MicroRNA-21 (miR-21) is recognized as an oncomir and shows up-regulation in many types of human malignancy. The aim this study was to investigate the association miR-21 expression associated with HPV infection normal abnormal cervical tissues. Cervical tissue samples different cytological or histopathological grades were investigated for by PCR programmed cell death, protein 4 (PDCD4) using quantitative real-time (qRT-PCR). Laser capture microdissection (LCM) stromal epithelial tissues situ...

Cervical cancer continues to pose a challenge the health of Thai women, as second most common after breast cancer. Since high-risk human papillomavirus (HPV) types are main cause for cervical cancer, screening and HPV vaccination necessary reduce incidence this disease. At present, World Health Organization hopes 4 or less cases per 100,000 women-years using 90%- 70%-90% intervention by 2030. The first involves vaccinating 90% women aged 15 years with vaccine. 70% between ages 35 45...

Cervical squamous cell carcinoma (CSCC) is a major cause of female mortality worldwide. This study has examined epidermal growth factor receptor (EGFR) pathway markers that represent actionable pharmacological targets. HPV16 positive CSCCs (n = 105 patients) from Madhya Pradesh, India were screened for KRAS and PIK3CA mutations by PNA-clamp real-time PCR. Immunohistochemistry (IHC) was performed EGFR, PIK3CA, PTEN, phospho-AKT, phospho-mTOR phospho-44/42 MAPK (ERK1/2). detected in 0/91 (0%)...

Cutibacterium acnes is one of the common multifactorial causes that play an important role in pathophysiology acne vulgaris. We aimed to develop novel antimicrobial peptides for reduction hypercolonization.Six cationic were derived by de novo designation. The and cytotoxic activities investigated. peptide conformation was determined circular dichroism spectrometry. effects evaluated using scanning electron microscopy (SEM), transmission (TEM) DNA-binding ability assay.Among designed...

The current practice of determining histologic grade with a single molecular biomarker can facilitate differential diagnosis but cannot predict the risk lesion progression. Cancer is caused by complex mechanisms, and no both make accurate diagnoses progression risk. Modelling using multiple biomarkers be used to derive scores for prediction. Mathematical models (MMs) may capable making predictions from data. Therefore, this study aimed develop MM–based predicting precancerous cervical...

Background: Variants of human papillomavirus (HPV) show more oncogenicity than do prototypes. The HPV16 Asian variant (HPV16As) plays a major role in cervical cancer populations. Some amino acid changes the E6 protein variants affect functions such as p53 interaction and host immune surveillance. This study aimed to investigate activities HPV16As on modulation expression miRNA-21 well interferon regulatory factors (IRFs) 1, 3, 7 c-fos. Materials Methods: Vectors expressing (E6D25E) or...

Major depressive disorder (MDD) is a silent global health problem that can lead to suicide. MDD development suggested result from numerous risk factors, including genetic factors. A precise tool for diagnosis currently not available. Recently, inflammatory processes have been identified as being strongly involved in and the reactivation of human herpesvirus type 6 (HHV-6), upregulating cytokines such TNF-α, which are associated with MDD. Therefore, this study aimed determine association...

Major depressive disorder (MDD) is a global health concern with complex etiology involving genetic, environmental and infectious factors. The exact cause of MDD remains unknown. present study explored the association between genetic factors, human herpesvirus 6 (HHV-6) MDD. analyzed single nucleotide polymorphisms (SNPs) HHV-6 viral load in oral buccal samples from patients (with without blood relatives MDD) healthy controls. used high-resolution melt analysis to examine rs40184 (C>T) solute...

Water can contain pathogenic viruses. Many studies on RNA virus sources have shown that water transmit them. However, there are few reports DNA transmission through water, such as adenovirus, which pose a widespread public health risk. Therefore, this study aimed to show waterborne viral by detecting viruses in pooled human whole blood samples, tap and natural from Mueang District, Phayao Province, Thailand, using metagenomic approach. Viral prevalence samples was measured polymerase chain...

