent mostly trimeric assemblies, formed a...
Guanine deaminases (GDs) are important enzymes involved in purine metabolism as well nucleotide anabolism pathways that exhibit a high degree of fidelity. Here, the structural basis substrate specificity GDs was investigated by determining series X-ray structures NE0047 (GD from Nitrosomonas europaea) with nucleobase analogues and nucleosides. The demonstrated interactions GD active site tailor-made to accommodate only guanine any substitutions ring or introduction pyrimidine results...
Invariant natural killer T-cells (iNKT) are a glycolipid-responsive subset of T-lymphocytes that fulfill pivotal role in the immune system. The archetypical synthetic glycolipid, α-galactosylceramide (α-GalCer), whose molecular framework is inspired by group amphiphilic products, remains most studied antigen for iNKT-cells. Nonetheless, potential α-GalCer as an immunostimulating agent compromised fact this glycolipid elicits simultaneous secretion Th1- and Th2-cytokines. This has incited...
Protein kinases are important drug targets due to their involvement in several pathological conditions. Here, we demonstrate a strategy develop small molecule inhibitors by using peptidomimetic compounds as target for serine/threonine kinases. In this regard, employ unique approach modify peptides copper catalyzed 1,3-dipolar cycloaddition reaction between various azides and containing an alkyne moiety create triazole based library. Interestingly, these have yielded competent kinase...
NE0047 from Nitrosomonas europaea has been annotated as a zinc-dependent deaminase; however, the substrate specificity is unknown because of low level structural similarity and sequence identity compared to other family members. In this study, function was established guanine deaminase (catalytic efficiency 1.2 × 10(5) M(-1) s(-1)), exhibiting secondary activity towards ammeline. The structure in presence analogue 8-azaguanine also determined resolution 1.9 Å. crystallized homodimer an...
4‐1BB (CD137) is an inducible tumor necrosis factor receptor superfamily (TNFRSF) member and currently being targeted in cancer immunotherapy autoimmunity. interacts with its cognate ligand 4‐1BBL to promote T lymphocyte activation proliferation, but the dissimilarities mouse human molecules might result differential engagement of this pathway. To elucidate molecular mechanism underlying distinct behavior, we have determined crystal structures 4‐1BB/4‐1BBL complexes. Collectively, these...
ABSTRACT The interaction between the 4-1BB and its ligand 4-1BBL provides co-stimulatory signals for T cell activation proliferation, but differences in mouse human molecules might result differential engagement of this pathway. Here, we report crystal structure 4-1BB/4-1BBL complex, together provide insights into molecular recognition cognate receptor by m4-1BBL. In contrast to all or TNF ligands that form non-covalent mostly trimeric assemblies, m4-1BBL formed a novel disulfide linked...
Guanine deaminases (GDs) are important enzymes involved in both purine metabolism and nucleotide anabolism pathways. Here we present the molecular catalytic mechanism of NE0047 use information obtained to engineer specific enzyme activities. from Nitrosomonas europaea was found be a high fidelity guanine deaminase (catalytic efficiency 1.2 × 105 M–1 s–1). However; it exhibited secondary activity towards structurally non-analogous triazine based compound ammeline. The X-ray structure presence...
4-1BB (CD137) is a tumor necrosis factor receptor super family (TNFRSF) member that undergoes trimerization upon interacting with its trimeric ligand (4-1BBL) and form 4-1BB/4-1BBL hetero -hexameric complexes to promote antiviral immunity.4-1BB signaling greatly reduced in Galectin-9 (Gal-9) deficient mice, suggesting pivotal role of Gal-9 activation.Gal-9 tandem-repeat carbohydrate-binding protein engages receptors either facilitate the clustering individual hetero-hexameric or induce...