A5012817631- Chemotherapy-induced cardiotoxicity and mitigation
- HER2/EGFR in Cancer Research
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Cancer Treatment and Pharmacology
- Pluripotent Stem Cells Research
- Cardiomyopathy and Myosin Studies
- Ubiquitin and proteasome pathways
- Metabolism, Diabetes, and Cancer
- Cardiac Fibrosis and Remodeling
- Cellular Mechanics and Interactions
- Multiple Myeloma Research and Treatments
- Peptidase Inhibition and Analysis
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Viral Infections and Immunology Research
- Protein Degradation and Inhibitors
- Animal testing and alternatives
- Connexins and lens biology
- Adipose Tissue and Metabolism
- Cardiac Ischemia and Reperfusion
- Signaling Pathways in Disease
- Electron Spin Resonance Studies
- Pancreatic function and diabetes
- Cardiac Imaging and Diagnostics
University Hospital of Bern
2013-2023
Berner Augenklinik am Lindenhofspital
2022
University of Bern
2003-2020
École Polytechnique Fédérale de Lausanne
1999-2003
University of Lausanne
2003
Neurosciences Institute
2003
ETH Zurich
1997-2000
Institute of Cell Biology
1997-2000
Goethe University Frankfurt
2000
University of Zurich
1997
Purpose: Both cardiomyocytes and cardiac fibroblasts (CF) play essential roles in development, function, remodeling. Properties of 3D co-cultures are incompletely understood. Hence, co-culture CF was characterized, selected features compared with single-type 2D culture conditions. Methods: Human derived from induced-pluripotent stem cells (hiPSC-CMs) were obtained Cellular Dynamics or Ncardia, primary human ScienCell. Cardiac spheroids investigated using cryosections whole-mount confocal...
Titin, the largest myofilament protein, serves as a template for sarcomere assembly and acts molecular spring to contribute diastolic function. Titin is known be extremely susceptible calcium-dependent protease degradation in vitro. We hypothesized that titin an early event doxorubicin-induced cardiac injury occurs by activation of proteases, calpains. Treatment cultured adult rat cardiomyocytes with 1 or 3 μmol/liter doxorubicin 24 h resulted myocyte lysates, which was confirmed reduction...
Background — There is an increased incidence of heart failure in patients treated concurrently with anthracyclines and the chemotherapeutic anti-erbB2 agent trastuzumab (Herceptin). On basis our previous studies recombinant neuregulin-1β (NRG-1β), a ligand for erbB2 receptor tyrosine kinase, we hypothesized that activation by versus NRG-1 would cause differential effects on myocyte intracellular signaling as well anthracycline-induced myofibrillar injury might potentially account clinical...
Cardiomyocytes (CMs) are terminally differentiated cells in the adult heart, and ischemia cardiotoxic compounds can lead to cell death irreversible decline of cardiac function. As testing platforms, isolated organs primary from rodents have been standard research toxicology, but there is a need for better models that more faithfully recapitulate native human biology. Hence, new vitro model comprising advantages 3D culture availability induced pluripotent stem (iPSCs) origin was developed...
In this study, we investigated cardiomyocyte cytoarchitecture in a mouse model for dilated cardiomyopathy (DCM), the muscle LIM protein (MLP) knockout and substantiated several observations second DCM model, tropomodulin-overexpressing transgenic (TOT) mouse. Freshly isolated cardiomyocytes from both strains are characterized by more irregular shape compared with wild-type cells. Alterations observed at intercalated disks, specialized areas of mechanical coupling between cardiomyocytes,...
Among retroviral vectors, lentiviral vectors are unique in that they transduce genes into both dividing and nondividing cells. However, their ability to provide sustained myocardial transgene expression has not been evaluated.Multiply attenuated, self-inactivating lentivectors based on human immunodeficiency virus-1 contained the enhanced green fluorescent protein (EGFP) gene under transcriptional control of either cytomegalovirus (CMV) immediate-early enhancer/promoter, elongation...
Recent innovations in stem cell technologies and the availability of human induced pluripotent cell-derived cardiomyocytes (hiPSC-CMs) have opened new possibilities for studies drug testing on vitro. Still, there are concerns about precise nature such 'reprogrammed' cells. We performed an investigation using immunocytochemistry confocal microscopy several cellular features commercially available hiPSC-CMs. For some selected developmentally regulated or cardiac chamber-specific proteins, we...
The cytoplasmic domain of the transmembrane protein M-cadherin is involved in anchoring cytoskeletal elements to plasma membrane at cell-cell contact sites. Several members armadillo repeat family mediate this linkage. We show here that ARVCF, a member p120 (ctn) subfamily, ligand for M-cadherin, and characterize regions interaction detail. Complex formation an vivo environment was demonstrated (1) yeast two-hybrid screens, using cDNA library from differentiating skeletal muscle part tail as...
Neuregulin/ErbB2-induced kinase signaling provides essential survival and protection clues for functional integrity of the adult heart skeletal muscle. To define regulatory pathways involved in neuregulin-dependent muscle cell survival, we set out to map largely unknown transcript targets this growth/differentiation factor cardiocytes. Freshly isolated primary rat cardiocytes were treated 24 h with recombinant human neuregulin-1beta (NRG-1beta, 30 ng/ml). Transcript level alterations...
Sodium nitroprusside (SNP) is used clinically as a rapid-acting vasodilator and in experimental models donor of nitric oxide (NO). High concentrations NO have been reported to induce cardiotoxic effects including apoptosis by the formation reactive oxygen species. We therefore investigated SNP on myofibrillar cytoskeleton, contractility cell death long-term cultured adult rat cardiomyocytes at different time points after treatment. Our results show, that treatment first gradual increase...
Primary adult rat cardiomyocytes (ARC) in culture are shown to be a model system for cardiac cell hypertrophy vitro. ARC undergo process of morphological transformation and grow only by increase size, however, without loss the phenotype. The isolated cells spread establish new cell-cell contacts, eventually forming two-dimensional heart tissue-like synchronously beating sheet. reformation specific contacts (intercalated disks) is also between ventricular atrial using antibodies against gap...