Glycolipids were extracted from the red alga Osmundaria obtusiloba Southeastern Brazilian coast. The acetone insoluble material was with chloroform/methanol and lipids, enriched in glycolipids, fractionated on a silica gel column eluted chloroform, then methanol. Three major orcinol-positive bands found methanol fractions, being detected by thin layer chromatography. structures of corresponding glycolipids elucidated ESI-MS 1H/13C NMR analysis, basis their tandem-MS behavior HSQC, TOCSY...

Organic extracts of 36 species marine algae (sixteen Rhodophyta, eight Ochrophyta and twelve Chlorophyta) from seven locations on the Brazilian coast were evaluated for their anti-HSV-1 anti-HSV-2 activity resistant to Acyclovir (ACV). Activity tests in crude extracts, followed by identification major compounds present, performed all species. The chemical profiles obtained ¹H-NMR 13C-NMR spectroscopy. percentage with antiviral was higher HSV-1 (86.1%) than HSV-2 (55.5%). green Ulva fasciata...

Kalanchoe daigremontiana (Crassulaceae) is a medicinal plant native to Madagascar. The aim of this study was investigate the flavonoid content an aqueous leaf extract from K. (Kd), and assess its antiherpetic potential. major flavonoid, kaempferol 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside (1), isolated AcOEt fraction (Kd-AC). BuOH-soluble afforded quercetin (2) new 2)-α-l-rhamnopyranoside-7-O-β-d-glucopyranoside (3), named daigremontrioside. crude extract, Kd-AC fraction, flavonoids...

To evaluatein vitro the antibacterial activity, antibiofilm effect and cytotoxic potential of mouthwashes containing Brazilian red propolis with or without fluoride.The minimum inhibitory bactericidal concentrations (MIC MBC) against S. mutans, sanguinis, salivarius L. casei were determined for RPE mouthwashes. A cariogenic biofilm aforementioned bacteria was formed over cellulose membrane disks (N = 30, 13 mm), which submitted 1 min to following mouthwashes: plain mouthwash base; 0.05% NaF;...

Bacterial infections associated with healthcare are a challenge on global scale due to the high morbidity and mortality rates, especially those caused by multidrug-resistant isolates. Hospital textiles abiotic surfaces that may serve as means of disseminating persisting microorganisms in hospitals, can remain viable these for up months. In this study, we employed green synthesis approach utilizing guava leaf extract (Psidium guajava) produce silver nanoparticles, which were then incorporated...

Leishmaniasis is one of the most neglected tropical diseases, representing a group parasitic diseases worldwide spread, occurring in 88 and subtropical countries. Approximately 350 million people live areas active transmission leishmaniasis, with about 1-2 estimated new cases every year. More than 90% cutaneous appear developing Efforts to improve therapeutic arsenal against leishmaniasis have led search for cheap range drugs. In this study, nerolidol-rich essential oil from Piper...

We evaluated the antiviral activity of marine alga, Ulva fasciata, collected from Rasa beach and Forno beach, Búzios, Rio de Janeiro, Brazil on replication human metapneumovirus (HMPV). The algae extracts were prepared using three different methodologies to compare groups chemical composites obtained through these methodologies. Four out six inhibited nearly 100% viral replication. results demonstrated that majority (five six) possess virucidal therefore have ability interact with...

To evaluate the influence of pH levels on interarch elastics with regard to force decay and cytotoxicity.One nonlatex (NLAO) group one latex (LAO) were tested (n = 10). Elastics stretched 25 mm held for 1, 6, 12, 24 hours in artificial saliva solutions 5.0, 6.0, 7.5. Force magnitudes measured at activation. The cytotoxicity assay was performed using cell cultures (L929 mouse fibroblast line), which subjected viability test neutral red ("dye-uptake"). assessed analysis variance, Sidak method,...

In this paper, we evaluated the antiviral activity against HMPV replication of crude extract marine algae Stypopodium zonale and two meroditerpenoids obtained from it, atomaric acid epitaondiol, a methyl ester derivative acid. Their selectivity indexes were 20.78, >56.81, 49.26 12.82, respectively. Compared to ribavirin, substances showed relatively low cytotoxicity on LLC-MK2 cells, with significant activity, inhibiting at least 90% viral in vitro, which demonstrates potential these natural...

