A5060136887- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Bone and Dental Protein Studies
- Polyamine Metabolism and Applications
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cellular transport and secretion
- Signaling Pathways in Disease
- RNA modifications and cancer
- Cardiac Arrest and Resuscitation
- Neonatal and fetal brain pathology
- Bacterial Infections and Vaccines
- Galectins and Cancer Biology
- Tissue Engineering and Regenerative Medicine
- bioluminescence and chemiluminescence research
- Agricultural Systems and Practices
- Bacteriophages and microbial interactions
- Vaccine Coverage and Hesitancy
- Muscle Physiology and Disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Chemical Safety and Risk Management
- Pharmacy and Medical Practices
- Mast cells and histamine
Imperial College London
2024
Indian Institute of Technology Bombay
2021-2023
University of Miami
2013-2020
National Center for Disease Control
1995-2020
Activation of an osteogenic transcriptional program contributes to the initiation aortic calcification in atherosclerosis.The role microRNAs regulating is understudied.We tested hypothesis that miR-30e regulates bone marrow-derived mesenchymal stem cells (MSCs), smooth muscle (SMCs), and ApoE 2/2 mice. Methods resultsIn aortas wild-type mice, we found highly expressed medial SMCs.In old transcripts are down-regulated inverse relation markers Runx2, Opn, Igf2.In vitro, over-expression reduced...
Cardiac myocyte hypertrophy, the main compensatory response to chronic stress in heart often progresses a state of decompensation that can lead failure. Osteopontin (OPN) is an effector for extracellular signalling induces growth and fibrosis. Although increased OPN activity has been observed stressed myocytes fibroblasts, detailed long term effects blocking on remain poorly defined. Targeting cardiac protein by RNA aptamer may be beneficial tuning down pathologic signalling. We aimed...
Arginyltransferase 1 (Ate1) mediates protein arginylation, a poorly understood posttranslational modification (PTM) in eukaryotic cells. Previous evidence suggest potential involvement of arginylation stress response and this PTM was traditionally considered anti-apoptotic based on the studies individual substrates. However, here we found that promotes cell death and/or growth arrest, depending nature intensity stressing factor. Specifically, yeast, mouse human cells, deletion or...
Inner ear spiral ganglion neurons were cultured from day 4 postnatal mice and loaded with a fluorescent Ca2+ indicator (fluo-4, -5F, or -5N). Pulses of infrared radiation (IR; 1,863 nm, 200 µs, 200-250 Hz for 2-5 s, delivered via an optical fiber) produced rapid, transient temperature increase 6-12°C (above baseline 24-30°C). These IR pulse trains evoked increases in both nuclear cytosolic concentration ([Ca2+]) 0.20-1.4 µM, simultaneous reduction [Ca2+] regions containing endoplasmic...
BackgroundMass gathering (MG) events are associated with public health risks. During the period January 14 to March 4, 2019, Kumbh Mela in Prayagraj, India was attended by an estimated 120 million visitors. An onsite disease surveillance established identify and respond outbreaks.MethodsA coordination committee for planning. Disease prioritized risk assessment done diseases/conditions based on epidemic potential, severity of illness, reporting requirement under International Health...
The ESCRT-III-like protein Vipp1 couples filament polymerization with membrane remodeling. It assembles planar sheets as well 3D rings and helical polymers, all implicated in mitigating plastid-associated stress. architecture of polymers remains unknown, do the geometric changes required to transition between polymeric forms. Here we show how cyanobacterial into morphologically-related spirals on membranes vitro. converge form a central ring similar those described budding. Cryo-EM...
Summary ESCRT-III family proteins build dynamic filaments that remodel membrane. Although the transition of planar into 3D membrane budding structures is fundamental for their function, geometric changes in polymer architecture driving remain obscure. Here we show how bacterial Vipp1 polymerises sheets and spirals on The converge to form a central ring like those known bud To probe morphs between polymers, determine multiple helical filaments. As well as describing filament constriction...
The NtrC family of proteins senses external stimuli and accordingly stimulates stress virulence pathways via activation associated σ54-dependent RNA polymerases. However, the structural determinants that mediate this are not well understood. Here, we establish using computational, structural, biochemical, biophysical studies MopR, an protein, harbors a dynamic bidirectional electrostatic network connects phenol pocket to two distal regions, namely “G-hinge” “allosteric linker.” While G-hinge...
Mild therapeutic hypothermia is protective against several cellular stresses, but the mechanisms underlying this protection are not completely resolved. In present study, we used an in vitro model to investigate whether at 33°C applied following a peroxide-induced oxidative stress would protect PC12 cells. A 1-hour exposure tert-butyl peroxide increased cell death measured 24 hours later. This was dose-dependent range of 100-1000 μM with ∼50% observed from 500 exposure. Cell survival/death...
