A5083381526- Endometriosis Research and Treatment
- Wnt/β-catenin signaling in development and cancer
- Reproductive System and Pregnancy
- RNA Research and Splicing
- Uterine Myomas and Treatments
- Estrogen and related hormone effects
- Muscle Physiology and Disorders
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer-related gene regulation
- T-cell and B-cell Immunology
- Endometrial and Cervical Cancer Treatments
- Immunotherapy and Immune Responses
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Inflammatory mediators and NSAID effects
- Cellular transport and secretion
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Herpesvirus Infections and Treatments
- Genetic factors in colorectal cancer
- Signaling Pathways in Disease
- Molecular Biology Techniques and Applications
Goethe University Frankfurt
2012-2023
Heinrich Heine University Düsseldorf
2009
National Center for Tumor Diseases
2009
German Cancer Research Center
2009
Heidelberg University
2009
Scott & White Memorial Hospital
2009
Texas A&M University
2007-2009
Ono Pharmaceutical (United States)
2008
In-Q-Tel
1999
GTx (United States)
1999
Murine T lymphocytes sensitized in vitro against either allogeneic or syngeneic hapten-conjugated do differentiate into highly effective cytotoxic (CTL) (1-3). In vivo immunization of to the same antigens, however, results generation only marginal activity (1,4,5). Recently we found that weakness generated cytotoxicity is not due a failure antigen-induced T-cell sensitization but rather suppression differentiation CTL precursors CTL(6). keeping with this finding it was postulated suppressor...
Following muscle damage in adult vertebrates, myofibers can be regenerated from precursor cells (satellite cells). During this process, prenatal myogenesis is recapitulated to a large extent, both morphologically and molecularly. A putative morphoregulatory molecule involved M-cadherin (Mcad), calcium-dependent cell adhesion protein. The expression of Mcad was studied by immunofluorescence regenerating, denervated, normal mouse muscles. Our results demonstrate that present satellite muscle....
Cadherins, a multigene family of transmembrane glycoproteins, mediate Ca(2+)-dependent intercellular adhesion. They are thought to be essential for the control morphogenetic processes, including myogenesis. Here we report identification and characterization cDNA another member cadherin family, M-cadherin (M muscle), from differentiating muscle cells. The longest open reading frame cDNAs isolated contains almost entire coding region mature as determined by sequence homology known cadherins....
Glioblastoma spheroid cultures are enriched in tumor stem-like cells and therefore may be more representative of the respective primary tumors than conventional monolayer cultures. We exploited glioma culture model to find novel tumor-relevant genes.We carried out array-based comparative genomic hybridization derived from 20 glioblastomas. Microarray-based gene expression analysis was applied determine genes with differential compared normal brain tissue nonneoplastic spheroids The protein...
Endometriosis is a debilitating disease characterized by the presence of functional endometrial glandular epithelium and stroma outside uterine cavity that affects up to 20% women child-bearing age. Cyclooxygenase-2 (COX-2), rate-limiting enzyme in biosynthesis prostaglandin E2 (PGE2), highly expressed endometriotic tissues results increased concentrations peritoneal PGE2 women. In this study, we determined expression COX-2 protein ectopic eutopic endometria humans role cell survival,...
ABSTRACT Cadherins are a gene family encoding calcium-dependent cell adhesion proteins which thought to act in the establishment and maintenance of tissue organization. M-cadherin, one member family, has been found myogenic cells somitic origin during embryogenesis adult. These findings have suggested that M-cadherin is involved regulation morphogenesis skeletal muscle cells. Therefore, we investigated function fusion myoblasts into myotubes (terminal differentiation) culture. Furthermore,...
Antigen Leu-19 (Leu19-Ag), a 200- to 220-kDa surface glycoprotein, was originally identified on subset of human peripheral lymphocytes exhibiting non-major histocompatibility complex-restricted cytotoxicity. Here we report that monoclonal antibody (mAb-Leu19) labels structures in skeletal muscle: (i) satellite cells, which form the stem cell pool muscle fiber regeneration, both normal and diseased muscle; (ii) myotubes myotube projections regions repair; (iii) periodically organized...
FHL2 is a LIM-domain protein expressed in myoblasts but down-regulated malignant rhabdomyosarcoma cells, suggesting an important role of muscle development. To investigate the importance during myoblast differentiation, we performed yeast two-hybrid screen using cDNA library derived from induced for differentiation. We identified β-catenin as novel interaction partner and confirmed specificity association by direct vitro binding tests coimmunoprecipitation assays cell lysates. Deletion...
Tumor necrosis factor (TNF)-alpha is central to the endometriotic disease process. TNF-alpha receptor signaling regulates epithelial cell secretion of inflammation and invasion mediators. Because cells are a disease-inducing component lesion, we explored response 12Z immortalized human TNF-alpha. This report reveals impact disruption established TNF-alpha-induced cascades on expression biomarkers epithelial-mesenchymal transition (EMT) from cells. Note that show molecular potential soluble...
Endometriosis is a benign chronic gynecological disease of reproductive-age women characterized by the presence functional endometrial tissues outside uterine cavity. It an estrogen-dependent disease. Current treatment modalities to inhibit biosynthesis and actions estrogen compromise menstruation, pregnancy, reproductive health fail prevent reoccurrence There critical need identify new specific signaling modules for non-estrogen-targeted therapies endometriosis. In our previous study, we...
Significance Endometriosis is an inflammatory gynecological disease of reproductive age women associated with chronic pelvic pain and infertility. remains as the single major cause for hysterectomy in United States. The pathogenesis endometriosis enigma medicine. Current hormonal therapies undesirable side effects, health concerns, are unable to prevent recurrence disease. Results present study indicate that selective inhibition prostaglandin E2 (PGE 2 ) receptors EP2 EP4 suppresses growth...
Exported proteases of Helicobacter pylori (H. pylori) are potentially involved in pathogen-associated disorders leading to gastric inflammation and neoplasia. By comprehensive sequence screening the H. proteome for predicted secreted proteases, we retrieved several candidate genes. We detected caseinolytic activities such which released independently from type IV secretion system encoded by cag pathogenicity island (cagPAI). Among these, found serine protease HtrA (Hp1019), was previously...
Abstract Mice infected with herpes simplex virus develop little or no cytotoxic T lymphocyte (CTL) response. However, in lymph nodes (LN's) draining a local site HSV, antigen-specific CTL precursors are sensitized, which upon transfer to vitro culture conditions within 72 hr into effective CTL. The vivo blockade of differentiation can be overcome by cyclophosphamide, suggesting that cyclophosphamide-sensitive mechanism blocks the generation HSV-immune cytolytic activity is H-2 restricted and...
Molecules regulating morphogenesis by cell-cell interactions are the cadherins, a class of calcium-dependent adhesion molecules. One its members, M-cadherin, has been isolated from myoblast cell line (Donalies et al. [1991] Proc. Natl. Acad. Sci. U.S.A. 88:8024-8028). In mouse development, expression M-cadherin mRNA first appears at day 8.5 gestation (E8.5) in somites and postulated to be down-regulated developing muscle masses (Moore Walsh [1993] Development 117:1409-1420)....