A5011649274- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Genomics and Chromatin Dynamics
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Genetic Syndromes and Imprinting
- Liver Disease Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- Bryophyte Studies and Records
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Gut microbiota and health
- Gene expression and cancer classification
- Bacteriophages and microbial interactions
- Burkholderia infections and melioidosis
- Plant nutrient uptake and metabolism
- Vasculitis and related conditions
- Dietary Effects on Health
- Radiomics and Machine Learning in Medical Imaging
- Drug-Induced Adverse Reactions
- Prenatal Screening and Diagnostics
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Renal and related cancers
University of North Dakota
2021-2025
Gregor Mendel Institute of Molecular Plant Biology
2018-2024
Austrian Academy of Sciences
2018-2024
Sanford Health
2024
Vienna Biocenter
2018-2023
Medical University of Vienna
2022-2023
Centre for Genomic Regulation
2023
Veterans Health Administration
2021
University of British Columbia
2015-2016
Canada's Michael Smith Genome Sciences Centre
2006
Genome packaging by nucleosomes is a hallmark of eukaryotes. Histones and the pathways that deposit, remove, read histone modifications are deeply conserved. Yet, we lack information regarding chromatin landscapes in extant representatives ancestors main groups eukaryotes, our knowledge evolution chromatin-related processes limited. We used bryophyte Marchantia polymorpha, which diverged from vascular plants circa 400 mya, to obtain whole chromosome genome assembly explore landscape...
Chromatin regulation of eukaryotic genomes depends on the formation nucleosome complexes between histone proteins and DNA. Histone variants, which are diversified by sequence or expression pattern, can profoundly alter chromatin properties. While variants in H2A H3 families well characterized, extent diversification H2B is less understood. Here, we report a systematic analysis family plants, have undergone substantial divergence during evolution each major group plant kingdom. By...
The mobility of transposable elements (TEs) contributes to evolution genomes. Their uncontrolled activity causes genomic instability; therefore, expression TEs is silenced by host are marked with DNA and H3K9 methylation, which associated silencing in flowering plants, animals, fungi. However, distantly related groups eukaryotes, H3K27me3 deposited the Polycomb repressive complex 2 (PRC2), an epigenetic mark gene plants animals. direct PRC2 has so far only been shown one species ciliates. To...
Abstract Evolutionary mechanisms underlying innovation of cell types have remained largely unclear. In multicellular eukaryotes, the evolutionary molecular origin sperm differentiation is unknown in most lineages. Here, we report that algal ancestors land plants, changes DNA-binding domain ancestor MYB transcription factor DUO1 enabled recognition a new cis- regulatory element. This event led to motile sperm. After neo-functionalization, acquired lineage-specific expression common plants....
Sex determination is a central process for sexual reproduction and often regulated by sex determinant encoded on chromosome. Rules that govern the evolution of chromosomes via specialization degeneration following have been well studied in diploid organisms. However, distinct predictions apply to organisms where determined haploid phase life cycle: both chromosomes, female U male V, are expected maintain their gene functions, even though non-recombining. This contrast X-Y (or Z-W) asymmetry...
Abstract Goose can develop severe hepatic steatosis without overt injury, thus it may serve as a unique model for uncovering how steatosis-related injury is prevented. To identify the markedly prosteatotic and protective mechanisms, we performed an integrated analysis of liver transcriptomes gut microbial metagenomes using samples collected from overfed normally-fed geese at different time points. The results indicated that fatty transcriptome, initially featuring ‘metabolism’ pathway, was...
Complex mechanisms regulate gene dosage throughout eukaryotic life cycles. Mechanisms controlling have been extensively studied in animals, however it is unknown how generalizable these are to diverse eukaryotes. Here, we use the haploid plant
Histone post-translational modifications (hPTMs) are key regulators of chromatin states, influencing gene expression, epigenetic memory, and transposable element repression across eukaryotic genomes. While many hPTMs evolutionarily conserved, the extent to which states they define similarly preserved remains unclear. Here, we developed a combinatorial indexing ChIP-seq method simultaneously profile specific diverse lineages, including amoebozoans, rhizarians, discobans, cryptomonads. Our...
Diversification of histone variants is marked by the acquisition distinct motifs and functional properties through convergent evolution.1-4 H2A are distinguished specific C-terminal tend to be segregated within defined domains genome.5,6 Whether evolution these pre-dated segregation mechanisms or vice versa has remained unclear. A suitable model address this question variant H2A.W, which evolved in plants a KSPK motif7 tightly associated with heterochromatin.4 We used fission yeast, where...
Previous studies suggest that prolactin receptor (Prlr) is a potential causative gene for chicken early- (EF) and late-feathering (LF) phenotypes. In this study, we evaluated candidate genes trait determined the expression of 3 genes, including Prlr, sperm flagellar protein 2 (Spef2), their fusion gene, in skins one-day-old EF LF chicks using RTqPCR. Data indicated Prlr skin did not show significant difference between chicks, suggesting may be suitable gene. contrast, Spef2 displayed (P <...
Abstract Genomic imprinting results in the biased expression of alleles depending on if allele was inherited from mother or father. Despite prevalence sexual reproduction across eukaryotes, is only found placental mammals, flowering plants, and some insects, suggesting independent evolutionary origins. Numerous hypotheses have been proposed to explain selective pressures that favour innovation imprinted gene each differs their experimental support predictions. Due lack investigation land...
Summary Relatively little is known about species‐level genetic diversity in flowering plants outside the eudicots and monocots, it often unclear how to interpret patterns lineages with whole‐genome duplications. We addressed these issues a polyploid representative of Hydatellaceae, part water‐lily order Nymphaeales. examined transcriptome Trithuria submersa for evidence recent duplication, applied transcriptome‐derived microsatellite (expressed‐sequence tag simple‐sequence repeat ( EST ‐ SSR...
Summary The mobility of transposable elements (TEs) contributes to evolution genomes 1,2 . Meanwhile, their uncontrolled activity causes genomic instability and therefore expression TEs is silenced by host 3,4 are marked with DNA H3K9 methylation that associated silencing in flowering plants 5 , animals, fungi 6 Yet, distantly related eukaryotes instead H3K27me3 deposited the Polycomb Repressive Complex 2 (PRC2) 7–11 an epigenetic mark gene multicellular 12–15 It was proposed ancestral PRC2...
Abstract In mammals, genomic imprinting results from different sets of epigenetic marks that distinguish the parental origins loci in progeny. Epigenetic reprogramming is necessary to establish a totipotent cell state. The consecutive erasure and deposition new occurs alongside major life stage transitions including gametogenesis fertilization. However, despite occurring concomitantly with gametogenesis, role meiosis has received little attention. To address this question, we use model...
Around the time of fertilization and early embryogenesis, epigenetic marks parental genomes are reprogrammed to produce new chromatin landscape progeny. In most animals plants, embryogenesis tightly coupled meiosis. To address question whether reprogramming is driven by meiosis, here we studied liverwort Marchantia polymorpha in which meiosis entirely separate space from embryogenesis. Marchantia, show that reshuffled over course This followed establishment governs vegetative plant...
Summary Genome packaging by nucleosomes is a hallmark of eukaryotes. Histones and the pathways that deposit, remove, read histone modifications are deeply conserved. Yet, we lack information regarding chromatin landscapes in extant representatives ancestors main groups eukaryotes our knowledge evolution related processes limited. We used bryophyte Marchantia polymorpha , which diverged from vascular plants 400 Mya, to obtain whole chromosome genome assembly explore landscape...