OBJECTIVES: Natural latex does not fall into the category of materials known to be entirely inoffensive. The purpose this in vitro study was test hypothesis that there is no difference cytotoxicity between elastics different colors and those from manufacturers. MATERIAL AND METHODS: Different intraoral (5/16 = 7.9 mm, mean load) were compared. sample divided 7 groups 24 each: Group T (TP Orthodontics, natural elastics, control); Groups U1, U2, U3, U4, U5 U6 (Uniden, colored namely, green,...

Couroupita guianensis is known in Brazil as 'Abricó-de-Macaco' and it has some attributes such as: antihypertensive, analgesic anti-inflammatory activities. This study evaluated the antimicrobial activity of ethanolic extract fractions C. flowers isolation bioactive component. These extracts were subjected to agar diffusion, MIC, TLC bioautography bacteria, filamentous fungi yeasts. Among EtOH extract, DCM fraction was most active, particularly against Methicillin-resistant Staphylococcus...

Verbascoside has been isolated form L. speciosa after several different chromatographic methods. After its purification, the structure unequivocally established using modern spectroscopic techniques. As for antiviral activity, maximum non toxic concentration and this used in anti herpes assay, vitro. Mechanism of action molecule regarding anti-herpes activity studied encompassing following assays: virucidal cellular receptor penetration assay intracellular order to understand natural product.

This study investigated the cytotoxicity exists between latex and non-latex Orthodontic elastomeric ligatures. Six ligatures (1 latex, 2 latex-free 3 polyurethane) from different manufacturers were divided into 6 groups of 15 elastics each: A (Latex-free, American Orthodontics), M (Polyurethane, Morelli), G (Polyurethane,GAC International), Te Tecnident), TP (Natural latex,TP Orthodontics) U (Latex-free,3M Unitek). The assay was performed using cell cultures (L929 mouse fibroblast line),...

Pseudallescheria boydii is a filamentous fungus that causes wide array of infections can affect practically all the organs human body. The treatment pseudallescheriosis difficult since P. exhibits intrinsic resistance to majority antifungal drugs used in clinic and virulence attributes expressed by this are unknown. study secretion molecules an important approach for understanding pathogenicity fungi. With task mind, we have shown mycelial cells were able actively secrete proteins into...

Four extracts from the seaweed Hypnea musciformis (Wulfen in Jacq.) J.V. Lamour. (Rhodophyta), collected directly its natural habitat or cultivated presence of phytohormones, were evaluated for their ability to inhibit replication acyclovir-resistant herpes simplex viruses types 1 (HSV-1) and 2 (HSV-2) strains. The main purpose was determinate whether these growth conditions would affect antiviral activity. Our results showed possibility improving anti-HSV activity by using algae cultured...

An aqueous extract and fraction from the marine sponge Petromica citrina have antibacterial activity. We performed a chemical biological characterization of antibiotic substance P. investigated its mode action on Staphylococcus aureus cells.The inhibitory activity was determined against 14 bacteria belonging to type strains clinical antibiotic-resistant strains. The fractionated under bioassay guidance bioactive identified by (1)H-NMR, (13)C-NMR mass spectra. MIC MBC this were determined....

OBJECTIVE: The aim of this study was to assess the in vitro cytotoxicity acrylic resins different colors over time. METHODS: Specimens were divided into 4 groups (n = 6) according color resin (Orto Class, Clássico, Campinas, São Paulo, Brazil): Group 1: clear resin; group 2: pink 3: blue and 4: green resin. All specimens fabricated mass manipulation technique submitted mechanical polishing protocol. control performed with an amalgam specimen (C+), a glass (C-) cell (CC). immersed Minimum...

Aim: The present study compared in vitro four types of latex intraoral elastics (5/16 = 7.9 mm, mean load) regarding their possible cytotoxic effects. Material and Methods: sample was allocated to groups 24 elastics: Group A (American Orthodontics), M (Morelli), T (TP U (Uniden). Cytotoxicity assays were performed using cell culture medium containing epithelioid-type cells (Hep-2 line) derived from human laryngeal carcinoma. Two methods for evaluating the cytotoxicity, agar overlay test...

Extracts from four species of Brazilian marine algae collected the Rio de Janeiro State coast were screened to determine inhibitory effect on HTLV-1-induced syncytium formation. Before performing inhibition assay 50% cytotoxic dose (CyD50) algal extracts was evaluated. The antiviral test carried out in HeLa cells co-cultured with HTLV-I infected T-cell line (C91/PL cells) presence concentration inferior that corresponding CyD50. It observed exposed Ulva fasciata extract showed 60.2% at a...