Background The incidence of oral cancers associated with human papillomavirus (HPV) has been increasing in recent years. Therefore, it is necessary to elucidate HPV prevalence cells and exposure risk factors various age groups. Methods Oral rinse samples from healthy individuals northern Thailand were investigated for genotyped using the polymerase chain reaction (GP5+/6+ primers) DNA sequencing PCR products. Results Samples collected 594 participants between 4 60 years age. was detected...

Breast cancer is the most prevalent and also leading cause of death in women worldwide. A comprehensive understanding breast risk factors their incidences useful information for prevention control planning. The present study aimed to provide on single nucleotide polymorphisms (SNPs) copy number variations (CNVs) cancer, allele frequency two SNPs cancer-related genes BRCA1 DNA repair associated (

The 12% Allium cepa extract topical formulation, with proven clinical benefits, targets improved surgical scar appearance. This study aims to comprehensively assess the physicochemical characteristics and stability of a scar-reducing formulation (15% cepa-micellar extract, 0.01% quercetin). In vitro-release skin permeation studies were conducted, alongside FTIR DSC analyses elucidate mechanism. Quercetin remaining at 25 40 °C ranged from 99 100% (day 60) 101 104% 90), while antioxidant...

The objective of this study was to develop novel invaethosomes (I-ETS) and invaflexosomes (I-FXS) enhance the dermal delivery clotrimazole (CZ). Twenty model CZ-loaded I-ETS I-FXS formulations were created according a face-centered central composite experimental design. vesicle containing constant concentration 0.025% w/v CZ various amounts ethanol, d-limonene, polysorbate 20 as penetration enhancers prepared using thin film hydration method. physicochemical characteristics, skin...

Phusang warm pond or hot spring (Thailand) has a temperature of approximately 36–38 °C and may be suitable for bacterial growth. However, data on the microbiota effectiveness this water remain obscure. Therefore, study aimed to evaluate characteristics by clarifying microorganisms viral pathogens present. Cytotoxicity, irritation, liposome uptake, antimicrobial anti-inflammatory effects were evaluated. The levels trace elements such as sodium (Na), calcium (Ca), strontium (Sr) confirmed. was...

The Phusang waterfall is located in National Park Phayao province, Thailand. robustness of Phusang's warm regionally recognized as the only one with a temperature range 35-36 °C which makes it an outstanding place to visit Surprisingly, originates from pool (hot spring). However, data about bacterial community and characteristics this water are still obscure. Therefore, study investigated hot spring. Tests determine its physical such pH, color turbidity were performed. Trace elements sodium,...

Coronavirus disease (COVID-19) cases are continuing to rise around the world, with more than 607 million confirmed and 6.51 deaths as of September 2022. The World Health Organization (WHO) has emphasized importance hygiene in ongoing COVID-19 pandemic, especially elderly bedridden patients. use no-rinse shampoo represents a simple change hair-cleansing products. Daily hospital hair washing for elderly, bedridden, ICU patients would be readily adopted. objective this study was design optimize...

Abstract Background Invasive cervical carcinoma (ICC) remains a major cause of female mortality worldwide claiming the lives around 275,000 women each year. Human papillomavirus (HPV) infection is known ICC. Whilst advances have been made in prevention ICC by incorporating HPV testing into screening programs and development vaccines, relatively little progress has understanding cellular pathway abrogation significance for treatment. In this study, upstream elements PI3K RAS signaling...

Niosomes are more stable and economical than liposomes. In previous studies, conventional niosomes have shown advantages for transdermal delivery of bioactive compounds from rice bran. The objective this study was to compare nanovesicle formulations (conventional pH-sensitive niosomes) containing oryzanol (OZ) bran oil (RBO). formulation parameters were varied the types model drugs (OZ pure compound RBO), content RBO loading (low, medium, high), niosomes). physicochemical properties, e.g.,...