Zhendong Cai

ORCID: 0000-0003-1702-1185
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About
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Research Areas
  • Probiotics and Fermented Foods
  • Identification and Quantification in Food
  • Microbial Inactivation Methods
  • Biochemical Analysis and Sensing Techniques
  • Antifungal resistance and susceptibility
  • Protein Hydrolysis and Bioactive Peptides
  • Food Supply Chain Traceability
  • Microbial Metabolites in Food Biotechnology
  • Gut microbiota and health
  • Food Quality and Safety Studies
  • Mycotoxins in Agriculture and Food
  • Enzyme Production and Characterization
  • Trace Elements in Health
  • Analytical chemistry methods development
  • Cancer-related molecular mechanisms research
  • Advanced Chemical Sensor Technologies
  • Polysaccharides and Plant Cell Walls
  • Isotope Analysis in Ecology
  • Aluminum toxicity and tolerance in plants and animals
  • GABA and Rice Research
  • Environmental DNA in Biodiversity Studies
  • Microencapsulation and Drying Processes
  • Immune Response and Inflammation
  • RNA modifications and cancer
  • Nuts composition and effects

Ningbo University
2018-2025

Hainan University
2024

Beijing Technology and Business University
2024

University of Nottingham Ningbo China
2022

Shanghai First People's Hospital
2016-2021

Hubei University
2021

Beijing University of Agriculture
2019

Nanjing Normal University
2015-2019

Central Hospital of Putuo District
2019

Shanghai Jiao Tong University
2016-2017

Lactobacillus are normal inhabitants of the gastrointestinal tract and confer a variety health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in cell membrane, is key molecule probiotic-host crosstalk. Through characterization structural characteristics LTA molecules derived from plantarum A3, reuteri DMSZ 8533, acidophilus CICC 6074, there exists some heterogeneity molecules, which perhaps contributes to distinguishable adhesion properties strains based on fluorescence...

10.3390/foods11111610 article EN cc-by Foods 2022-05-30

In this study, fermentation with Lactobacillus plantarum and Saccharomyces cerevisiae was applied to improve the flavor of cured duck leg meat. Odor taste evaluations, lipid oxidation, volatile substances, protein degradation were determined investigate effects microbial on improvement. The results showed that utilization L. represented most significant effect peroxidation inhibition (the lowest value thiobarbituric acid reactive substances highest content polyunsaturated fatty acids) also...

10.1016/j.psj.2020.06.019 article EN cc-by-nc-nd Poultry Science 2020-07-02

Abstract BACKGROUND The traditional screening method for umami peptide, extracted from porcine bone, was labor‐intensive and time‐consuming. In this study, the rapid molecular mechanism of peptide investigated. RESULTS This article showed that a more precisely with composite machine learning docking used to screen potential bone. As reference, 24 reported peptides were predicated by learning, accuracy 86.7%. sequences bone screened UMPred‐FRL, Umami‐MRNN Demo, provide further screening....

10.1002/jsfa.12319 article EN Journal of the Science of Food and Agriculture 2022-11-06

Lactobacillus strains fermentation of broccoli as a good source bioactive peptides has not been fully elucidated. In this work, the peptide composition fermented by L. plantarum A3 and rhamnosus ATCC7469 was analyzed peptidomics to study protein digestion patterns after different strains. Results showed that water-soluble proteins such rubisco were abundant sources peptides, which triggered sustained release main target hydrolysis. addition, 17 novel anti-inflammatory identified virtual...

10.3389/fnut.2022.1118900 article EN cc-by Frontiers in Nutrition 2023-01-11

Copper (Cu) and zinc (Zn) are essential nutrients for both pathogens hosts; however, their excess accumulation is toxic all cells. The Aspergillus transcription factor AceA has been identified having function Cu detoxification through up-regulation of the expression P-type ATPase, CrpA. Here, we demonstrate that fumigatus CrpA involved in Zn a putative metallothionein AfCrdA plays major detoxification, transporter AfZrcA dominant role but three members transcriptionally dependent on AfAceA....