Reporter methods to quantitatively measure the efficiency and specificity of genome editing tools are important for development novel techniques successful applications available ones. However, existing have major limitations in sensitivity, accuracy, and/or readiness vivo applications. Here, we aim develop a straight-forward method by using nucleotide insertion/deletion resulted from editing. In this system, target sequence with frame-shifting length is inserted after start codon cerulean...
Specialized sensing mechanisms in bacteria enable the identification of cognate ligands with remarkable selectivity highly xenobiotic-polluted environments where these are utilized as energy sources. Here, via integrating all-atom computer simulation, biochemical assay, and isothermal titration calorimetry measurements, we determine molecular basis MopR, a phenol biosensor's complex selection process ligand entry. Our results reveal set strategically placed filters along entry pathway MopR....
The current EPI methodology for identifying immunization coverage is simple and easy to carry out under field conditions gives a good idea about coverage. However, it not useful local managers. It does identify small health units with poor performance. Information on performance at the level vital enhance overall Estimation of area basis can be made by Lot Quality Assurance Sampling (LQAS). LQAS was used in nine sub-centres district Saharanpur. found feasible identified seven Although may...
The NtrC family of AAA+ proteins are bacterial transcriptional regulators that control σ54-dependent RNA polymerase transcription under certain stressful conditions. MopR, which is a member this family, responsive to phenol and stimulates its degradation. Biochemical studies understand the role ATP in oligomerization allosteric regulation, described here, show MopR undergoes concentration-dependent dimers assemble into functional hexamers. occurs nucleation-dependent manner with tetrameric...
Abstract Specialized sensing mechanisms in bacteria enable the identification of cognate ligands with remarkable selectivity highly xenobiotic-polluted environments, where these are utilized as energy sources. Here, via an integrated all-atom computer simulation, biochemical assay and isothermal titration calorimetry approaches we determine molecular basis MopR, a phenol biosensor’s complex selection process ligand entry. Our results reveal set strategically placed filters along entry...
Objective: Osteopontin (OPN) expression increases in the heart during hypertrophy and failure aorta atherosclerosis. OPN KO mice were reported to have a reduced hypertrophic response after trans-aortic constriction (TAC), plaque burden on an APOE-/- background. We hypothesized that RNA aptamer targeting protein would effectively modulate responses TAC atherogenesis. Method Results: C57BL6 male subjected sham or surgeries. injected starting day1 with mutant aptamer. At 4wks, treatment...
ABSTRACT NtrC family of proteins sense external stimuli and accordingly stimulate stress virulence pathways via activation associated σ54-dependent RNA polymerases. Here, we establish that MopR, an protein, harbors a dynamic bi-directional electrostatic network connects the phenol pocket to two distal regions, namely “G-hinge” “allo-steric-linker”. While G-hinge influences entry phenol, allosteric-linker passes signal downstream ATPase domain. Phenol binding induces rewiring connections by...
The present study was undertaken to evaluate the CSF ADA activity in patients of tubercular meningitis and efficacy differentiating various types meningitis. A total 97 including 18 controls were enrolled study. Further, 79 divided into three groups depending upon clinical picture investigatory findings i.e. pyogenic (n=20), (n=35) viral (n=24). In this level determined tuberculous, admitted medical wards Subharti Hospital. This concluded that cerebrospinal fluid (CSF) can be reliably used...
Medical students confront with significant academic, psychological, & existential stressors throughout their professional training. Studies have shown that examination stress can a effect on blood cell parameters. The present study was designed to examine the of different type cells i.e RBC, platelets, haematocrit, TLC, eosinophil, basophil, lymphocyte, monocytes and leucocytes. changes occurs are increase in neutrophils whereas lymphocytes, eosinophils decrease number.
Heart failure with preserved ejection fraction (HFpEF) accounts for more than 50% of all HF cases. HFpEF patients manifest normal or mildly reduced left ventricle (LV) (LVEF), LV hypertrophy, diastolic dysfunction, myocardial stiffness, cardiac fibrosis, hypertension, nephropathy and sudden death. There is no appropriate animal model HFpEF. The Alport Col4a3 -/- mouse, a nephropathy, hypertension early death, develops cardiovascular pathology that yet to be well characterized. We elected...
Abstract Sirtuins (SIRTs) are NAD+ dependent deacetylases, which play an important role in cancer cell survival and resistance to chemotherapy. SIRT1, the most studied sirtuin, is overexpressed many drug resistant cancers including neuroblastoma. SIRTs mediate cellular processes by deacetylating histone non-histone proteins those involved oxidative stress response, DNA repair apoptosis. Here we define a novel for Sirts modulating unfolded protein response (UPR) following endoplasmic...
Arginylation is a protein posttranslational modification (PTM) which leads to addition of an extra arginine at the N‐terminus target proteins and peptides. This process evolutionarily conserved, mediated by arginyltransferase1 (Ate1). While earlier studies found that overall arginylation activity often increases during stressing conditions including heat shock, oxidative stress, injury, little known about role or Ate1 in stress response. Here we report promotes growth arrest cell death,...