10.1111/cmi.12864 article EN Cellular Microbiology 2018-05-30

Osteosarcoma is the most common malignancy of bone. Intratumoral hypoxia occurs in many solid tumors, where it associated with development aggressive phenotype. ANRIL has been shown to be a long noncoding RNA that facilitates progression number malignancies. Yet, few studies have explored expression pattern osteosarcoma and effect on ANRIL. We evaluated levels tissues, adjacent normal tissues cells quantitative real-time polymerase chain reaction. Multiple approaches including luciferase...

10.1186/s12935-016-0349-7 article EN cc-by Cancer Cell International 2016-09-23

This study aims to identify lactic acid bacteria (LAB) isolates possessing physiological characteristics suitable for use as probiotics in yogurt fermentation. Following and bile salt tolerance tests, Lactiplantibacillus plantarum (NUC08 NUC101), Lacticaseibacillus rhamnosus (NUC55 NUC201), paracasei (NUC159, NUC216, NUC351) were shortlisted based on intraspecies distribution further evaluation. Their probiotic properties, including transit tolerance, adhesion, autoaggregation, surface...

10.3168/jds.2023-24113 article EN cc-by Journal of Dairy Science 2023-12-21

This work investigated the effects of combined use thermosonication-preconditioned lactic acid bacteria (LAB) with addition ultrasound-assisted pineapple peel extracts (UU group) on post-acidification potential, physicochemical and functional qualities yogurt products, aimed at achieving prolonged preservation enhancing attributes. Accordingly, physical–chemical features, probiotic evaluation as revealed by adhesion properties, sensory assessment, acidification curves, contents major organic...

10.1016/j.ultsonch.2024.106857 article EN cc-by-nc Ultrasonics Sonochemistry 2024-03-25

Lipoteichoic acid synthase (LtaS) is crucial for the biosynthesis of lipoteichoic (LTA) in lactic bacteria (LAB), where LTA plays a key role bacterial adhesion, immune modulation, and maintaining cell integrity. This study explores regulation LtaS activity Lactiplantibacillus plantarum, examining effects factors such as temperature, pH, metal ions on enzyme activity. Molecular docking was used to identify critical amino acids at enzyme's active site, site-directed mutagenesis confirmed five...

10.1021/acs.jafc.4c08913 article EN Journal of Agricultural and Food Chemistry 2025-01-20

Identification of meat authenticity is a matter increasing concerns due to religious, economical, legal, and public health reasons. However, little known about the inspection eight species in one tube reaction technological challenge multiplex polymerase chain (PCR) techniques. Here, developed PCR method can simultaneously authenticate including ostrich (753 bp), cat (564 goose (391 duck (347 chicken (268 horse (227 dog (190 sheep (131 bp). The detectable deoxyribonucleic acid (DNA) contents...

10.3389/fnut.2022.979977 article EN cc-by Frontiers in Nutrition 2022-09-16

Hyperuricemia is a metabolic disorder caused by abnormal purine metabolism, resulting in abnormally high serum uric acid. In this study, novel <i>Levilactobacillus brevis</i> PDD-5 isolated from salty vegetables was verified with the function of alleviating hyperuricemia. The relevant effects <i>L. lowering acid were analyzed <i>in vitro</i> and vivo</i> experiments. results showed that has (68.86 ± 15.46)% inosine uptake capacity (95.75 3.30)% guanosine vitro</i>. Oral administration to...

10.26599/fshw.2022.9250077 article EN cc-by-nc-nd Deleted Journal 2023-04-14

The ability to survive in the harsh gastrointestinal tract (GIT) environment is essential for Lactobacillus reuteri (L. reuteri) exhibiting beneficial effects. In this study, we found that hydrophobicity and auto-aggregation of L. SH23 were significantly decreased biofilm production was also when passes through simulated GIT. Furthermore, according comparative transcriptome analysis, gene expression involved cell envelope, metabolic processes, common stress response, regulatory systems,...

10.1021/acs.jproteome.0c00933 article EN Journal of Proteome Research 2021-03-11

Multiplex PCR methods have been frequently used for authentication of meat product adulteration. Through screening new species-specific primers designed based on the mitochondrial DNA sequences, a septuple method is ultimately developed and optimized to simultaneously detect seven species including turkey (110 bp), goose (194 pig (254 sheep (329 beef (473 chicken (612 bp) duck (718 in one reaction. The proposed has validated be specific, sensitive, robust inexpensive. Taken together, assay...

10.3390/foods10051083 article EN cc-by Foods 2021-05-13
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